LOCUS       sequence-178364-       11937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11937)
  AUTHORS   Ledoux S, Guthrie C
  TITLE     Retinitis Pigmentosa Mutations in Bad Response to Refrigeration 2 
            (Brr2) Impair ATPase and Helicase Activity.
  JOURNAL   J Biol Chem. 2016 Jun 3;291(23):11954-65. doi: 
            10.1074/jbc.M115.710848. Epub 2016 Apr 12.
  PUBMED    27072132
REFERENCE   2  (bases 1 to 11937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.710848"; journalName: "J Biol Chem"; date: 
            "2016-06-3- 3"; volume: "291"; issue: "23"; pages: "11954-65"
COMMENT     Sequence Label: pSL6 - Brr2 del247 WT
FEATURES             Location/Qualifiers
     source          1..11937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..644
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     537..560
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             6424..6501
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             6529..6549
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             6589..6762
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             6763..6936
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     primer_bind     complement(7000..7016)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7000..7016)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7042..7061)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7043..7061)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7071..7088)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7071..7087)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7080..7102)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7228..7683
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7315..7334)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7525..7546
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7943..8605)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(8606..8793)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(8940..8959)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9059..9081
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9119..9137)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    9185..9688
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        9718..9822
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9823..9891,9892..10683)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10041..10060)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10854..11442
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11343..11362
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11596..11613
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11730..11751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11766..11771,11772..11789,11790..11796)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11804..11820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11809..11831
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11828..11844
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11828..11844
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11863..11883
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11865..11883
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcattatcaa tactgccatt tcaaagaata cgtaaataat taatagtagt gattttccta
       61 actttattta gtcaaaaaat tagcctttta attctgctgt aacccgtaca tgcccaaaat
      121 agggggcggg ttacacagaa tatataacat cgtaggtgtc tgggtgaaca gtttattcct
      181 ggcatccact aaatataatg gagcccgctt tttaagctgg catccagaaa aaaaaagaat
      241 cccagcacca aaatattgtt ttcttcacca accatcagtt cataggtcca ttctcttagc
      301 gcaactacag agaacagggg cacaaacagg caaaaaacgg gcacaacctc aatggagtga
      361 tgcaacctgc ctggagtaaa tgatgacaca aggcaattga cccacgcatg tatctatctc
      421 attttcttac accttctatt accttctgct ctctctgatt cggaaaaagc tgaaaaaaaa
      481 ggttgaaacc agttccctga aattactccc ctacttgact aataagtata taaagacggt
      541 aggtattgat tgtaattctg taaatctatt tcttaaactt cttaaattct acttttatag
      601 ttagtctttt ttttagtttt aaaacaccaa gaacttagtt tcgactctag aatgtacaat
      661 gacgttgaag tcaattctaa aaaaaaaaac aagagggcat tgccaaacat tgaaaacgat
      721 ataattaaac tctccgatag taaaacaagt aatatcgaga gcgttccgat atactcaatc
      781 gatgaatttt ttttgcagag aaaacttcgg tcagagcttg gttacaagga tacctctgta
      841 atacaagacc tgtcagaaaa aattttgaat gatattgaaa cacttgagca taatccagta
      901 gcgttggagc agaagttggt tgatctgctt aaattcgaga atatatcact ggctgaattt
      961 attctcaaga atagatctac aattttttgg ggcatacgac tggcgaagag cacagagaat
     1021 gaaataccga acttaataga gaaaatggtt gcaaaaggtt tgaatgatct tgtggaacag
     1081 tataaattta gagaaactac acactcaaag cgtgaactcg attccggtga cgaccagcca
     1141 cagtcttcgg aagcaaaaag aacaaaattc agtaatccag caataccgcc cgtcatagat
     1201 ttggaaaaga ttaaattcga cgaaagttct aaattaatga ccgtgacaaa agtatcccta
     1261 ccggaaggat cattcaaaag ggtgaaacca caatatgacg aaatacatat tccagctcct
     1321 agcaaacctg ttattgatta tgagttgaaa gaaattactt ctctaccaga ttggtgtcag
     1381 gaggcgtttc cttcatcgga aacaacgtcc ttaaacccta tacaatcaaa ggtttttcac
     1441 gcagcatttg aaggtgattc aaacatgcta atatgtgcac ccactggttc aggtaagacg
     1501 aatatcgcgt tattgacggt attgaaagcc ttatcccatc attataaccc gaaaactaag
     1561 aaattaaacc tttctgcatt taaaattgtt tatatagcac ccttgaaagc cttagtacag
     1621 gaacaagtcc gagaatttca aaggaggttg gcttttctag gtataaaagt cgctgaattg
     1681 actggtgatt ctaggcttag tagaaagcaa attgacgaga cgcaggtttt ggtgtctacg
     1741 ccagagaaat gggacataac tactagaaat tcaaataatt tggccatcgt agaacttgtt
     1801 cgtttgttga ttatagatga aatacatttg ttacatgatg acaggggacc agtattggaa
     1861 agcatcgtgg ctaggacatt ttgggcttcc aaatatggcc aggaataccc cagaataata
     1921 ggcctttctg ccaccttacc aaattatgag gatgtcggta ggtttttaag agttccgaag
     1981 gaaggattgt tttattttga ctcctcattt agaccgtgtc ctctatctca acagttttgt
     2041 ggcattaagg aacggaactc cttgaaaaag ctgaaggcta tgaatgatgc ttgttatgaa
     2101 aaggtattgg aatccatcaa tgaaggtaat caaataatcg tgtttgttca ttctcgaaaa
     2161 gaaacatcac gaacagcaac atggttgaaa aataaatttg ctgaagagaa cattactcat
     2221 aaattaacca aaaatgatgc aggatcgaag cagattttaa agacagaagc agccaacgta
     2281 ctcgatccaa gtctacgaaa gttgattgaa agcggtatag ggacgcatca cgcagggctg
     2341 accagaagtg acagatcctt atctgaagat ttatttgcag acgggttgtt gcaagtattg
     2401 gtatgcactg cgacgttagc atggggtgtt aatctacctg ctcatacagt gattataaaa
     2461 ggaacagatg tctattcacc tgaaaagggg tcctgggaac aactttctcc acaagatgta
     2521 cttcaaatgt taggtagagc aggcagaccg agatatgaca catttggcga gggtattata
     2581 ataacagacc aatcgaatgt tcaatattat ctctcggttt taaatcaaca attaccaata
     2641 gaatcgcaat ttgtttcaaa attagttgac aacttaaatg cagaagttgt cgcgggtaac
     2701 attaagtgca ggaatgatgc tgtgaattgg ttggcgtata cttacctata tgtcagaatg
     2761 ttagcttccc ctatgcttta caaagtacct gatatttcaa gtgatgggca attgaaaaag
     2821 tttagagaaa gcttagtcca ttcggcccta tgcattttaa aagaacaaga gttggtgtta
     2881 tatgatgctg aaaatgatgt aattgaggcg accgatctag gaaatatagc gtcgtcattt
     2941 tacataaatc atgcttccat ggatgtttac aatagagagt tagatgaaca tacaactcag
     3001 attgatcttt ttagaatatt ctcaatgtcg gaagagttta agtacgtatc ggtgagatat
     3061 gaagagaaaa gagaactaaa acaacttcta gagaaagcac ccattcctat cagagaagat
     3121 attgatgatc ctttagcaaa agtgaatgta ctactgcagt cttatttttc ccagctaaag
     3181 ttcgaaggtt ttgcacttaa ttctgatata gtgtttattc accaaaatgc tggtaggtta
     3241 ttgcgtgcta tgtttgaaat atgcttgaag agaggatggg gacatcccac acggatgttg
     3301 ttaaatcttt gcaaatctgc cacaactaaa atgtggccaa caaactgtcc tttaagacag
     3361 tttaaaacat gccctgtcga agtgattaaa aggctggaag cctctacagt accgtgggga
     3421 gattatctac agctagaaac acctgccgaa gttggtcgtg caatcagatc tgaaaaatat
     3481 ggaaaacagg tttacgattt attgaaaaga tttcccaaaa tgtccgtaac atgcaacgct
     3541 cagccaatta cccgtagcgt tatgaggttc aacattgaaa taatagccga ctggatatgg
     3601 gatatgaatg tacacggttc ccttgaacct tttttgctca tgctagaaga cactgatggt
     3661 gattcaatac tttattatga cgtactattc atcactccag atatagttgg tcatgaattt
     3721 accttatcct ttacttatga gttaaagcag cacaatcaaa ataacttacc acctaatttc
     3781 ttccttactt tgatatcaga aaattggtgg cacagtgaat tcgagattcc agtttccttc
     3841 aatggattta aacttccgaa aaaatttcct cctcctactc cccttctgga gaatattagt
     3901 atttcaacat cagagcttgg caatgatgat ttttccgaag tatttgaatt taaaactttc
     3961 aacaagattc agagccaggt cttcgagtct ctctacaatt cgaatgactc ggtctttgta
     4021 ggttcaggaa agggcacagg taaaacagct atggctgaat tagcactgtt aaatcattgg
     4081 agacagaata aaggaagagc ggtttatatc aatccgtctg gggaaaaaat tgattttttg
     4141 ctatctgatt ggaataaaag gttctctcat cttgctggtg gtaagataat aaataagcta
     4201 ggaaatgatc cttcactaaa tttaaaatta ttagcaaaaa gccatgttct cttggctacc
     4261 cccgtgcaat ttgagctttt gtcacgtcgt tggagacaaa ggaagaacat ccaaagttta
     4321 gaattaatga tatatgatga cgctcatgag attagccaag gtgtttacgg tgctgtgtat
     4381 gaaacgctaa tttctagaat gatatttata gcaacccaac ttgaaaagaa aattcgattt
     4441 gtttgccttt cgaactgttt ggcaaatgct cgtgattttg gtgaatgggc tggtatgacg
     4501 aagtcaaaca tctacaactt ttctccaagc gaaagaattg agcctcttga aattaatatt
     4561 cagtcattta aagatgttga gcacatatca ttcaattttt cgatgcttca aatggcattt
     4621 gaagcatctg ctgctgcagc gggaaatcgt aattccagtt cggtgttctt accatctaga
     4681 aaggattgta tggaagttgc gtctgcattt atgaagtttt caaaagcaat tgaatgggat
     4741 atgttgaacg ttgaagaaga gcaaattgta ccatacattg aaaagttaac agatggtcat
     4801 ctaagagcac ctttaaaaca cggtgttggt attttgtata aggggatggc atcaaatgat
     4861 gaaagaattg tgaaaaggtt atacgaatat ggtgcggtat cggttttact catttctaag
     4921 gattgttctg cattcgcttg taaaactgat gaagtgatta ttttaggaac caacctttat
     4981 gatggtgcag agcataaata tatgccatat accataaatg aacttctaga aatggtggga
     5041 ctagctagtg gtaacgattc aatggcaggc aaggttttaa ttttgacaag tcacaacatg
     5101 aaggcctact acaaaaaatt cttaattgag cctttgccta ctgagagtta cctacagtat
     5161 attattcatg atactctgaa caacgagata gcaaattcta ttatacaaag caaacaagat
     5221 tgtgttgact ggtttacata ttcgtatttc tatcgtcgta tccatgtgaa tccaagctat
     5281 tatggcgtaa gggatacgtc tccacatgga atttcagttt ttttgagtaa tttagttgaa
     5341 acttgtttga atgacttagt tgaatcatcc ttcattgaaa ttgacgatac agaagctgaa
     5401 gtaaccgcag aagttaatgg aggagatgat gaagcaactg aaatcatttc taccttgagc
     5461 aacgggctaa ttgcttctca ctatggcgta tcatttttta ctattcagtc attcgtttct
     5521 tctttatcga atacgtcaac gctcaaaaac atgctgtatg ttctttcaac agcagtagaa
     5581 ttcgaaagtg ttccactcag aaaaggagac agagcattac ttgtcaaatt aagtaaaaga
     5641 ctaccattga gattccctga acacacctct tctggttcgg tgagctttaa agttttctta
     5701 ttgttacaag catatttttc acgtcttgaa ttacctgtcg actttcaaaa tgatttaaaa
     5761 gacattctag aaaaggttgt tccactaatt aacgtagtgg ttgatatcct ttcagcaaac
     5821 gggtatttga atgctactac cgccatggat ttggcgcaga tgttgattca gggtgtatgg
     5881 gacgttgata atccgttgag acagattcca cacttcaaca acaaaatttt ggaaaaatgt
     5941 aaagaaatta acgtcgagac ggtctatgat ataatggccc ttgaggatga agagagggat
     6001 gaaattttaa cgcttactga ttcacagctt gcgcaggtcg ccgcgtttgt taacaattac
     6061 cccaacgttg aacttactta ttcgttgaat aattctgatt cgttaatttc aggtgtaaaa
     6121 cagaaaatta ccattcaatt gacaagagat gttgaacctg agaacttaca agtaacatct
     6181 gaaaagtatc cgtttgataa gctagaaagt tggtggttgg ttttaggtga agtatctaaa
     6241 aaagagcttt atgcgatcaa gaaagttact ttaaataaag agactcagca atatgaattg
     6301 gaatttgaca ctccgacatc tggtaaacat aacttaacaa tttggtgtgt ctgtgattca
     6361 tatcttgacg cagataaaga gttgtccttt gaaataaatg tgaaactcga gggatccatg
     6421 gaaaagagaa gatggaaaaa gaatttcata gccgtctcag cagccaaccg ctttaagaaa
     6481 atctcatcct ccggggcact tgattatgat attccaacta ctgctagcga gaatttgtat
     6541 tttcagggtg agctcaaaac cgcggctctt gcgcaacacg atgaagccgt ggacaacaaa
     6601 ttcaacaaag aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa
     6661 gaacaacgaa acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt
     6721 ttagcagaag ctaaaaagct aaatgatgct caggcgccga aagtagacaa caaattcaac
     6781 aaagagcaac aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa cgaagaacaa
     6841 cgaaacgcct tcatccaaag tttaaaagat gacccaagcc aaagcgctaa ccttttagca
     6901 gaagctaaaa agctaaatgg tgctcaggcg ccgaaagtag acgcgaattc cgcggggaag
     6961 tcaacctgaa gcttgatatc gaattcctgc agcccggggg atccactagt tctagagcgg
     7021 ccgggcccgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     7081 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7141 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7201 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     7261 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     7321 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7381 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7441 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     7501 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7561 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7621 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     7681 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagatcc
     7741 gtcgagttca agagaaaaaa aaagaaaaag caaaaagaaa aaaggaaagc gcgcctcgtt
     7801 cagaatgaca cgtatagaat gatgcattac cttgtcatct tcagtatcat actgttcgta
     7861 tacatactta ctgacattca taggtataca tatatacaca tgtatatata tcgtatgctg
     7921 cagctttaaa taatcggtgt cactacataa gaacaccttt ggtggaggga acatcgttgg
     7981 taccattggg cgaggtggct tctcttatgg caaccgcaag agccttgaac gcactctcac
     8041 tacggtgatg atcattcttg cctcgcagac aatcaacgtg gagggtaatt ctgctagcct
     8101 ctgcaaagct ttcaagaaaa tgcgggatca tctcgcaaga gagatctcct actttctccc
     8161 tttgcaaacc aagttcgaca actgcgtacg gcctgttcga aagatctacc accgctctgg
     8221 aaagtgcctc atccaaaggc gcaaatcctg atccaaacct ttttactcca cgcacggccc
     8281 ctagggcctc tttaaaagct tgaccgagag caatcccgca gtcttcagtg gtgtgatggt
     8341 cgtctatgtg taagtcacca atgcactcaa cgattagcga ccagccggaa tgcttggcca
     8401 gagcatgtat catatggtcc agaaacccta tacctgtgtg gacgttaatc acttgcgatt
     8461 gtgtggcctg ttctgctact gcttctgcct ctttttctgg gaagatcgag tgctctatcg
     8521 ctaggggacc accctttaaa gagatcgcaa tctgaatctt ggtttcattt gtaatacgct
     8581 ttactagggc tttctgctct gtcatctttg ccttcgttta tcttgcctgc tcatttttta
     8641 gtatattctt cgaagaaatc acattacttt atataatgta taattcatta tgtgataatg
     8701 ccaatcgcta agaaaaaaaa agagtcatcc gctaggtgga aaaaaaaaaa tgaaaatcat
     8761 taccgaggca taaaaaaata tagagtgtac tagaggaggc caagagtaat agaaaaagaa
     8821 aattgcggga aaggactgtg ttatgacttc cctgactaat gccgtgttca aacgatacct
     8881 ggcagtgact cctagcgctc accaagctct taaaacggga atttatggtg cactctcagt
     8941 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     9001 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     9061 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     9121 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagggtcct
     9181 tttcatcacg tgctataaaa ataattataa tttaaatttt ttaatataaa tatataaatt
     9241 aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc cgaagatgta
     9301 aaagactcta gggggatcgc caacaaatac taccttttat cttgctcttc ctgctctcag
     9361 gtattaatgc cgaattgttt catcttgtct gtgtagaaga ccacacacga aaatcctgtg
     9421 attttacatt ttacttatcg ttaatcgaat gtatatctat ttaatctgct tttcttgtct
     9481 aataaatata tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt gtttattttt
     9541 ttttcttcat tccgtaactc ttctaccttc tttatttact ttctaaaatc caaatacaaa
     9601 acataaaaat aaataaacac agagtaaatt cccaaattat tccatcatta aaagatacga
     9661 ggcgcgtgta agttacaggc aagcgatccg tcaggtggca cttttcgggg aaatgtgcgc
     9721 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     9781 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     9841 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     9901 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     9961 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
    10021 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
    10081 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
    10141 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
    10201 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
    10261 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
    10321 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
    10381 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
    10441 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
    10501 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
    10561 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    10621 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    10681 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    10741 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    10801 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    10861 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    10921 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    10981 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    11041 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    11101 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    11161 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    11221 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    11281 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    11341 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    11401 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    11461 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    11521 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    11581 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
    11641 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
    11701 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
    11761 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
    11821 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
    11881 agggaacaaa agctggagct ccaccgcggt ggcggccgcg gctgcaggtc gagttta
//
