LOCUS       sequence-178344-       12917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for HIV-1 env- integration into human AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12917)
  AUTHORS   Zotova A, Lopatukhina E, Filatov A, Khaitov M, Mazurov D
  TITLE     Gene Editing in Human Lymphoid Cells: Role for Donor DNA, Type of 
            Genomic Nuclease and Cell Selection Method.
  JOURNAL   Viruses. 2017 Nov 2;9(11). pii: v9110325. doi: 10.3390/v9110325.
  PUBMED    29099045
REFERENCE   2  (bases 1 to 12917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Viruses."; 
            date: "2017-11-2"; volume: "9(11). pii: v9110325. doi"; pages: " 
            10.3390/v9110325"
COMMENT     Sequence Label: pAAVS1-delta8.2R
FEATURES             Location/Qualifiers
     source          1..12917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..43)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(23..41)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(62..78)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(62..78)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(75..97)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    86..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(110..116,117..134,135..140))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    155..176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(293..310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(464..1052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(544..563)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1223..2014,2015..2083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1846..1865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2084..2188)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2214..2669)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2351..2372)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2563..2582
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2796..2818
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2810..2827
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2811..2827
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2837..2856
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2837..2855
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3060..3561
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     enhancer        3703..4082
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4083..4286
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4237..4257
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4283..4307
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4557..6059
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             5852..8863
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    8548..8665
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    10234..10467
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10652..10696
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             10990..11016
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     polyA_signal    11907..12041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11944..11963)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11998..12017
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    12049..12060
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     misc_feature    join(12085..12917,1..4)
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 ctctggtacc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
       61 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      121 ccgggagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      181 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      241 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
      301 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      361 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      421 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      481 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      541 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      601 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      661 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      721 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      781 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      841 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      901 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      961 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1021 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1081 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1141 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1201 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     1261 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     1321 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     1381 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     1441 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     1501 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     1561 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     1621 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     1681 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     1741 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     1801 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     1861 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     1921 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     1981 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2041 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     2101 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     2161 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     2221 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     2281 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     2341 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     2401 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     2461 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     2521 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     2581 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     2641 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     2701 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     2761 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     2821 ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggagctcc accgcggtgc
     2881 tttctctgac cagcattctc tcccctgggc ctgtgccgct ttctgtctgc agcttgtggc
     2941 ctgggtcacc tctacggctg gcccagatcc ttccctgccg cctccttcag gttccgtctt
     3001 cctccactcc ctcttcccct tgctctctgc tgtgttgctg cccaaggatg ctctttccgg
     3061 agcacttcct tctcggcgct gcaccacgtg atgtcctctg agcggatcct ccccgtgtct
     3121 gggtcctctc cgggcatctc tcctccctca cccaacccca tgccgtcttc actcgctggg
     3181 ttcccttttc cttctccttc tggggcctgt gccatctctc gtttcttagg atggccttct
     3241 ccgacggatg tctcccttgc gtcccgcctc cccttcttgt aggcctgcat catcaccgtt
     3301 tttctggaca accccaaagt accccgtctc cctggcttta gccacctctc catcctcttg
     3361 ctttctttgc ctggacaccc cgttctcctg tggattcggg tcacctctca ctcctttcat
     3421 ttgggcagct cccctacccc ccttacctct ctagtctgtg ctagctcttc cagccccctg
     3481 tcatggcatc ttccaggggt ccgagagctc agctagtctt cttcctccaa cccgggcccc
     3541 tatgtccact tcaggacagc atgtttgctg cctccaggga tcctgtgtcc ccgagctggg
     3601 accaccttat attcccaggg ccggttaatg tggctctggt tctgggtact tttatctgtc
     3661 ccctccaccc cacagtgggg acgcgtgaat tcgagctcgc ccgacattga ttattgacta
     3721 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     3781 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     3841 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     3901 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     3961 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     4021 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     4081 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     4141 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     4201 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     4261 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     4321 atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg
     4381 aacggtgcat tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagag
     4441 tctataggcc cacccccttg gcttcttatg cgacggatcg atcccgtaat aagcttcgag
     4501 gtccgcggcc gcgttgacgc gcacggcaag aggcgagggg cggcgactgg tgagagatgg
     4561 gtgcgagagc gtcagtatta agcgggggag aattagatcg atgggaaaaa attcggttaa
     4621 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     4681 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     4741 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     4801 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     4861 acaagataga ggaagagcaa aacaaaagta agaaaaaagc acagcaagca gcagctgaca
     4921 caggacacag caatcaggtc agccaaaatt accctatagt gcagaacatc caggggcaaa
     4981 tggtacatca ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga
     5041 aggctttcag cccagaagtg atacccatgt tttcagcatt atcagaagga gccaccccac
     5101 aagatttaaa caccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa
     5161 aagagaccat caatgaggaa gctgcagaat gggatagagt gcatccagtg catgcagggc
     5221 ctattgcacc aggccagatg agagaaccaa ggggaagtga catagcagga actactagta
     5281 cccttcagga acaaatagga tggatgacac ataatccacc tatcccagta ggagaaatct
     5341 ataaaagatg gataatcctg ggattaaata aaatagtaag aatgtatagc cctaccagca
     5401 ttctggacat aagacaagga ccaaaggaac cctttagaga ctatgtagac cgattctata
     5461 aaactctaag agccgagcaa gcttcacaag aggtaaaaaa ttggatgaca gaaaccttgt
     5521 tggtccaaaa tgcgaaccca gattgtaaga ctattttaaa agcattggga ccaggagcga
     5581 cactagaaga aatgatgaca gcatgtcagg gagtgggggg acccggccat aaagcaagag
     5641 ttttggctga agcaatgagc caagtaacaa atccagctac cataatgata cagaaaggca
     5701 attttaggaa ccaaagaaag actgttaagt gtttcaattg tggcaaagaa gggcacatag
     5761 ccaaaaattg cagggcccct aggaaaaagg gctgttggaa atgtggaaag gaaggacacc
     5821 aaatgaaaga ttgtactgag agacaggcta attttttagg gaagatctgg ccttcccaca
     5881 agggaaggcc agggaatttt cttcagagca gaccagagcc aacagcccca ccagaagaga
     5941 gcttcaggtt tggggaagag acaacaactc cctctcagaa gcaggagccg atagacaagg
     6001 aactgtatcc tttagcttcc ctcagatcac tctttggcag cgacccctcg tcacaataaa
     6061 gatagggggg caattaaagg aagctctatt agatacagga gcagatgata cagtattaga
     6121 agaaatgaat ttgccaggaa gatggaaacc aaaaatgata gggggaattg gaggttttat
     6181 caaagtaaga cagtatgatc agatactcat agaaatctgc ggacataaag ctataggtac
     6241 agtattagta ggacctacac ctgtcaacat aattggaaga aatctgttga ctcagattgg
     6301 ctgcacttta aattttccca ttagtcctat tgagactgta ccagtaaaat taaagccagg
     6361 aatggatggc ccaaaagtta aacaatggcc attgacagaa gaaaaaataa aagcattagt
     6421 agaaatttgt acagaaatgg aaaaggaagg aaaaatttca aaaattgggc ctgaaaatcc
     6481 atacaatact ccagtatttg ccataaagaa aaaagacagt actaaatgga gaaaattagt
     6541 agatttcaga gaacttaata agagaactca agatttctgg gaagttcaat taggaatacc
     6601 acatcctgca gggttaaaac agaaaaaatc agtaacagta ctggatgtgg gcgatgcata
     6661 tttttcagtt cccttagata aagacttcag gaagtatact gcatttacca tacctagtat
     6721 aaacaatgag acaccaggga ttagatatca gtacaatgtg cttccacagg gatggaaagg
     6781 atcaccagca atattccagt gtagcatgac aaaaatctta gagcctttta gaaaacaaaa
     6841 tccagacata gtcatctatc aatacatgga tgatttgtat gtaggatctg acttagaaat
     6901 agggcagcat agaacaaaaa tagaggaact gagacaacat ctgttgaggt ggggatttac
     6961 cacaccagac aaaaaacatc agaaagaacc tccattcctt tggatgggtt atgaactcca
     7021 tcctgataaa tggacagtac agcctatagt gctgccagaa aaggacagct ggactgtcaa
     7081 tgacatacag aaattagtgg gaaaattgaa ttgggcaagt cagatttatg cagggattaa
     7141 agtaaggcaa ttatgtaaac ttcttagggg aaccaaagca ctaacagaag tagtaccact
     7201 aacagaagaa gcagagctag aactggcaga aaacagggag attctaaaag aaccggtaca
     7261 tggagtgtat tatgacccat caaaagactt aatagcagaa atacagaagc aggggcaagg
     7321 ccaatggaca tatcaaattt atcaagagcc atttaaaaat ctgaaaacag gaaagtatgc
     7381 aagaatgaag ggtgcccaca ctaatgatgt gaaacaatta acagaggcag tacaaaaaat
     7441 agccacagaa agcatagtaa tatggggaaa gactcctaaa tttaaattac ccatacaaaa
     7501 ggaaacatgg gaagcatggt ggacagagta ttggcaagcc acctggattc ctgagtggga
     7561 gtttgtcaat acccctccct tagtgaagtt atggtaccag ttagagaaag aacccataat
     7621 aggagcagaa actttctatg tagatggggc agccaatagg gaaactaaat taggaaaagc
     7681 aggatatgta actgacagag gaagacaaaa agttgtcccc ctaacggaca caacaaatca
     7741 gaagactgag ttacaagcaa ttcatctagc tttgcaggat tcgggattag aagtaaacat
     7801 agtgacagac tcacaatatg cattgggaat cattcaagca caaccagata agagtgaatc
     7861 agagttagtc agtcaaataa tagagcagtt aataaaaaag gaaaaagtct acctggcatg
     7921 ggtaccagca cacaaaggaa ttggaggaaa tgaacaagta gataaattgg tcagtgctgg
     7981 aatcaggaaa gtactatttt tagatggaat agataaggcc caagaagaac atgagaaata
     8041 tcacagtaat tggagagcaa tggctagtga ttttaaccta ccacctgtag tagcaaaaga
     8101 aatagtagcc agctgtgata aatgtcagct aaaaggggaa gccatgcatg gacaagtaga
     8161 ctgtagccca ggaatatggc agctagattg tacacattta gaaggaaaag ttatcttggt
     8221 agcagttcat gtagccagtg gatatataga agcagaagta attccagcag agacagggca
     8281 agaaacagca tacttcctct taaaattagc aggaagatgg ccagtaaaaa cagtacatac
     8341 agacaatggc agcaatttca ccagtactac agttaaggcc gcctgttggt gggcggggat
     8401 caagcaggaa tttggcattc cctacaatcc ccaaagtcaa ggagtaatag aatctatgaa
     8461 taaagaatta aagaaaatta taggacaggt aagagatcag gctgaacatc ttaagacagc
     8521 agtacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     8581 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     8641 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     8701 gaaaggacca gcaaagctcc tctggaaagg tgaaggggca gtagtaatac aagataatag
     8761 tgacataaaa gtagtgccaa gaagaaaagc aaagatcatc agggattatg gaaaacagat
     8821 ggcaggtgat gattgtgtgg caagtagaca ggatgaggat taacacatgg aaaagattag
     8881 taaaacacca tatgtatatt tcaaggaaag ctaaggactg gttttataga catcactatg
     8941 aaagtactaa tccaaaaata agttcagaag tacacatccc actaggggat gctaaattag
     9001 taataacaac atattggggt ctgcatacag gagaaagaga ctggcatttg ggtcagggag
     9061 tctccataga atggaggaaa aagagatata gcacacaagt agaccctgac ctagcagacc
     9121 aactaattca tctgcactat tttgattgtt tttcagaatc tgctataaga aataccatat
     9181 taggacgtat agttagtcct aggtgtgaat atcaagcagg acataacaag gtaggatctc
     9241 tacagtactt ggcactagca gcattaataa aaccaaaaca gataaagcca cctttgccta
     9301 gtgttaggaa actgacagag gacagatgga acaagcccca gaagaccaag ggccacagag
     9361 ggagccatac aatgaatgga cactagagct tttagaggaa cttaagagtg aagctgttag
     9421 acattttcct aggatatggc tccataactt aggacaacat atctatgaaa cttacgggga
     9481 tacttgggca ggagtggaag ccataataag aattctgcaa caactgctgt ttatccattt
     9541 cagaattggg tgtcgacata gcagaatagg cgttactcga cagaggagag caagaaatgg
     9601 agccagtaga tcctagacta gagccctgga agcatccagg aagtcagcct aaaactgctt
     9661 gtaccaattg ctattgtaaa aagtgttgct ttcattgcca agtttgtttc atgacaaaag
     9721 ccttaggcat ctcctatggc aggaagaagc ggagacagcg acgaagagct catcagaaca
     9781 gtcagactca tcaagcttct ctatcaaagc agtaagtagt acatgtaatg caacctataa
     9841 tagtagcaat agtagcatta gtagtagcaa taataatagc aatagttgtg tggtccatag
     9901 taatcataga atataggaaa atattaagac aaagaaaaat agacaggtta attgatagac
     9961 taatagaaag agcagaagac agtggcaatg agagtgaagg agaagtatca gcacttgtgg
    10021 agatgggggt ggaaatgggg caccatgctc cttgggatat tgatgatctg tagttgagcg
    10081 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
    10141 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
    10201 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
    10261 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
    10321 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    10381 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    10441 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    10501 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    10561 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    10621 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    10681 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    10741 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    10801 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
    10861 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
    10921 atccattcga ttagtgaacg gatccttggc acttatctgg gacgatctgc ggagcctgtg
    10981 cctcttcagc taccaccgct tgagagactt actcttgatt gtaacgagga ttgtggaact
    11041 tctgggacgc agggggtggg aagccctcaa atattggtgg aatctcctac aatattggag
    11101 tcaggagcta aagaatagtg ctgttagctt gctcaatgcc acagccatag cagtagctga
    11161 ggggacagat agggttatag aagtagtaca aggagcttgt agagctattc gccacatacc
    11221 tagaagaata agacagggct tggaaaggat tttgctataa gatgggtggc aagtggtcaa
    11281 aaagtagtgt gattggatgg cctactgtaa gggaaagaat gagacgagct gagccagcag
    11341 cagatggggt gggagcagta tctcgagacc tagaaaaaca tggagcaatc acaagtagca
    11401 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
    11461 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
    11521 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
    11581 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
    11641 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
    11701 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
    11761 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
    11821 gccgcctagc atttcatcac atggcccgag agctgcatcc ggagtacttc aagaactgct
    11881 gagcggccgc cccggtgaca attcttaact tgtttattgc agcttataat ggttacaaat
    11941 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    12001 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccctcgaggt catcctcatc
    12061 ctgataaact gcaaaaggct cgagactagg gacaggattg gtgacagaaa agccccatcc
    12121 ttaggcctcc tccttcctag tctcctgata ttgggtctaa cccccacctc ctgttaggca
    12181 gattccttat ctggtgacac acccccattt cctggagcca tctctctcct tgccagaacc
    12241 tctaaggttt gcttacgatg gagccagaga ggatcctggg agggagagct tggcaggggg
    12301 tgggagggaa gggggggatg cgtgacctgc ccggttctca gtggccaccc tgcgctaccc
    12361 tctcccagaa cctgagctgc tctgacgcgg ctgtctggtg cgtttcactg atcctggtgc
    12421 tgcagcttcc ttacacttcc caagaggaga agcagtttgg aaaaacaaaa tcagaataag
    12481 ttggtcctga gttctaactt tggctcttca cctttctagt ccccaattta tattgttcct
    12541 ccgtgcgtca gttttacctg tgagataagg ccagtagcca gccccgtcct ggcagggctg
    12601 tggtgaggag gggggtgtcc gtgtggaaaa ctccctttgt gagaatggtg cgtcctaggt
    12661 gttcaccagg tcgtggccgc ctctactccc tttctctttc tccatccttc tttccttaaa
    12721 gagtccccag tgctatctgg gacatattcc tccgcccaga gcagggtccc gcttccctaa
    12781 ggccctgctc tgggcttctg ggtttgagtc cttggcaagc ccaggagagg cgctcaggct
    12841 tccctgtccc ccttcctcgt ccaccatctc atgcccctgg ctctcctgcc ccttccctac
    12901 aggggttcct ggctctg
//
