LOCUS       sequence-178401-        4671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full length WT FruR (CRA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4671)
  TITLE     Swint-Kruse lab plasmids
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FruR-pHG165a (CRA)
FEATURES             Location/Qualifiers
     source          1..4671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..88
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(1340..1357)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1340..1356)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1349..1371)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1524..1542)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1610..1714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1715..1783,1784..2575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1933..1952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2746..3334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3235..3254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3488..3505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3520..3660
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3586..3605)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3746..3768
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3762..3953)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     protein_bind    4553..4574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4589..4594,4595..4612,4613..4619)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     4649..4665
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4649..4665
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacgaattcc gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc
       61 ggaagagagt caattcaggg tggtgaatgt gaaactggat gaaatcgctc ggctggcggg
      121 agtgtcgcgg accactgcaa gctatgttat taacggcaaa gcgaagcaat accgtgtgag
      181 cgacaaaacc gttgaaaaag tcatggctgt ggtgcgtgag cacaattacc acccgaacgc
      241 cgtggcagct gggcttcgtg ctggacgcac acgttctatt ggtcttgtga tccccgatct
      301 ggagaacacc agctataccc gcatcgctaa ctatcttgaa cgccaggcgc ggcaacgggg
      361 ttatcaactg ctgattgcct gctcagaaga tcagccagac aacgaaatgc ggtgcattga
      421 gcacctttta cagcgtcagg ttgatgccat tattgtttcg acgtcgttgc ctcctgagca
      481 tcctttttat caacgctggg ctaacgaccc gttcccgatt gtcgcgctgg accgcgccct
      541 cgatcgtgaa cacttcacca gcgtggttgg tgccgatcag gatgatgccg aaatgctggc
      601 ggaagagtta cgtaagtttc ccgccgagac ggtgctttat cttggtgcgc taccggagct
      661 ttctgtcagc ttcctgcgtg aacaaggttt ccgtactgcc tggaaagatg atccgcgcga
      721 agtgcatttc ctgtatgcca acagctatga gcgggaggcg gctgcccagt tattcgaaaa
      781 atggctggaa acgcatccga tgccgcaggc gctgttcaca acgtcgtttg cgttgttgca
      841 aggagtgatg gatgtcacgc tgcgtcgcga cggcaaactg ccttctgacc tggcaattgc
      901 cacctttggc gataacgaac tgctcgactt cttacagtgt ccggtgctgg cagtggctca
      961 acgtcaccgc gatgtcgcag agcgtgtgct ggagattgtc ctggcaagcc tggacgaacc
     1021 gcgtaagcca aaacctggtt taacgcgcat taaacgtaat ctctatcgcc gcggcgtgct
     1081 cagccgtagc taataacctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca
     1141 cggttacgat gcgcccatct acaccaacgt gacctatccc attaggtcaa tccgccgttt
     1201 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg
     1261 ctacaggaag gccagacgcg aattattttt gatggcgttg gaattcccgg ggatccgtcg
     1321 acctgcagcc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     1381 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     1441 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgccgtt
     1501 aacggattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     1561 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1621 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1681 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1741 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1801 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1861 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1921 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     1981 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     2041 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     2101 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2161 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2221 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     2281 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2341 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2401 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     2461 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2521 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactatc
     2581 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2641 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2701 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2761 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2821 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2881 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2941 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3001 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3061 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3121 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3181 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3241 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3301 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3361 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3421 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3481 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     3541 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     3601 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     3661 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     3721 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     3781 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca
     3841 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
     3901 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
     3961 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
     4021 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
     4081 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
     4141 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
     4201 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
     4261 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
     4321 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
     4381 cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc
     4441 gccgttaacg gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4501 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     4561 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     4621 gtggcgtggt actcataaca atttcacaca ggaaacagct atgaccatga t
//
