LOCUS       sequence-178346-        4092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human CD52 expression plasmid with short polyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Zotova A, Pichugin A, Atemasova A, Knyazhanskaya E, Lopatukhina E, 
            Mitkin N, Holmuhamedov E, Gottikh M, Kuprash D, Filatov A, Mazurov D
  TITLE     Isolation of gene-edited cells via knock-in of short 
            glycophosphatidylinositol-anchored epitope tags.
  JOURNAL   Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.1038/s41598-019-40219-z.
  PUBMED    30816313
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-28"; volume: "9(1):3132. doi"; pages: " 
            10.1038/s41598-019-40219-z"
COMMENT     Sequence Label: pJet-CMV-CD52-bglpA
FEATURES             Location/Qualifiers
     source          1..4092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    97..118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(133..138,139..156,157..163)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     enhancer        679..1058
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1059..1262
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1213..1233
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1259..1283
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    1660..1708
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(1763..1782)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1764..1782)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2088..2106)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2174..2278
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2279..2347,2348..3139)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2497..2516)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3310..3898
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3799..3818
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4052..4069
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
       61 cgactggaaa gcaattggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      121 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggaa ttatgagcgg
      181 ataataattt cacacaggag gtttaaactt taaacatgtc aaaagagacg tcttttgtta
      241 agaatgctga ggaacttgca aagcaaaaaa tggatgctat taaccctgaa ctttcttcaa
      301 aatttaaatt tttaataaaa ttcctgtctc agtttcctga agcttgctct aaacctcgtt
      361 caaaaaaaat gcagaataaa gttggtcaag aggaacatat tgaatattta gctcgtagtt
      421 ttcatgagag tcgattgcca agaaaaccca cgccacctac aacggttcct gatgaggtgg
      481 ttagcatagt tcttaatata agttttaata tacagcctga aaatcttgag agaataaaag
      541 aagaacatcg attttccatg gcagctgaga atattgtagg agatcttcta gaaagatccc
      601 cgcggccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
      661 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      721 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      781 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      841 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      901 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      961 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     1021 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     1081 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     1141 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     1201 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     1261 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     1321 gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc
     1381 cccgtgccaa gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt
     1441 cttatgcgac ggatctgcct gcaggtcgac caccatgaag cgcttcctct tcctcctact
     1501 caccatcagc ctcctggtta tggtacagat acaaactgga ctctcaggac aaaacgacac
     1561 cagccaaacc agcagcccct cagcatccag caacataagc ggaggcattt tccttttctt
     1621 cgtggccaat gccataatcc acctcttctg cttcagttga ataaaatatc tttattttca
     1681 ttacatctgt gtgttggttt tttgtgtggt accgcatctt gctgaaaaac tcgagccatc
     1741 cggaagatct ggcggccgct ctccctatag tgagtcgtat tacgccggat ggatatggtg
     1801 ttcaggcaca agtgttaaag cagttgattt tattcactat gatgaaaaaa acaatgaatg
     1861 gaacctgctc caagttaaaa atagagataa taccgaaaac tcatcgagta gtaagattag
     1921 agataataca acaataaaaa aatggtttag aacttactca cagcgtgatg ctactaattg
     1981 ggacaatttt ccagatgaag tatcatctaa gaatttaaat gaagaagact tcagagcttt
     2041 tgttaaaaat tatttggcaa aaataatata attcggctgc aggggcggcc tcgtgatacg
     2101 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2161 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2221 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2281 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2341 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2401 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2461 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2521 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2581 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2641 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2701 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2761 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2821 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2881 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2941 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3001 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     3061 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3121 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3181 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3241 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3301 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3361 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3421 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3481 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3541 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3601 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3661 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3721 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3781 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3841 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3901 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3961 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4021 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     4081 gcgcccaata cg
//
