LOCUS       sequence-178361-       11414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11414)
  AUTHORS   Ledoux S, Guthrie C
  TITLE     Retinitis Pigmentosa Mutations in Bad Response to Refrigeration 2 
            (Brr2) Impair ATPase and Helicase Activity.
  JOURNAL   J Biol Chem. 2016 Jun 3;291(23):11954-65. doi: 
            10.1074/jbc.M115.710848. Epub 2016 Apr 12.
  PUBMED    27072132
REFERENCE   2  (bases 1 to 11414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.710848"; journalName: "J Biol Chem"; date: 
            "2016-06-3- 3"; volume: "291"; issue: "23"; pages: "11954-65"
COMMENT     Sequence Label: pSL11 - Brr2 del422 N1104L
FEATURES             Location/Qualifiers
     source          1..11414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..645
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     538..561
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             5900..5977
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             6005..6025
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             6065..6238
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             6239..6412
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     primer_bind     complement(6476..6492)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6476..6492)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6518..6537)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6519..6537)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6547..6564)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6547..6563)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6556..6578)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6704..7159
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6791..6810)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7001..7022
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7419..8081)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(8082..8270)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(8417..8436)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8536..8558
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8596..8614)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    8662..9165
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        9195..9299
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9300..9368,9369..10160)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9518..9537)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10331..10919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10820..10839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11073..11090
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11207..11228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11243..11248,11249..11266,11267..11273)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11281..11297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11286..11308
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11305..11321
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11305..11321
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11340..11360
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11342..11360
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcattatcaa tactgccatt tcaaagaata cgtaaataat taatagtagt gattttccta
       61 actttattta gtcaaaaaat tagcctttta attctgctgt aacccgtaca tgcccaaaat
      121 agggggcggg ttacacagaa tatataacat cgtaggtgtc tgggtgaaca gtttattcct
      181 ggcatccact aaatataatg gagcccgctt tttaagctgg catccagaaa aaaaaaagaa
      241 tcccagcacc aaaatattgt tttcttcacc aaccatcagt tcataggtcc attctcttag
      301 cgcaactaca gagaacaggg gcacaaacag gcaaaaaacg ggcacaacct caatggagtg
      361 atgcaacctg cctggagtaa atgatgacac aaggcaattg acccacgcat gtatctatct
      421 cattttctta caccttctat taccttctgc tctctctgat tcggaaaaag ctgaaaaaaa
      481 aggttgaaac cagttccctg aaattactcc cctacttgac taataagtat ataaagacgg
      541 taggtattga ttgtaattct gtaaatctat ttcttaaact tcttaaattc tacttttata
      601 gttagtcttt tttttagttt taaaacacca agaacttagt ttcgactcta gaatggcaat
      661 accgcccgtc atagatttgg aaaagattaa attcgacgaa agttctaaat taatgaccgt
      721 gacaaaagta tccctaccgg aaggatcatt caaaagggtg aaaccacaat atgacgaaat
      781 acatattcca gctcctagca aacctgttat tgattatgag ttgaaagaaa ttacttctct
      841 accagattgg tgtcaggagg cgtttccttc atcggaaaca acgtccttaa accctataca
      901 atcaaaggtt tttcacgcag catttgaagg tgattcaaac atgctaatat gtgcacccac
      961 tggttcaggt aagacgaata tcgcgttatt gacggtattg aaagccttat cccatcatta
     1021 taacccgaaa actaagaaat taaacctttc tgcatttaaa attgtttata tagcaccctt
     1081 gaaagcctta gtacaggaac aagtccgaga atttcaaagg aggttggctt ttctaggtat
     1141 aaaagtcgct gaattgactg gtgattctag gcttagtaga aagcaaattg acgagacgca
     1201 ggttttggtg tctacgccag agaaatggga cataactact agaaattcaa ataatttggc
     1261 catcgtagaa cttgttcgtt tgttgattat agatgaaata catttgttac atgatgacag
     1321 gggaccagta ttggaaagca tcgtggctag gacattttgg gcttccaaat atggccagga
     1381 ataccccaga ataataggcc tttctgccac cttaccaaat tatgaggatg tcggtaggtt
     1441 tttaagagtt ccgaaggaag gattgtttta ttttgactcc tcatttagac cgtgtcctct
     1501 atctcaacag ttttgtggca ttaaggaacg gaactccttg aaaaagctga aggctatgaa
     1561 tgatgcttgt tatgaaaagg tattggaatc catcaatgaa ggtaatcaaa taatcgtgtt
     1621 tgttcattct cgaaaagaaa catcacgaac agcaacatgg ttgaaaaata aatttgctga
     1681 agagaacatt actcataaat taaccaaaaa tgatgcagga tcgaagcaga ttttaaagac
     1741 agaagcagcc aacgtactcg atccaagtct acgaaagttg attgaaagcg gtatagggac
     1801 gcatcacgca gggctgacca gaagtgacag atccttatct gaagatttat ttgcagacgg
     1861 gttgttgcaa gtattggtat gcactgcgac gttagcatgg ggtgttaatc tacctgctca
     1921 tacagtgatt ataaaaggaa cagatgtcta ttcacctgaa aaggggtcct gggaacaact
     1981 ttctccacaa gatgtacttc aaatgttagg tagagcaggc agaccgagat atgacacatt
     2041 tggcgagggt attataataa cagaccaatc gaatgttcaa tattatctct cggttttaaa
     2101 tcaacaatta ccaatagaat cgcaatttgt ttcaaaatta gttgacaact taaatgcaga
     2161 agttgtcgcg ggtaacatta agtgcaggaa tgatgctgtg aattggttgg cgtatactta
     2221 cctatatgtc agaatgttag cttcccctat gctttacaaa gtacctgata tttcaagtga
     2281 tgggcaattg aaaaagttta gagaaagctt agtccattcg gccctatgca ttttaaaaga
     2341 acaagagttg gtgttatatg atgctgaaaa tgatgtaatt gaggcgaccg atctaggaaa
     2401 tatagcgtcg tcattttaca taaatcatgc ttccatggat gtttacaata gagagttaga
     2461 tgaacataca actcagattg atctttttag aatattctca atgtcggaag agtttaagta
     2521 cgtatcggtg agatatgaag agaaaagaga actaaaacaa cttctagaga aagcacccat
     2581 tcctatcaga gaagatattg atgatccttt agcaaaagtg aatgtactac tgcagtctta
     2641 tttttcccag ctaaagttcg aaggttttgc acttaattct gatatagtgt ttattcacca
     2701 attggctggt aggttattgc gtgctatgtt tgaaatatgc ttgaagagag gatggggaca
     2761 tcccacacgg atgttgttaa atctttgcaa atctgccaca actaaaatgt ggccaacaaa
     2821 ctgtccttta agacagttta aaacatgccc tgtcgaagtg attaaaaggc tggaagcctc
     2881 tacagtaccg tggggagatt atctacagct agaaacacct gccgaagttg gtcgtgcaat
     2941 cagatctgaa aaatatggaa aacaggttta cgatttattg aaaagatttc ccaaaatgtc
     3001 cgtaacatgc aacgctcagc caattacccg tagcgttatg aggttcaaca ttgaaataat
     3061 agccgactgg atatgggata tgaatgtaca cggttccctt gaaccttttt tgctcatgct
     3121 agaagacact gatggtgatt caatacttta ttatgacgta ctattcatca ctccagatat
     3181 agttggtcat gaatttacct tatcctttac ttatgagtta aagcagcaca atcaaaataa
     3241 cttaccacct aatttcttcc ttactttgat atcagaaaat tggtggcaca gtgaattcga
     3301 gattccagtt tccttcaatg gatttaaact tccgaaaaaa tttcctcctc ctactcccct
     3361 tctggagaat attagtattt caacatcaga gcttggcaat gatgattttt ccgaagtatt
     3421 tgaatttaaa actttcaaca agattcagag ccaggtcttc gagtctctct acaattcgaa
     3481 tgactcggtc tttgtaggtt caggaaaggg cacaggtaaa acagctatgg ctgaattagc
     3541 actgttaaat cattggagac agaataaagg aagagcggtt tatatcaatc cgtctgggga
     3601 aaaaattgat tttttgctat ctgattggaa taaaaggttc tctcatcttg ctggtggtaa
     3661 gataataaat aagctaggaa atgatccttc actaaattta aaattattag caaaaagcca
     3721 tgttctcttg gctacccccg tgcaatttga gcttttgtca cgtcgttgga gacaaaggaa
     3781 gaacatccaa agtttagaat taatgatata tgatgacgct catgagatta gccaaggtgt
     3841 ttacggtgct gtgtatgaaa cgctaatttc tagaatgata tttatagcaa cccaacttga
     3901 aaagaaaatt cgatttgttt gcctttcgaa ctgtttggca aatgctcgtg attttggtga
     3961 atgggctggt atgacgaagt caaacatcta caacttttct ccaagcgaaa gaattgagcc
     4021 tcttgaaatt aatattcagt catttaaaga tgttgagcac atatcattca atttttcgat
     4081 gcttcaaatg gcatttgaag catctgctgc tgcagcggga aatcgtaatt ccagttcggt
     4141 gttcttacca tctagaaagg attgtatgga agttgcgtct gcatttatga agttttcaaa
     4201 agcaattgaa tgggatatgt tgaacgttga agaagagcaa attgtaccat acattgaaaa
     4261 gttaacagat ggtcatctaa gagcaccttt aaaacacggt gttggtattt tgtataaggg
     4321 gatggcatca aatgatgaaa gaattgtgaa aaggttatac gaatatggtg cggtatcggt
     4381 tttactcatt tctaaggatt gttctgcatt cgcttgtaaa actgatgaag tgattatttt
     4441 aggaaccaac ctttatgatg gtgcagagca taaatatatg ccatatacca taaatgaact
     4501 tctagaaatg gtgggactag ctagtggtaa cgattcaatg gcaggcaagg ttttaatttt
     4561 gacaagtcac aacatgaagg cctactacaa aaaattctta attgagcctt tgcctactga
     4621 gagttaccta cagtatatta ttcatgatac tctgaacaac gagatagcaa attctattat
     4681 acaaagcaaa caagattgtg ttgactggtt tacatattcg tatttctatc gtcgtatcca
     4741 tgtgaatcca agctattatg gcgtaaggga tacgtctcca catggaattt cagttttttt
     4801 gagtaattta gttgaaactt gtttgaatga cttagttgaa tcatccttca ttgaaattga
     4861 cgatacagaa gctgaagtaa ccgcagaagt taatggagga gatgatgaag caactgaaat
     4921 catttctacc ttgagcaacg ggctaattgc ttctcactat ggcgtatcat tttttactat
     4981 tcagtcattc gtttcttctt tatcgaatac gtcaacgctc aaaaacatgc tgtatgttct
     5041 ttcaacagca gtagaattcg aaagtgttcc actcagaaaa ggagacagag cattacttgt
     5101 caaattaagt aaaagactac cattgagatt ccctgaacac acctcttctg gttcggtgag
     5161 ctttaaagtt ttcttattgt tacaagcata tttttcacgt cttgaattac ctgtcgactt
     5221 tcaaaatgat ttaaaagaca ttctagaaaa ggttgttcca ctaattaacg tagtggttga
     5281 tatcctttca gcaaacgggt atttgaatgc tactaccgcc atggatttgg cgcagatgtt
     5341 gattcagggt gtatgggacg ttgataatcc gttgagacag attccacact tcaacaacaa
     5401 aattttggaa aaatgtaaag aaattaacgt cgagacggtc tatgatataa tggcccttga
     5461 ggatgaagag agggatgaaa ttttaacgct tactgattca cagcttgcgc aggtcgccgc
     5521 gtttgttaac aattacccca acgttgaact tacttattcg ttgaataatt ctgattcgtt
     5581 aatttcaggt gtaaaacaga aaattaccat tcaattgaca agagatgttg aacctgagaa
     5641 cttacaagta acatctgaaa agtatccgtt tgataagcta gaaagttggt ggttggtttt
     5701 aggtgaagta tctaaaaaag agctttatgc gatcaagaaa gttactttaa ataaagagac
     5761 tcagcaatat gaattggaat ttgacactcc gacatctggt aaacataact taacaatttg
     5821 gtgtgtctgt gattcatatc ttgacgcaga taaagagttg tcctttgaaa taaatgtgaa
     5881 actcgaggga tccatggaaa agagaagatg gaaaaagaat ttcatagccg tctcagcagc
     5941 caaccgcttt aagaaaatct catcctccgg ggcacttgat tatgatattc caactactgc
     6001 tagcgagaat ttgtattttc agggtgagct caaaaccgcg gctcttgcgc aacacgatga
     6061 agccgtggac aacaaattca acaaagaaca acaaaacgcg ttctatgaga tcttacattt
     6121 acctaactta aacgaagaac aacgaaacgc cttcatccaa agtttaaaag atgacccaag
     6181 ccaaagcgct aaccttttag cagaagctaa aaagctaaat gatgctcagg cgccgaaagt
     6241 agacaacaaa ttcaacaaag agcaacaaaa cgcgttctat gagatcttac atttacctaa
     6301 cttaaacgaa gaacaacgaa acgccttcat ccaaagttta aaagatgacc caagccaaag
     6361 cgctaacctt ttagcagaag ctaaaaagct aaatggtgct caggcgccga aagtagacgc
     6421 gaattccgcg gggaagtcaa cctgaagctt gatatcgaat tcctgtagcc cgggggatcc
     6481 actagttcta gagcggccgg gcccggtacc caattcgccc tatagtgagt cgtattacgc
     6541 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     6601 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     6661 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc
     6721 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6781 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6841 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6901 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6961 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7021 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7081 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7141 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     7201 cacaccgcat agatccgtcg agttcaagag aaaaaaaaag aaaaagcaaa aagaaaaaag
     7261 gaaagcgcgc ctcgttcaga atgacacgta tagaatgatg cattaccttg tcatcttcag
     7321 tatcatactg ttcgtataca tacttactga cattcatagg tatacatata tacacatgta
     7381 tatatatcgt atgctgcagc tttaaataat cggtgtcact acataagaac acctttggtg
     7441 gagggaacat cgttggtacc attgggcgag gtggcttctc ttatggcaac cgcaagagcc
     7501 ttgaacgcac tctcactacg gtgatgatca ttcttgcctc gcagacaatc aacgtggagg
     7561 gtaattctgc tagcctctgc aaagctttca agaaaatgcg ggatcatctc gcaagagaga
     7621 tctcctactt tctccctttg caaaccaagt tcgacaactg cgtacggcct gttcgaaaga
     7681 tctaccaccg ctctggaaag tgcctcatcc aaaggcgcaa atcctgatcc aaaccttttt
     7741 actccacgca cggcccctag ggcctcttta aaagcttgac cgagagcaat cccgcagtct
     7801 tcagtggtgt gatggtcgtc tatgtgtaag tcaccaatgc actcaacgat tagcgaccag
     7861 ccggaatgct tggccagagc atgtatcata tggtccagaa accctatacc tgtgtggacg
     7921 ttaatcactt gcgattgtgt ggcctgttct gctactgctt ctgcctcttt ttctgggaag
     7981 atcgagtgct ctatcgctag gggaccaccc tttaaagaga tcgcaatctg aatcttggtt
     8041 tcatttgtaa tacgctttac tagggctttc tgctctgtca tctttgcctt cgtttatctt
     8101 gcctgctcat tttttagtat attcttcgaa gaaatcacat tactttatat aatgtataat
     8161 tcattatgtg ataatgccaa tcgctaagaa aaaaaaagag tcatccgcta ggtggaaaaa
     8221 aaaaaaatga aaatcattac cgaggcataa aaaaatatag agtgtactag aggaggccaa
     8281 gagtaataga aaaagaaaat tgcgggaaag gactgtgtta tgacttccct gactaatgcc
     8341 gtgttcaaac gatacctggc agtgactcct agcgctcacc aagctcttaa aacgggaatt
     8401 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     8461 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     8521 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     8581 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     8641 tggtttctta gggtcctttt catcacgtgc tataaaaata attataattt aaatttttta
     8701 atataaatat ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt
     8761 tgttttccga agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt
     8821 gctcttcctg ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca
     8881 cacacgaaaa tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta
     8941 atctgctttt cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta
     9001 aacctttgtt tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc
     9061 taaaatccaa atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc
     9121 atcattaaaa gatacgaggc gcgtgtaagt tacaggcaag cgatccgtca ggtggcactt
     9181 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     9241 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     9301 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9361 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9421 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9481 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9541 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9601 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9661 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9721 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9781 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9841 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9901 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9961 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    10021 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    10081 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    10141 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    10201 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    10261 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10321 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10381 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10441 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    10501 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10561 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10621 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10681 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    10741 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10801 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10861 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10921 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10981 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    11041 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    11101 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    11161 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    11221 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    11281 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    11341 caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgcggct
    11401 gcaggtcgag ttta
//
