LOCUS       sequence-178417-       12336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12336)
  AUTHORS   Ledoux S, Guthrie C
  TITLE     Retinitis Pigmentosa Mutations in Bad Response to Refrigeration 2 
            (Brr2) Impair ATPase and Helicase Activity.
  JOURNAL   J Biol Chem. 2016 Jun 3;291(23):11954-65. doi: 
            10.1074/jbc.M115.710848. Epub 2016 Apr 12.
  PUBMED    27072132
REFERENCE   2  (bases 1 to 12336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.710848"; journalName: "J Biol Chem"; date: 
            "2016-06-3- 3"; volume: "291"; issue: "23"; pages: "11954-65"
COMMENT     Sequence Label: pSL3 - Brr2 del113 N1104L
FEATURES             Location/Qualifiers
     source          1..12336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..644
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     537..560
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             6823..6900
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             6928..6948
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             6988..7161
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             7162..7335
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     primer_bind     complement(7399..7415)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7399..7415)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7441..7460)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7442..7460)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7470..7487)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7470..7486)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7479..7501)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7627..8082
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7714..7733)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7924..7945
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8342..9004)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(9005..9192)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(9339..9358)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9458..9480
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9518..9536)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    9584..10087
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        10117..10221
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10222..10290,10291..11082)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10440..10459)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11253..11841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11742..11761
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11995..12012
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12129..12150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12165..12170,12171..12188,12189..12195)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12203..12219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12208..12230
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12227..12243
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12227..12243
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12262..12282
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12264..12282
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcattatcaa tactgccatt tcaaagaata cgtaaataat taatagtagt gattttccta
       61 actttattta gtcaaaaaat tagcctttta attctgctgt aacccgtaca tgcccaaaat
      121 agggggcggg ttacacagaa tatataacat cgtaggtgtc tgggtgaaca gtttattcct
      181 ggcatccact aaatataatg gagcccgctt tttaagctgg catccagaaa aaaaaagaat
      241 cccagcacca aaatattgtt ttcttcacca accatcagtt cataggtcca ttctcttagc
      301 gcaactacag agaacagggg cacaaacagg caaaaaacgg gcacaacctc aatggagtga
      361 tgcaacctgc ctggagtaaa tgatgacaca aggcaattga cccacgcatg tatctatctc
      421 attttcttac accttctatt accttctgct ctctctgatt cggaaaaagc tgaaaaaaaa
      481 ggttgaaacc agttccctga aattactccc ctacttgact aataagtata taaagacggt
      541 aggtattgat tgtaattctg taaatctatt tcttaaactt cttaaattct acttttatag
      601 ttagtctttt ttttagtttt aaaacaccaa gaacttagtt tcgactctag aatgccctca
      661 aatgtcgaaa cttacgagca gattttgcag tgggttacag aagttttagg caatgatata
      721 ccccatgatc ttattattgg aactgctgat atattcatca gacaattgaa agaaaatgag
      781 gaaaatgagg atggtaatat tgaagagcga aaggaaaaga tccagcatga gctaggcatc
      841 aatatagact ccctgaagtt taacgaatta gttaaactta tgaaaaacat taccgattat
      901 gaaacacatc ctgacaatag caataaacaa gcagtagcaa ttcttgccga tgatgaaaaa
      961 tcagatgaag aagaagtaac agaaatgtcc aacaatgcta atgttcttgg gggagaaatc
     1021 aatgataatg aagatgatga tgaggaatat gattacaatg acgttgaagt caattctaaa
     1081 aaaaaaaaca agagggcatt gccaaacatt gaaaacgata taattaaact ctccgatagt
     1141 aaaacaagta atatcgagag cgttccgata tactcaatcg atgaattttt tttgcagaga
     1201 aaacttcggt cagagcttgg ttacaaggat acctctgtaa tacaagacct gtcagaaaaa
     1261 attttgaatg atattgaaac acttgagcat aatccagtag cgttggagca gaagttggtt
     1321 gatctgctta aattcgagaa tatatcactg gctgaattta ttctcaagaa tagatctaca
     1381 attttttggg gcatacgact ggcgaagagc acagagaatg aaataccgaa cttaatagag
     1441 aaaatggttg caaaaggttt gaatgatctt gtggaacagt ataaatttag agaaactaca
     1501 cactcaaagc gtgaactcga ttccggtgac gaccagccac agtcttcgga agcaaaaaga
     1561 acaaaattca gtaatccagc aataccgccc gtcatagatt tggaaaagat taaattcgac
     1621 gaaagttcta aattaatgac cgtgacaaaa gtatccctac cggaaggatc attcaaaagg
     1681 gtgaaaccac aatatgacga aatacatatt ccagctccta gcaaacctgt tattgattat
     1741 gagttgaaag aaattacttc tctaccagat tggtgtcagg aggcgtttcc ttcatcggaa
     1801 acaacgtcct taaaccctat acaatcaaag gtttttcacg cagcatttga aggtgattca
     1861 aacatgctaa tatgtgcacc cactggttca ggtaagacga atatcgcgtt attgacggta
     1921 ttgaaagcct tatcccatca ttataacccg aaaactaaga aattaaacct ttctgcattt
     1981 aaaattgttt atatagcacc cttgaaagcc ttagtacagg aacaagtccg agaatttcaa
     2041 aggaggttgg cttttctagg tataaaagtc gctgaattga ctggtgattc taggcttagt
     2101 agaaagcaaa ttgacgagac gcaggttttg gtgtctacgc cagagaaatg ggacataact
     2161 actagaaatt caaataattt ggccatcgta gaacttgttc gtttgttgat tatagatgaa
     2221 atacatttgt tacatgatga caggggacca gtattggaaa gcatcgtggc taggacattt
     2281 tgggcttcca aatatggcca ggaatacccc agaataatag gcctttctgc caccttacca
     2341 aattatgagg atgtcggtag gtttttaaga gttccgaagg aaggattgtt ttattttgac
     2401 tcctcattta gaccgtgtcc tctatctcaa cagttttgtg gcattaagga acggaactcc
     2461 ttgaaaaagc tgaaggctat gaatgatgct tgttatgaaa aggtattgga atccatcaat
     2521 gaaggtaatc aaataatcgt gtttgttcat tctcgaaaag aaacatcacg aacagcaaca
     2581 tggttgaaaa ataaatttgc tgaagagaac attactcata aattaaccaa aaatgatgca
     2641 ggatcgaagc agattttaaa gacagaagca gccaacgtac tcgatccaag tctacgaaag
     2701 ttgattgaaa gcggtatagg gacgcatcac gcagggctga ccagaagtga cagatcctta
     2761 tctgaagatt tatttgcaga cgggttgttg caagtattgg tatgcactgc gacgttagca
     2821 tggggtgtta atctacctgc tcatacagtg attataaaag gaacagatgt ctattcacct
     2881 gaaaaggggt cctgggaaca actttctcca caagatgtac ttcaaatgtt aggtagagca
     2941 ggcagaccga gatatgacac atttggcgag ggtattataa taacagacca atcgaatgtt
     3001 caatattatc tctcggtttt aaatcaacaa ttaccaatag aatcgcaatt tgtttcaaaa
     3061 ttagttgaca acttaaatgc agaagttgtc gcgggtaaca ttaagtgcag gaatgatgct
     3121 gtgaattggt tggcgtatac ttacctatat gtcagaatgt tagcttcccc tatgctttac
     3181 aaagtacctg atatttcaag tgatgggcaa ttgaaaaagt ttagagaaag cttagtccat
     3241 tcggccctat gcattttaaa agaacaagag ttggtgttat atgatgctga aaatgatgta
     3301 attgaggcga ccgatctagg aaatatagcg tcgtcatttt acataaatca tgcttccatg
     3361 gatgtttaca atagagagtt agatgaacat acaactcaga ttgatctttt tagaatattc
     3421 tcaatgtcgg aagagtttaa gtacgtatcg gtgagatatg aagagaaaag agaactaaaa
     3481 caacttctag agaaagcacc cattcctatc agagaagata ttgatgatcc tttagcaaaa
     3541 gtgaatgtac tactgcagtc ttatttttcc cagctaaagt tcgaaggttt tgcacttaat
     3601 tctgatatag tgtttattca ccaattggct ggtaggttat tgcgtgctat gtttgaaata
     3661 tgcttgaaga gaggatgggg acatcccaca cggatgttgt taaatctttg caaatctgcc
     3721 acaactaaaa tgtggccaac aaactgtcct ttaagacagt ttaaaacatg ccctgtcgaa
     3781 gtgattaaaa ggctggaagc ctctacagta ccgtggggag attatctaca gctagaaaca
     3841 cctgccgaag ttggtcgtgc aatcagatct gaaaaatatg gaaaacaggt ttacgattta
     3901 ttgaaaagat ttcccaaaat gtccgtaaca tgcaacgctc agccaattac ccgtagcgtt
     3961 atgaggttca acattgaaat aatagccgac tggatatggg atatgaatgt acacggttcc
     4021 cttgaacctt ttttgctcat gctagaagac actgatggtg attcaatact ttattatgac
     4081 gtactattca tcactccaga tatagttggt catgaattta ccttatcctt tacttatgag
     4141 ttaaagcagc acaatcaaaa taacttacca cctaatttct tccttacttt gatatcagaa
     4201 aattggtggc acagtgaatt cgagattcca gtttccttca atggatttaa acttccgaaa
     4261 aaatttcctc ctcctactcc ccttctggag aatattagta tttcaacatc agagcttggc
     4321 aatgatgatt tttccgaagt atttgaattt aaaactttca acaagattca gagccaggtc
     4381 ttcgagtctc tctacaattc gaatgactcg gtctttgtag gttcaggaaa gggcacaggt
     4441 aaaacagcta tggctgaatt agcactgtta aatcattgga gacagaataa aggaagagcg
     4501 gtttatatca atccgtctgg ggaaaaaatt gattttttgc tatctgattg gaataaaagg
     4561 ttctctcatc ttgctggtgg taagataata aataagctag gaaatgatcc ttcactaaat
     4621 ttaaaattat tagcaaaaag ccatgttctc ttggctaccc ccgtgcaatt tgagcttttg
     4681 tcacgtcgtt ggagacaaag gaagaacatc caaagtttag aattaatgat atatgatgac
     4741 gctcatgaga ttagccaagg tgtttacggt gctgtgtatg aaacgctaat ttctagaatg
     4801 atatttatag caacccaact tgaaaagaaa attcgatttg tttgcctttc gaactgtttg
     4861 gcaaatgctc gtgattttgg tgaatgggct ggtatgacga agtcaaacat ctacaacttt
     4921 tctccaagcg aaagaattga gcctcttgaa attaatattc agtcatttaa agatgttgag
     4981 cacatatcat tcaatttttc gatgcttcaa atggcatttg aagcatctgc tgctgcagcg
     5041 ggaaatcgta attccagttc ggtgttctta ccatctagaa aggattgtat ggaagttgcg
     5101 tctgcattta tgaagttttc aaaagcaatt gaatgggata tgttgaacgt tgaagaagag
     5161 caaattgtac catacattga aaagttaaca gatggtcatc taagagcacc tttaaaacac
     5221 ggtgttggta ttttgtataa ggggatggca tcaaatgatg aaagaattgt gaaaaggtta
     5281 tacgaatatg gtgcggtatc ggttttactc atttctaagg attgttctgc attcgcttgt
     5341 aaaactgatg aagtgattat tttaggaacc aacctttatg atggtgcaga gcataaatat
     5401 atgccatata ccataaatga acttctagaa atggtgggac tagctagtgg taacgattca
     5461 atggcaggca aggttttaat tttgacaagt cacaacatga aggcctacta caaaaaattc
     5521 ttaattgagc ctttgcctac tgagagttac ctacagtata ttattcatga tactctgaac
     5581 aacgagatag caaattctat tatacaaagc aaacaagatt gtgttgactg gtttacatat
     5641 tcgtatttct atcgtcgtat ccatgtgaat ccaagctatt atggcgtaag ggatacgtct
     5701 ccacatggaa tttcagtttt tttgagtaat ttagttgaaa cttgtttgaa tgacttagtt
     5761 gaatcatcct tcattgaaat tgacgataca gaagctgaag taaccgcaga agttaatgga
     5821 ggagatgatg aagcaactga aatcatttct accttgagca acgggctaat tgcttctcac
     5881 tatggcgtat cattttttac tattcagtca ttcgtttctt ctttatcgaa tacgtcaacg
     5941 ctcaaaaaca tgctgtatgt tctttcaaca gcagtagaat tcgaaagtgt tccactcaga
     6001 aaaggagaca gagcattact tgtcaaatta agtaaaagac taccattgag attccctgaa
     6061 cacacctctt ctggttcggt gagctttaaa gttttcttat tgttacaagc atatttttca
     6121 cgtcttgaat tacctgtcga ctttcaaaat gatttaaaag acattctaga aaaggttgtt
     6181 ccactaatta acgtagtggt tgatatcctt tcagcaaacg ggtatttgaa tgctactacc
     6241 gccatggatt tggcgcagat gttgattcag ggtgtatggg acgttgataa tccgttgaga
     6301 cagattccac acttcaacaa caaaattttg gaaaaatgta aagaaattaa cgtcgagacg
     6361 gtctatgata taatggccct tgaggatgaa gagagggatg aaattttaac gcttactgat
     6421 tcacagcttg cgcaggtcgc cgcgtttgtt aacaattacc ccaacgttga acttacttat
     6481 tcgttgaata attctgattc gttaatttca ggtgtaaaac agaaaattac cattcaattg
     6541 acaagagatg ttgaacctga gaacttacaa gtaacatctg aaaagtatcc gtttgataag
     6601 ctagaaagtt ggtggttggt tttaggtgaa gtatctaaaa aagagcttta tgcgatcaag
     6661 aaagttactt taaataaaga gactcagcaa tatgaattgg aatttgacac tccgacatct
     6721 ggtaaacata acttaacaat ttggtgtgtc tgtgattcat atcttgacgc agataaagag
     6781 ttgtcctttg aaataaatgt gaaactcgag ggatccatgg aaaagagaag atggaaaaag
     6841 aatttcatag ccgtctcagc agccaaccgc tttaagaaaa tctcatcctc cggggcactt
     6901 gattatgata ttccaactac tgctagcgag aatttgtatt ttcagggtga gctcaaaacc
     6961 gcggctcttg cgcaacacga tgaagccgtg gacaacaaat tcaacaaaga acaacaaaac
     7021 gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa cgccttcatc
     7081 caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc taaaaagcta
     7141 aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagagcaaca aaacgcgttc
     7201 tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt catccaaagt
     7261 ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa gctaaatggt
     7321 gctcaggcgc cgaaagtaga cgcgaattcc gcggggaagt caacctgaag cttgatatcg
     7381 aattcctgca gcccggggga tccactagtt ctagagcggc cgggcccggt acccaattcg
     7441 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     7501 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     7561 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     7621 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7681 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7741 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7801 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7861 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7921 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7981 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     8041 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     8101 ccttacgcat ctgtgcggta tttcacaccg catagatccg tcgagttcaa gagaaaaaaa
     8161 aagaaaaagc aaaaagaaaa aaggaaagcg cgcctcgttc agaatgacac gtatagaatg
     8221 atgcattacc ttgtcatctt cagtatcata ctgttcgtat acatacttac tgacattcat
     8281 aggtatacat atatacacat gtatatatat cgtatgctgc agctttaaat aatcggtgtc
     8341 actacataag aacacctttg gtggagggaa catcgttggt accattgggc gaggtggctt
     8401 ctcttatggc aaccgcaaga gccttgaacg cactctcact acggtgatga tcattcttgc
     8461 ctcgcagaca atcaacgtgg agggtaattc tgctagcctc tgcaaagctt tcaagaaaat
     8521 gcgggatcat ctcgcaagag agatctccta ctttctccct ttgcaaacca agttcgacaa
     8581 ctgcgtacgg cctgttcgaa agatctacca ccgctctgga aagtgcctca tccaaaggcg
     8641 caaatcctga tccaaacctt tttactccac gcacggcccc tagggcctct ttaaaagctt
     8701 gaccgagagc aatcccgcag tcttcagtgg tgtgatggtc gtctatgtgt aagtcaccaa
     8761 tgcactcaac gattagcgac cagccggaat gcttggccag agcatgtatc atatggtcca
     8821 gaaaccctat acctgtgtgg acgttaatca cttgcgattg tgtggcctgt tctgctactg
     8881 cttctgcctc tttttctggg aagatcgagt gctctatcgc taggggacca ccctttaaag
     8941 agatcgcaat ctgaatcttg gtttcatttg taatacgctt tactagggct ttctgctctg
     9001 tcatctttgc cttcgtttat cttgcctgct cattttttag tatattcttc gaagaaatca
     9061 cattacttta tataatgtat aattcattat gtgataatgc caatcgctaa gaaaaaaaaa
     9121 gagtcatccg ctaggtggaa aaaaaaaaat gaaaatcatt accgaggcat aaaaaaatat
     9181 agagtgtact agaggaggcc aagagtaata gaaaaagaaa attgcgggaa aggactgtgt
     9241 tatgacttcc ctgactaatg ccgtgttcaa acgatacctg gcagtgactc ctagcgctca
     9301 ccaagctctt aaaacgggaa tttatggtgc actctcagta caatctgctc tgatgccgca
     9361 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     9421 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     9481 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     9541 taggttaatg tcatgataat aatggtttct tagggtcctt ttcatcacgt gctataaaaa
     9601 taattataat ttaaattttt taatataaat atataaatta aaaatagaaa gtaaaaaaag
     9661 aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa aagactctag ggggatcgcc
     9721 aacaaatact accttttatc ttgctcttcc tgctctcagg tattaatgcc gaattgtttc
     9781 atcttgtctg tgtagaagac cacacacgaa aatcctgtga ttttacattt tacttatcgt
     9841 taatcgaatg tatatctatt taatctgctt ttcttgtcta ataaatatat atgtaaagta
     9901 cgctttttgt tgaaattttt taaacctttg tttatttttt tttcttcatt ccgtaactct
     9961 tctaccttct ttatttactt tctaaaatcc aaatacaaaa cataaaaata aataaacaca
    10021 gagtaaattc ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa gttacaggca
    10081 agcgatccgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    10141 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    10201 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    10261 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    10321 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    10381 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    10441 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
    10501 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    10561 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    10621 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    10681 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    10741 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    10801 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    10861 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    10921 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    10981 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    11041 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    11101 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    11161 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    11221 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    11281 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    11341 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    11401 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    11461 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    11521 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    11581 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    11641 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    11701 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    11761 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    11821 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    11881 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    11941 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    12001 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    12061 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    12121 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
    12181 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
    12241 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctc
    12301 caccgcggtg gcggccgcgg ctgcaggtcg agttta
//
