LOCUS       sequence-178386-        4315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dimeric LacI (truncated last 11 amino acids) polymorphism with T at 
            position 109.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Meinhardt S, Manley MW Jr, Becker NA, Hessman JA, Maher LJ 3rd, 
            Swint-Kruse L
  TITLE     Novel insights from hybrid LacI/GalR proteins: family-wide 
            functional attributes and biologically significant variation in 
            transcription repression.
  JOURNAL   Nucleic Acids Res. 2012 Nov;40(21):11139-54. doi: 
            10.1093/nar/gks806. Epub 2012 Sep 10.
  PUBMED    22965134
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gks806"; journalName: "Nucleic Acids Res"; date: 
            "2012-11"; volume: "40"; issue: "21"; pages: "11139-54"
COMMENT     Sequence Label: LacI-11/109-pHG165c
FEATURES             Location/Qualifiers
     source          1..4315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(106..125)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             1673..1864
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1858..1880)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1966..2106
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2021..2040
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2121..2138)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2292..2880)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2372..2391)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3051..3842,3843..3911))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3674..3693
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3912..4016)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4084..4102
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     4255..4277
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4269..4286
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4270..4286
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccgggaattc caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg
       61 aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt
      121 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
      181 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
      241 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
      301 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg
      361 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa acggcggtgc
      421 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg
      481 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
      541 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
      601 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg
      661 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc
      721 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa
      781 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg
      841 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
      901 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg
      961 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
     1021 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc
     1081 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtgatgag
     1141 aattcgtaat aatatagctc tagattaatt gcgcccgtta acggcgggca gcgttgggtc
     1201 ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
     1261 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
     1321 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     1381 aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
     1441 atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
     1501 ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
     1561 ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
     1621 catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa
     1681 acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat taacgcttct
     1741 ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga
     1801 ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     1861 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     1921 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     1981 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     2041 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2101 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2161 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2221 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2281 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2341 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2401 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2461 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2521 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2581 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2641 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2701 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     2761 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2821 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2881 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2941 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3001 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3061 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3121 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3181 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3241 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3301 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3361 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3421 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3481 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3541 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3601 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3661 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3721 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3781 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3841 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3901 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3961 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4021 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     4081 aaaaataggc gtatcacgag gccctttcgt cttcaagaat ccgttaacgg cattcgccat
     4141 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     4201 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     4261 cacgacgttg taaaacgacg gccagtgcca agcttggctg caggtcgacg gatcc
//
