LOCUS       sequence-178405-       10812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To convert human skin fibroblasts into induced motor neurons (hiMN) 
            in combination with NGN2, Sox11, FGF2 and two small molecules, 
            forskolin and dorsomorphin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Liu ML, Zang T, Zhang CL
  TITLE     Direct Lineage Reprogramming Reveals Disease-Specific Phenotypes of 
            Motor Neurons from Human ALS Patients.
  JOURNAL   Cell Rep. 2016 Jan 5;14(1):115-28. doi: 
            10.1016/j.celrep.2015.12.018. Epub 2015 Dec 24.
  PUBMED    26725112
REFERENCE   2  (bases 1 to 10812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.12.018"; journalName: "Cell Rep"; date: 
            "2016-01-5- 5"; volume: "14"; issue: "1"; pages: "115-28"
COMMENT     Sequence Label: pCSC-ISL1-T2A-LHX3
FEATURES             Location/Qualifiers
     source          1..10812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..583
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             601..781
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    828..953
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1446..1679
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1864..1908
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2160..2276
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2299..2602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2603..2806
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2803..2827
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2895..2904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3691..3700
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3975..4028
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4035..4044
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5388..5976
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5441..5461)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5859..5870)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6383..6563
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6589..6606)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6595..6819
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6865..7293
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6952..6971)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7162..7183
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7302..7322)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7307..7636
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7487..7622
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7684..7731
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7750..8124
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8254..8375
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8291..8310)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8345..8364
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8424..8440)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8424..8440)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8437..8459)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8448..8464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8472..8478,8479..8496,8497..8502))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8517..8538
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8655..8672)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8826..9414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8906..8925)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9585..10376,10377..10445))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10208..10227
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10446..10550)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10625..10644)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
      601 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
      661 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      721 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      781 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      841 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      901 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      961 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     1021 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     1081 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     1141 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     1201 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     1261 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     1321 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1381 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1441 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1501 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1561 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1621 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1681 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1741 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1801 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1861 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1921 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1981 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2041 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2101 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcaact
     2161 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     2221 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     2281 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2341 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2401 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2461 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2521 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2581 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2641 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2701 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2761 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     2821 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
     2881 gaggatccac cggtgccacc atgggagaca tgggagatcc accaaaaaaa aaacgtctga
     2941 tttccctatg tgttggttgc ggcaatcaga ttcacgatca gtatattctg agggtttctc
     3001 cggatttgga atggcatgcg gcatgtttga aatgtgcgga gtgtaatcag tatttggacg
     3061 agagctgtac atgctttgtt agggatggga aaacctactg taaaagagat tatatcaggt
     3121 tgtacgggat caaatgcgcc aagtgcagca tcggcttcag caagaacgac ttcgtgatgc
     3181 gtgcccgctc caaggtgtat cacatcgagt gtttccgctg tgtggcctgc agccgccagc
     3241 tcatccctgg ggacgaattt gcgcttcggg aggacggtct cttctgccga gcagaccacg
     3301 atgtggtgga gagggccagt ctaggcgctg gcgacccgct cagtcccctg catccagcgc
     3361 ggccactgca aatggcagcg gagcccatct ccgccaggca gccggccctg cggccccacg
     3421 tccacaagca gccggagaag accacccgcg tgcggactgt gctgaacgag aagcagctgc
     3481 acaccttgcg gacctgctac gccgcaaacc cgcggccaga tgcgctcatg aaggagcaac
     3541 tggtagagat gacgggcctc agtccccgtg tgatccgggt ctggtttcaa aacaagcggt
     3601 gcaaggacaa gaagcgaagc atcatgatga agcaactcca gcagcagcag cccaatgaca
     3661 aaactaatat ccaggggatg acaggaactc ccatggtggc tgccagtcca gagagacacg
     3721 acggtggctt acaggctaac ccagtggaag tacaaagtta ccagccacct tggaaagtac
     3781 tgagcgactt cgccttgcag agtgacatag atcagcctgc ttttcagcaa ctggtcaatt
     3841 tttcagaagg aggaccgggc tctaattcca ctggcagtga agtagcatca atgtcctctc
     3901 aacttccaga tacacctaac agcatggtag ccagtcctat tgaggcaagg tacaagtccg
     3961 gactcagatc ctacgagggg agaggaagtc ttctgacctg cggagacgtc gaagagaatc
     4021 ctggacccgc tagagccacc atggaggcgc gcggggagct gggcccggcc cgggagtcgg
     4081 cgggaggcga cctgctgcta gcactgctgg cgcggagggc ggacctgcgc cgagagatcc
     4141 cgctgtgcgc tggctgtgac cagcacatcc tggaccgctt catcctcaag gctctggacc
     4201 gccactggca cagcaagtgt ctcaagtgca gcgactgcca cacgccactg gccgagcgct
     4261 gcttcagccg aggggagagc gtttactgca aggacgactt tttcaagcgc ttcgggacca
     4321 agtgcgccgc gtgccagctg ggcatcccgc ccacgcaggt ggtgcgccgc gcccaggact
     4381 tcgtgtacca cctgcactgc tttgcctgcg tcgtgtgcaa gcggcagctg gccacgggcg
     4441 acgagttcta cctcatggag gacagccggc tcgtgtgcaa ggcggactac gaaaccgcca
     4501 agcagcgaga ggccgaggcc acggccaagc ggccgcgcac gaccatcacc gccaagcagc
     4561 tggagacgct gaagagcgct tacaacacct cgcccaagcc ggcgcgccac gtgcgcgagc
     4621 agctctcgtc cgagacgggc ctggacatgc gcgtggtgca ggtttggttc cagaaccgcc
     4681 gggccaagga gaagaggctg aagaaggacg ccggccggca gcgctggggg cagtatttcc
     4741 gcaacatgaa gcgctcccgc ggcggctcca agtcggacaa ggacagcgtt caggaggggc
     4801 aggacagcga cgctgaggtc tccttccccg atgagccttc cttggcggaa atgggcccgg
     4861 ccaatggcct ctacgggagc ttgggggaac ccacccaggc cttgggccgg ccctcgggag
     4921 ccctgggcaa cttctccctg gagcatggag gcctggcagg cccagagcag taccgagagc
     4981 tgcgtcccgg cagcccctac ggtgtccccc catcccccgc cgccccgcag agcctccctg
     5041 gcccccagcc cctcctctcc agcctggtgt acccagacac cagcttgggc cttgtgccct
     5101 cgggagcccc cggcgggccc ccacccatga gggtgctggc agggaacgga cccagttctg
     5161 acctatccac ggggagcagc gggggttacc ccgacttccc tgccagcccc gcctcctggc
     5221 tggatgaggt agaccacgct cagttctaac tcgagggatc aagcttatcg aaacctggaa
     5281 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     5341 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcagacaat caacctctgg
     5401 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5461 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5521 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5581 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5641 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5701 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5761 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     5821 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5881 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5941 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt
     6001 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6061 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     6121 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     6181 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     6241 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     6301 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     6361 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6421 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6481 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6541 gtcagtgtgg aaaatctcta gcagggccgc tttaaacccg ctgatcagcc tcgactgtgc
     6601 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     6661 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     6721 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     6781 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     6841 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6901 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6961 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7021 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7081 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7141 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7201 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7261 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     7321 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7381 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7441 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     7501 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7561 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7621 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     7681 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     7741 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     7801 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     7861 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     7921 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     7981 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     8041 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     8101 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     8161 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     8221 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     8281 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8341 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     8401 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8461 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8521 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8581 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8641 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8701 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8761 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8821 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8881 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8941 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9001 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9061 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9121 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9181 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9241 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9301 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9361 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9421 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9481 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9541 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9601 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9661 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9721 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9781 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9841 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9901 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9961 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10021 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10081 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10141 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10201 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10261 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10321 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10381 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10441 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10501 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10561 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
    10621 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
    10681 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
    10741 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
    10801 ccagatatac gc
//
