LOCUS       sequence-178355-        9290 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible lentiviral expression of constitutively active PHF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Pattabiraman DR, Bierie B, Kober KI, Thiru P, Krall JA, Zill C, 
            Reinhardt F, Tam WL, Weinberg RA
  TITLE     Activation of PKA leads to mesenchymal-to-epithelial transition and 
            loss of tumor-initiating ability.
  JOURNAL   Science. 2016 Mar 4;351(6277):aad3680. doi: 10.1126/science.aad3680.
  PUBMED    26941323
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-03-4"; volume: "351(6277):aad3680. doi"; pages: " 
            10.1126/science.aad3680"
COMMENT     Sequence Label: pLIX402 caPHF2
FEATURES             Location/Qualifiers
     source          1..9290
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..61
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     45..61
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     66..85
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        66..84
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    102..201
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3613..3630)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3613..3629)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3622..3644)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3693..3779
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3871..3898
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(4057..4074)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4228..4816)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4308..4327)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5290..5668)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     5615..5634
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5853..5924)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5924..6059
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6099..6220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6110..6129)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6164..6183
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             6292..6525
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             6730..6741
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7139..7159
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(7199..7207,7208..7243,7244..7246,7247..7282,
                     7283..7285,7286..7321,7322..7327,7328..7945))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7946..7999)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(8000..8596)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8080..8100)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8577..8596
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8606..9116)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8669..8687)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(9126..9143)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(9159..9185)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    9193..9217
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtgcaatgta acatcagaga ttttgagaca cgggccagag ctgccaggaa acagctatga
       61 ccatgtaata cgactcacta taggggatat cagctggatg gcaaataatg attttatttt
      121 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca attatttttt ataatgccaa
      181 ctttgtacaa aaaagcagaa gggccgtcaa ggcccaccat ggcgacggtg cccgtgtact
      241 gcgtctgccg gctcccctac gacgttaccc gcttcatgat cgagtgcgac gcctgcaagg
      301 actggttcca cggcagctgt gttggggtgg aagaggagga ggcgcccgac atcgacatat
      361 accactgccc aaactgtgag aaaacccatg ggaagtccac cttaaagaag aagcggacct
      421 ggcacaaaca cggcccgggg caagcgcctg acgtcaagcc cgtgcagaat ggcagccagc
      481 tcttcatcaa ggagctgcgg agccggacct ttcccagtgc tgaagacgtg gtggcccgtg
      541 tgccaggaag tcagctcacg ctgggctaca tggaggagca cggcttcacc gagcccatcc
      601 tcgtccctaa gaaagacggg ctgggtctgg ctgtcccggc ccccacgttc tatgtcagtg
      661 acgtcgagaa ctacgtgggg ccggaacgga gtgtggatgt gacagatgtc accaagcaga
      721 aggactgcaa gatgaagctg aaggagtttg tggactatta ctacagcacc aaccgcaagc
      781 gggtcctcaa cgtcaccaac ctcgagttct ctgacacccg aatgtccagc ttcgtggagc
      841 cacctgacat tgtaaagaaa ctgtcatggg tagaaaacta ctggccagat gatgcattgc
      901 tggccaagcc caaagtgacc aagtactgcc taatctgcgt gaaggacagt tacaccgact
      961 tccacatcga ctctgggggc gcctctgcct ggtaccacgt gctcaagggg gagaagacct
     1021 tctatctcat caggccggcc tcggccaaca tctccctgta tgagcgctgg cggtctgcct
     1081 ctaaccacag cgagatgttc tttgctgacc aggtcgacaa atgctacaag tgcatcgtca
     1141 agcagggcca gaccctcttc atcccctcag gctggatcta cgccacactc acccctgtgg
     1201 actgcctggc cttcgcggga catttcctcc acagcctgag tgtggagatg cagatgagag
     1261 catatgaagt ggaaaggagg ttgaaactgg gcagcctgac tcagtttccc aactttgaaa
     1321 ctgcgtgctg gtacatgggg aagcacctgc tggaggcatt caaaggctct cacaaatctg
     1381 ggaagcagct gccccctcat ctagtccaag gagctaaaat tctcaatggt gctttccgat
     1441 cgtggacgaa gaagcaggct ttggcagagc atgaggacga gctcccggag cacttcaaac
     1501 cttcacagct aatcaaagac ctggccaaag agatccggct cagtgagaat gcctccaaag
     1561 ccgtccgacc ggaagtgaat actgtcgcct cgtcagatga ggtgtgtgac ggggaccggg
     1621 agaaggagga gcccccgtct cccattgagg ccaccccgcc tcaatccctc ctggagaaag
     1681 tgtccaaaaa aaagactccc aaaactgtga agatgcccaa gccatccaaa atccccaagc
     1741 ccccgaagcc ccctaagccc ccaaggcccc ccaaaacgct gaagctcaaa gatggaggca
     1801 agaagaaagg gaagaagtcc cgggagtcag cctcacccac catccccaac ctggacctgc
     1861 tcgaagccca caccaaggag gcactgacca agatggagcc gcccaagaag ggcaaggcca
     1921 caaagagtgt cctgagtgtg cccaacaaag atgtggttca catgcagaat gatgtggaga
     1981 ggctggaaat tcgagagcaa accaagagca agtcagaggc caagtggaag tacaagaaca
     2041 gcaaacctga ctccttactg aagatggaag aggagcagaa gctagagaag tcgcctctag
     2101 ctggaaacaa agacaataag ttctcttttt ctttctccaa caagaaactc ctcggctcca
     2161 aggctctcag gcccccgacg agccctggtg tgttcggggc cttgcagaac ttcaaggagg
     2221 acaagcccaa gcccgtgcgg gatgagtatg agtacgtgtc ggatgacggt gagctcaaga
     2281 tcgacgagtt tcccatcagg aggaagaaaa acgccccgaa aagggacttg tccttcttgt
     2341 tggataagaa ggctgtgctg cccacgcctg tcacgaagcc aaagctggac tcggcagcgt
     2401 acaagagtga tgactcctcg gacgagggtt cgctgcacat cgacacagac accaagcccg
     2461 gccgcaatgc cagagtcaag aaggaggatg ggagctcggc agctggcatc ttggacctgc
     2521 tgcaggccag tgaggaggtt ggcgcgctgg agtacaaccc cagcagccag cccccggcct
     2581 cccccagcac acaggaagcc attcagggaa tgctgtccat ggccaacctg caggcctccg
     2641 actcctgcct gcagaccacg tggggagctg gccaggccaa ggggagctcg ctggctgccc
     2701 atggtgcccg gaagaatggg ggtggcagtg gcaagagtgc aggcaaacga ctgctgaaga
     2761 gggctgccaa gaacagtgtc gacctggacg actacgagga agagcaggac cacctggatg
     2821 cctgcttcaa ggactcagac tacgtttacc cctcactgga gtcagatgaa gacaacccca
     2881 tctttaagtc ccggtcgaag aaaaggaaag gcgatgacga cgctccctac agcccaacag
     2941 caagggtcgg cccatcggtg ccaagacagg acaggcctgt gcgtgagggt acacgggtgg
     3001 cttccatcga gaccgggctg gcggctgctg cagctaagtt gtcccagcag gaggagcaga
     3061 aaagcaagaa aaaaaaggat gccaagagga agctgactcc taacaccacc tccccttcca
     3121 cctccacctc catctctgcc ggcaccacct ccacctccac cacgccagcc tctaccaccc
     3181 cggcctccac caccccggcc tccaccagca cggccagcag ccaggcctcg caggagggca
     3241 gctcgccaga gcccccgcct gagtcgcata gcagcagcct ggcggaccat gagtacacag
     3301 ccgctggcac cttcaccggg gcccaggctg gccgcacctc ccagcccatg gcccctgggg
     3361 tctttctcac acagaggcgg cccgacgcat cgtctccaaa caacaacacc gctgccaaag
     3421 gaaaacgtac gaaaaagggc atggcgaccg ccaagcagag gcttgggaaa attttgaaaa
     3481 ttcatcggaa cgggaaacta ctcctttaat gtacaaagtt ggcattataa aaaagcattg
     3541 ctcatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgggg
     3601 cccgagctta agactggccg tcgttttaca acgtcgtgac tgggaaaaca tccatgctag
     3661 cgttaacgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcggaag
     3721 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     3781 cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg gcaggacgcc
     3841 cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat ggcctttttg
     3901 cgtttctaca aactcttcct ggctagcggt acgcgtatta attgcgttgc gctcactgcc
     3961 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4021 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4081 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4141 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4201 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4261 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4321 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4381 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4441 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4501 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4561 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4621 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     4681 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4741 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4801 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4861 cgacgcgtaa ctcacgttaa gggattttgg tcatgggtgg ctcgagggtt atttgccgac
     4921 taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga
     4981 ggccaagcga tcttcttctt gtccaagata agcccrccta tctcagcgat ctgtctattt
     5041 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5101 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5161 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5221 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5281 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5341 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5401 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5461 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5521 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5581 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5641 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5701 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5761 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5821 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5881 atttatcagg gttattgtct catgagcgga tacatatttg aaaggcctcc aaaaaagcct
     5941 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
     6001 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     6061 ggcggagtta ggggcgggat agctagagcc agacatgata agatacattg atgagtttgg
     6121 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     6181 tgctttattt gtaaccatta taagctgcaa taaacaagtt cctctcactc tctgatattc
     6241 atttctttgc aagttataaa tactgaataa taagatgaca tgaactacta ctgctagaga
     6301 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac
     6361 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc
     6421 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacaag
     6481 caaaaagcag atcttgtctt cgttgggagt gaattagccc ttccagtccc cccttttctt
     6541 ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa ggtacctcta
     6601 gtccggacgc cgaggcgaaa caggcgggga ggcggcccaa agggagatcc gactcgtctg
     6661 agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag
     6721 gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt
     6781 ggcaacacag gcgagcagcc atggaaagga cgtcagcttc cccgacaaca ccacggaatt
     6841 gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc
     6901 cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc
     6961 aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct
     7021 gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa
     7081 aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca
     7141 tagcgtaaaa ggagcaacat agttaagaat acgatatctt ggatcgatcc ttacttagtt
     7201 acccggggag catgtcaagg tcaaaatcgt caagagcgtc agcaggcagc atatcaaggt
     7261 caaagtcgtc aagggcatcg gctgggagca tgtctaagtc aaaatcgtca agggcgtcgg
     7321 tcggcccgcc gctttcgcac tttagctgtt tctccaggcc acatatgatt agttccaggc
     7381 cgaaaaggaa ggcaggttcg gctccctgcc ggtcgaacag ctcaattgct tgtctcagaa
     7441 gtgggggcat agaatcggtg gtaggtgtct ctctttcctc ttttgctact tgatgctcct
     7501 gttcctccaa tacgcagccc agtgtaaagt ggcccacggc ggacagagcg tacagtgcgt
     7561 tctccaggga gaagccttgc tgacacagga acgcgagctg attttccagg gtttcgtact
     7621 gtttctctgt tgggcgggtg ccgagatgca ctttagcccc gtcgcgatgt gagaggagag
     7681 cacagcggta tgacttggcg ttgttccgca gaaagtcttg ccatgactcg ccttccaggg
     7741 ggcagaagtg ggtatgatgc ctgtccagca tctcgattgg cagggcatcg agcagggccc
     7801 gcttgttctt cacgtgccag tacagggtag gctgctcaac tcccagcttt tgagcgagtt
     7861 tccttgtcgt caggccttcg ataccgacac cattgagtaa ttccagagct ccgtttatga
     7921 ctttgctctt gtccagtcta gacattggac cagggttttc ttcaacatca ccacaagtga
     7981 ggagagaacc tctaccttcg gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt
     8041 cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgc
     8101 ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg
     8161 ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg
     8221 ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct
     8281 gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg
     8341 cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga
     8401 cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc agctcggtga
     8461 cccgctcgat gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg
     8521 cggcggcgag ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg
     8581 gcttgtactc ggtcatggtg aattgctggg gagagaggtc ggtgattcgg tcaacgaggg
     8641 agccgactgc cgacgtgcgc tccggaggct tgcagaatgc ggaacaccgc gcgggcagga
     8701 acagggccca cactaccgcc ccacaccccg cctcccgcac cgccccttcc cggccgctgc
     8761 tctcggcgcg ccctgctgag cagccgctat tggccacagc ccatcgcggt cggcgcgctg
     8821 ccattgctcc ctggcgctgt ccgtctgcga gggtactagt gagacgtgcg gcttccgttt
     8881 gtcacgtccg gcacgccgcg aaccgcaagg aaccttcccg acttaggggc ggagcaggaa
     8941 gcgtcgccgg ggggcccaca agggtagcgg cgaagatccg ggtgacgctg cgaacggacg
     9001 tgaagaatgt gcgagaccca gggtcggcgc cgctgcgttt cccggaacca cgcccagagc
     9061 agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag gcgcaacccc aaccccggat
     9121 ccttagtggt ggtggtggtg gtggaccgga cgcgtttacg cataatccgg cacatcatac
     9181 ggataaccgg taaccacttt gtacaagaaa gctgaacgag aaacgtaaaa tgatataaat
     9241 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
//
