LOCUS       sequence-178369-        4848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pmec-4::mcherry, Expresses mcherry red fluorescence in C. elegans 
            touch neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Xu S, Chisholm AD
  TITLE     Highly efficient optogenetic cell ablation in C. elegans using 
            membrane-targeted miniSOG.
  JOURNAL   Sci Rep. 2016 Feb 10;6:21271. doi: 10.1038/srep21271.
  PUBMED    26861262
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-10"; volume: "6:21271. doi"; pages: " 
            10.1038/srep21271"
COMMENT     Sequence Label: pCZGY546
FEATURES             Location/Qualifiers
     source          1..4848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     24..41
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    158..179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(194..199,200..217,218..224)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    232..248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     237..259
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     256..272
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     256..272
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    336..360
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1416..1440)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2685..2707)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2807..2826
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2908..2926)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2994..3098
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3099..3167,3168..3959)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3317..3336)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4130..4718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4619..4638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
       61 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      121 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      181 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      241 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt gcatgcacta
      301 gactcgaggt agacggtatc gataagcttg atatcacaag tttgtacaaa aaagcaggct
      361 ccgaattcgc ccttaagctt caatacaagc tcaaatacaa tactattttt tttccgtctg
      421 aacataaaat tagtttctaa ataaaaaatg tgaaaataaa tatttggttt taatcgttaa
      481 aatcttccaa attactaata aagttcagtc aactatatgc ttcgaaattc actgaatcta
      541 ttattgttat taatttttag acccttggag agctcccaac tctttcaatc cagaagactg
      601 atggatgcca ggtgtacttg tcaaaggtgg ctcaaggatg cgagattgtc acttccaaat
      661 ccagtgagat gaacatctcc gtgcaaacca atgatgatgg ggattatgta agtttttcgg
      721 attgtagaac ccaatctaaa tttccttttc agtcggagtt cccggttccg gagcagttca
      781 agacaacttt tgtcaatggc aagctagtca ctgtcgtctc ggacatttgc taaatcttct
      841 cacgtcataa ccttattttt caacttcatg aactatcccc tcgaactttt ttatttgtcc
      901 ctgtctacaa ttctttataa gcaattgaga cacactttgc tttattaaat aataccgatt
      961 ttgttttgta tatgcattga ctggtgctca actattttta ttgaaaaaat tactttttac
     1021 tgtctcgtcg aataaaagtg atcccttcca aaaaatagtt attcatgttt gaaggtttgt
     1081 gatcgtagaa caattgcacc gaatgtagtg taaaaaaaat tccagaatgt tcaaatacat
     1141 ttccaccctc accagaacat ctcaaacacc aggcacattt caacacactt tcatggatct
     1201 tatcttgcct caaaaccatc agctttcttc tcattctaca agtatgaaac aatctactat
     1261 tctatacatt tataatgtaa tggaaccatg tgaagacatg agcaagatca ttgtcgtcag
     1321 cgactacccc tccttcattt ttcttagagt atcagtttgc taatgctatt ttttttatcg
     1381 ctatcaagtt atagagggat ccaagggcga attcgaccca gctttcttgt acaaagtggt
     1441 gatatcgaat tcctgcagcc cgggggatcc actagtctcc atggtctcaa agggtgaaga
     1501 agataacatg gcaattatta aagagtttat gcgtttcaag gtgcatatgg agggatctgt
     1561 caatgggcat gagtttgaaa ttgaaggtga aggagaaggc cgaccatatg agggaacaca
     1621 aaccgcaaaa ctaaaggtaa gtttaaacat atatatacta actaaccctg attatttaaa
     1681 ttttcaggta actaaaggcg gaccattacc attcgcctgg gacatcctct ctccacagtt
     1741 catgtatgga agtaaagctt atgttaaaca tccggcagat ataccagatt atttgaaact
     1801 ttcattcccg gagggtttta agtgggaacg cgtaatgaat tttgaagacg gaggagttgt
     1861 tacagtgacg caagactcaa ggtaagttta aacagttcgg tactaactaa ccatacatat
     1921 ttaaattttc agcctccaag atggagaatt tatttataaa gtcaaacttc gaggaacgaa
     1981 tttcccctcg gatggacctg ttatgcagaa gaagactatg ggatgggaag cttcaagtga
     2041 aagaatgtac cctgaagacg gtgctcttaa gggagagatt aaacaacgtc ttaaattgaa
     2101 agatggagga cattacgatg ctgaggtaag tttaaacatg attttactaa ctaactaatc
     2161 tgatttaaat tttcaggtga agacaactta caaagccaaa aaaccagttc agctgccagg
     2221 agcgtacaat gttaatatta aactggatat cacctcccac aacgaggatt acactatcgt
     2281 tgagcaatat gaaagagctg aagggcggca ctcgacaggt ggcatggatg aattgtataa
     2341 ggctagcggt ggagcataat gaccgcggat aacaaatttc atatgttttt gtttgttttt
     2401 tgtcttaatt accacaggtc atttctctct ttctatcgtc attttcttgt cattttttct
     2461 ttcttgagca ttggcaacaa atttattcgt tatgcattat gggtacttgt ttctcgcttt
     2521 ttaatgtata atctcaattc ttgaactaaa actctcagtg cttctgaaaa ttatggttac
     2581 aatgaattag tttattaaat ttattatttg aattaatacc gaaacattta aatattaacg
     2641 ccagggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2701 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     2761 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     2821 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     2881 taccgcatca ggcggcctta attaagggcc tcgtgatacg cctattttta taggttaatg
     2941 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3001 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3061 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3121 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     3181 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3241 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     3301 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     3361 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3421 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3481 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3541 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3601 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     3661 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3721 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     3781 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     3841 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3901 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3961 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4021 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4081 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4141 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4201 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4261 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4321 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4381 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4441 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4501 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4561 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4621 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4681 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4741 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4801 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctc
//
