LOCUS       sequence-178362-       11412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11412)
  AUTHORS   Ledoux S, Guthrie C
  TITLE     Retinitis Pigmentosa Mutations in Bad Response to Refrigeration 2 
            (Brr2) Impair ATPase and Helicase Activity.
  JOURNAL   J Biol Chem. 2016 Jun 3;291(23):11954-65. doi: 
            10.1074/jbc.M115.710848. Epub 2016 Apr 12.
  PUBMED    27072132
REFERENCE   2  (bases 1 to 11412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.710848"; journalName: "J Biol Chem"; date: 
            "2016-06-3- 3"; volume: "291"; issue: "23"; pages: "11954-65"
COMMENT     Sequence Label: pSL12 - Brr2 del422 Q904E
FEATURES             Location/Qualifiers
     source          1..11412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..644
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     537..560
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             5899..5976
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             6004..6024
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             6064..6237
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             6238..6411
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     primer_bind     complement(6475..6491)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6475..6491)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6517..6536)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6518..6536)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6546..6563)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6546..6562)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6555..6577)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6703..7158
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6790..6809)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7000..7021
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7418..8080)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(8081..8268)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(8415..8434)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8534..8556
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8594..8612)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    8660..9163
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        9193..9297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9298..9366,9367..10158)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9516..9535)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10329..10917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10818..10837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11071..11088
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11205..11226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11241..11246,11247..11264,11265..11271)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11279..11295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11284..11306
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11303..11319
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11303..11319
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11338..11358
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11340..11358
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcattatcaa tactgccatt tcaaagaata cgtaaataat taatagtagt gattttccta
       61 actttattta gtcaaaaaat tagcctttta attctgctgt aacccgtaca tgcccaaaat
      121 agggggcggg ttacacagaa tatataacat cgtaggtgtc tgggtgaaca gtttattcct
      181 ggcatccact aaatataatg gagcccgctt tttaagctgg catccagaaa aaaaaagaat
      241 cccagcacca aaatattgtt ttcttcacca accatcagtt cataggtcca ttctcttagc
      301 gcaactacag agaacagggg cacaaacagg caaaaaacgg gcacaacctc aatggagtga
      361 tgcaacctgc ctggagtaaa tgatgacaca aggcaattga cccacgcatg tatctatctc
      421 attttcttac accttctatt accttctgct ctctctgatt cggaaaaagc tgaaaaaaaa
      481 ggttgaaacc agttccctga aattactccc ctacttgact aataagtata taaagacggt
      541 aggtattgat tgtaattctg taaatctatt tcttaaactt cttaaattct acttttatag
      601 ttagtctttt ttttagtttt aaaacaccaa gaacttagtt tcgactctag aatggcaata
      661 ccgcccgtca tagatttgga aaagattaaa ttcgacgaaa gttctaaatt aatgaccgtg
      721 acaaaagtat ccctaccgga aggatcattc aaaagggtga aaccacaata tgacgaaata
      781 catattccag ctcctagcaa acctgttatt gattatgagt tgaaagaaat tacttctcta
      841 ccagattggt gtcaggaggc gtttccttca tcggaaacaa cgtccttaaa ccctatacaa
      901 tcaaaggttt ttcacgcagc atttgaaggt gattcaaaca tgctaatatg tgcacccact
      961 ggttcaggta agacgaatat cgcgttattg acggtattga aagccttatc ccatcattat
     1021 aacccgaaaa ctaagaaatt aaacctttct gcatttaaaa ttgtttatat agcacccttg
     1081 aaagccttag tacaggaaca agtccgagaa tttcaaagga ggttggcttt tctaggtata
     1141 aaagtcgctg aattgactgg tgattctagg cttagtagaa agcaaattga cgagacgcag
     1201 gttttggtgt ctacgccaga gaaatgggac ataactacta gaaattcaaa taatttggcc
     1261 atcgtagaac ttgttcgttt gttgattata gatgaaatac atttgttaca tgatgacagg
     1321 ggaccagtat tggaaagcat cgtggctagg acattttggg cttccaaata tggccaggaa
     1381 taccccagaa taataggcct ttctgccacc ttaccaaatt atgaggatgt cggtaggttt
     1441 ttaagagttc cgaaggaagg attgttttat tttgactcct catttagacc gtgtcctcta
     1501 tctcaacagt tttgtggcat taaggaacgg aactccttga aaaagctgaa ggctatgaat
     1561 gatgcttgtt atgaaaaggt attggaatcc atcaatgaag gtaatcaaat aatcgtgttt
     1621 gttcattctc gaaaagaaac atcacgaaca gcaacatggt tgaaaaataa atttgctgaa
     1681 gagaacatta ctcataaatt aaccaaaaat gatgcaggat cgaagcagat tttaaagaca
     1741 gaagcagcca acgtactcga tccaagtcta cgaaagttga ttgaaagcgg tatagggacg
     1801 catcacgcag ggctgaccag aagtgacaga tccttatctg aagatttatt tgcagacggg
     1861 ttgttgcaag tattggtatg cactgcgacg ttagcatggg gtgttaatct acctgctcat
     1921 acagtgatta taaaaggaac agatgtctat tcacctgaaa aggggtcctg ggaacaactt
     1981 tctccacaag atgtacttca aatgttaggt agagcaggca gaccgagata tgacacattt
     2041 ggcgagggta ttataataac agaccaatcg aatgttcaat attatctctc ggttttaaat
     2101 gaacaattac caatagaatc gcaatttgtt tcaaaattag ttgacaactt aaatgcagaa
     2161 gttgtcgcgg gtaacattaa gtgcaggaat gatgctgtga attggttggc gtatacttac
     2221 ctatatgtca gaatgttagc ttcccctatg ctttacaaag tacctgatat ttcaagtgat
     2281 gggcaattga aaaagtttag agaaagctta gtccattcgg ccctatgcat tttaaaagaa
     2341 caagagttgg tgttatatga tgctgaaaat gatgtaattg aggcgaccga tctaggaaat
     2401 atagcgtcgt cattttacat aaatcatgct tccatggatg tttacaatag agagttagat
     2461 gaacatacaa ctcagattga tctttttaga atattctcaa tgtcggaaga gtttaagtac
     2521 gtatcggtga gatatgaaga gaaaagagaa ctaaaacaac ttctagagaa agcacccatt
     2581 cctatcagag aagatattga tgatccttta gcaaaagtga atgtactact gcagtcttat
     2641 ttttcccagc taaagttcga aggttttgca cttaattctg atatagtgtt tattcaccaa
     2701 aatgctggta ggttattgcg tgctatgttt gaaatatgct tgaagagagg atggggacat
     2761 cccacacgga tgttgttaaa tctttgcaaa tctgccacaa ctaaaatgtg gccaacaaac
     2821 tgtcctttaa gacagtttaa aacatgccct gtcgaagtga ttaaaaggct ggaagcctct
     2881 acagtaccgt ggggagatta tctacagcta gaaacacctg ccgaagttgg tcgtgcaatc
     2941 agatctgaaa aatatggaaa acaggtttac gatttattga aaagatttcc caaaatgtcc
     3001 gtaacatgca acgctcagcc aattacccgt agcgttatga ggttcaacat tgaaataata
     3061 gccgactgga tatgggatat gaatgtacac ggttcccttg aacctttttt gctcatgcta
     3121 gaagacactg atggtgattc aatactttat tatgacgtac tattcatcac tccagatata
     3181 gttggtcatg aatttacctt atcctttact tatgagttaa agcagcacaa tcaaaataac
     3241 ttaccaccta atttcttcct tactttgata tcagaaaatt ggtggcacag tgaattcgag
     3301 attccagttt ccttcaatgg atttaaactt ccgaaaaaat ttcctcctcc tactcccctt
     3361 ctggagaata ttagtatttc aacatcagag cttggcaatg atgatttttc cgaagtattt
     3421 gaatttaaaa ctttcaacaa gattcagagc caggtcttcg agtctctcta caattcgaat
     3481 gactcggtct ttgtaggttc aggaaagggc acaggtaaaa cagctatggc tgaattagca
     3541 ctgttaaatc attggagaca gaataaagga agagcggttt atatcaatcc gtctggggaa
     3601 aaaattgatt ttttgctatc tgattggaat aaaaggttct ctcatcttgc tggtggtaag
     3661 ataataaata agctaggaaa tgatccttca ctaaatttaa aattattagc aaaaagccat
     3721 gttctcttgg ctacccccgt gcaatttgag cttttgtcac gtcgttggag acaaaggaag
     3781 aacatccaaa gtttagaatt aatgatatat gatgacgctc atgagattag ccaaggtgtt
     3841 tacggtgctg tgtatgaaac gctaatttct agaatgatat ttatagcaac ccaacttgaa
     3901 aagaaaattc gatttgtttg cctttcgaac tgtttggcaa atgctcgtga ttttggtgaa
     3961 tgggctggta tgacgaagtc aaacatctac aacttttctc caagcgaaag aattgagcct
     4021 cttgaaatta atattcagtc atttaaagat gttgagcaca tatcattcaa tttttcgatg
     4081 cttcaaatgg catttgaagc atctgctgct gcagcgggaa atcgtaattc cagttcggtg
     4141 ttcttaccat ctagaaagga ttgtatggaa gttgcgtctg catttatgaa gttttcaaaa
     4201 gcaattgaat gggatatgtt gaacgttgaa gaagagcaaa ttgtaccata cattgaaaag
     4261 ttaacagatg gtcatctaag agcaccttta aaacacggtg ttggtatttt gtataagggg
     4321 atggcatcaa atgatgaaag aattgtgaaa aggttatacg aatatggtgc ggtatcggtt
     4381 ttactcattt ctaaggattg ttctgcattc gcttgtaaaa ctgatgaagt gattatttta
     4441 ggaaccaacc tttatgatgg tgcagagcat aaatatatgc catataccat aaatgaactt
     4501 ctagaaatgg tgggactagc tagtggtaac gattcaatgg caggcaaggt tttaattttg
     4561 acaagtcaca acatgaaggc ctactacaaa aaattcttaa ttgagccttt gcctactgag
     4621 agttacctac agtatattat tcatgatact ctgaacaacg agatagcaaa ttctattata
     4681 caaagcaaac aagattgtgt tgactggttt acatattcgt atttctatcg tcgtatccat
     4741 gtgaatccaa gctattatgg cgtaagggat acgtctccac atggaatttc agtttttttg
     4801 agtaatttag ttgaaacttg tttgaatgac ttagttgaat catccttcat tgaaattgac
     4861 gatacagaag ctgaagtaac cgcagaagtt aatggaggag atgatgaagc aactgaaatc
     4921 atttctacct tgagcaacgg gctaattgct tctcactatg gcgtatcatt ttttactatt
     4981 cagtcattcg tttcttcttt atcgaatacg tcaacgctca aaaacatgct gtatgttctt
     5041 tcaacagcag tagaattcga aagtgttcca ctcagaaaag gagacagagc attacttgtc
     5101 aaattaagta aaagactacc attgagattc cctgaacaca cctcttctgg ttcggtgagc
     5161 tttaaagttt tcttattgtt acaagcatat ttttcacgtc ttgaattacc tgtcgacttt
     5221 caaaatgatt taaaagacat tctagaaaag gttgttccac taattaacgt agtggttgat
     5281 atcctttcag caaacgggta tttgaatgct actaccgcca tggatttggc gcagatgttg
     5341 attcagggtg tatgggacgt tgataatccg ttgagacaga ttccacactt caacaacaaa
     5401 attttggaaa aatgtaaaga aattaacgtc gagacggtct atgatataat ggcccttgag
     5461 gatgaagaga gggatgaaat tttaacgctt actgattcac agcttgcgca ggtcgccgcg
     5521 tttgttaaca attaccccaa cgttgaactt acttattcgt tgaataattc tgattcgtta
     5581 atttcaggtg taaaacagaa aattaccatt caattgacaa gagatgttga acctgagaac
     5641 ttacaagtaa catctgaaaa gtatccgttt gataagctag aaagttggtg gttggtttta
     5701 ggtgaagtat ctaaaaaaga gctttatgcg atcaagaaag ttactttaaa taaagagact
     5761 cagcaatatg aattggaatt tgacactccg acatctggta aacataactt aacaatttgg
     5821 tgtgtctgtg attcatatct tgacgcagat aaagagttgt cctttgaaat aaatgtgaaa
     5881 ctcgagggat ccatggaaaa gagaagatgg aaaaagaatt tcatagccgt ctcagcagcc
     5941 aaccgcttta agaaaatctc atcctccggg gcacttgatt atgatattcc aactactgct
     6001 agcgagaatt tgtattttca gggtgaactc aaaaccgcgg ctcttgcgca acacgatgaa
     6061 gccgtggaca acaaattcaa caaagaacaa caaaacgcgt tctatgagat cttacattta
     6121 cctaacttaa acgaagaaca acgaaacgcc ttcatccaaa gtttaaaaga tgacccaagc
     6181 caaagcgcta accttttagc agaagctaaa aagctaaatg atgctcaggc gccgaaagta
     6241 gacaacaaat tcaacaaaga gcaacaaaac gcgttctatg agatcttaca tttacctaac
     6301 ttaaacgaag aacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc
     6361 gctaaccttt tagcagaagc taaaaagcta aatggtgctc aggcgccgaa agtagacgcg
     6421 aattccgcgg ggaagtcaac ctgaagcttg atatcgaatt cctgtagccc gggggatcca
     6481 ctagttctag agcggccggg cccggtaccc aattcgccct atagtgagtc gtattacgcg
     6541 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6601 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6661 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
     6721 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     6781 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     6841 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     6901 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6961 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     7021 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     7081 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     7141 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     7201 acaccgcata gatccgtcga gttcaagaga aaaaaaaaga aaaagcaaaa agaaaaaagg
     7261 aaagcgcgcc tcgttcagaa tgacacgtat agaatgatgc attaccttgt catcttcagt
     7321 atcatactgt tcgtatacat acttactgac attcataggt atacatatat acacatgtat
     7381 atatatcgta tgctgcagct ttaaataatc ggtgtcacta cataagaaca cctttggtgg
     7441 agggaacatc gttggtacca ttgggcgagg tggcttctct tatggcaacc gcaagagcct
     7501 tgaacgcact ctcactacgg tgatgatcat tcttgcctcg cagacaatca acgtggaggg
     7561 taattctgct agcctctgca aagctttcaa gaaaatgcgg gatcatctcg caagagagat
     7621 ctcctacttt ctccctttgc aaaccaagtt cgacaactgc gtacggcctg ttcgaaagat
     7681 ctaccaccgc tctggaaagt gcctcatcca aaggcgcaaa tcctgatcca aaccttttta
     7741 ctccacgcac ggcccctagg gcctctttaa aagcttgacc gagagcaatc ccgcagtctt
     7801 cagtggtgtg atggtcgtct atgtgtaagt caccaatgca ctcaacgatt agcgaccagc
     7861 cggaatgctt ggccagagca tgtatcatat ggtccagaaa ccctatacct gtgtggacgt
     7921 taatcacttg cgattgtgtg gcctgttctg ctactgcttc tgcctctttt tctgggaaga
     7981 tcgagtgctc tatcgctagg ggaccaccct ttaaagagat cgcaatctga atcttggttt
     8041 catttgtaat acgctttact agggctttct gctctgtcat ctttgccttc gtttatcttg
     8101 cctgctcatt ttttagtata ttcttcgaag aaatcacatt actttatata atgtataatt
     8161 cattatgtga taatgccaat cgctaagaaa aaaaaagagt catccgctag gtggaaaaaa
     8221 aaaaatgaaa atcattaccg aggcataaaa aaatatagag tgtactagag gaggccaaga
     8281 gtaatagaaa aagaaaattg cgggaaagga ctgtgttatg acttccctga ctaatgccgt
     8341 gttcaaacga tacctggcag tgactcctag cgctcaccaa gctcttaaaa cgggaattta
     8401 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     8461 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     8521 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     8581 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     8641 gtttcttagg gtccttttca tcacgtgcta taaaaataat tataatttaa attttttaat
     8701 ataaatatat aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg
     8761 ttttccgaag atgtaaaaga ctctaggggg atcgccaaca aatactacct tttatcttgc
     8821 tcttcctgct ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta gaagaccaca
     8881 cacgaaaatc ctgtgatttt acattttact tatcgttaat cgaatgtata tctatttaat
     8941 ctgcttttct tgtctaataa atatatatgt aaagtacgct ttttgttgaa attttttaaa
     9001 cctttgttta tttttttttc ttcattccgt aactcttcta ccttctttat ttactttcta
     9061 aaatccaaat acaaaacata aaaataaata aacacagagt aaattcccaa attattccat
     9121 cattaaaaga tacgaggcgc gtgtaagtta caggcaagcg atccgtcagg tggcactttt
     9181 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     9241 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     9301 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     9361 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     9421 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     9481 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     9541 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     9601 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     9661 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     9721 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     9781 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     9841 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9901 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9961 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    10021 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    10081 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    10141 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    10201 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    10261 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    10321 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    10381 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    10441 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    10501 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    10561 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    10621 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    10681 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    10741 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    10801 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    10861 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    10921 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    10981 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    11041 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    11101 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
    11161 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    11221 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    11281 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
    11341 attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgcggctgc
    11401 aggtcgagtt ta
//
