LOCUS       sequence-178420-        7206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1000-1826.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Schou KB, Mogensen JB, Morthorst SK, Nielsen BS, Aleliunaite A, 
            Serra-Marques A, Furstenberg N, Saunier S, Bizet AA, Veland IR, 
            Akhmanova A, Christensen ST, Pedersen LB
  TITLE     KIF13B establishes a CAV1-enriched microdomain at the ciliary 
            transition zone to promote Sonic hedgehog signalling.
  JOURNAL   Nat Commun. 2017 Jan 30;8:14177. doi: 10.1038/ncomms14177.
  PUBMED    28134340
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-01-30"; volume: "8:14177. doi"; pages: " 
            10.1038/ncomms14177"
COMMENT     Sequence Label: LP711
FEATURES             Location/Qualifiers
     source          1..7206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3934..4055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3971..3990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4025..4044
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4062..4517)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4199..4220)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4411..4430
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4544..4648
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4650..5007
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4650..4841
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      4858..4993
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5042..5836
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5042..5836
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5096..5115)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5706..5725
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6024..6043)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6068..6115
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6444..7032
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6933..6952
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1021 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1321 gagaatggtg aatactgccc tgtagaagtg atttctgcaa aggatgtccc aacaggagga
     1381 atcttccagc tccggcaggg gcagtcccgg agagttcaag tcgaagtgaa gtcagtgcag
     1441 gaatctggga ctttaccact gatggaagaa tgtatactgt ctgttggcat tggatgtgtc
     1501 aaagttagac cgctcagagc ccccagaaca catgagacct tccatgagga agaggaagac
     1561 atggacagct accaggatcg agatttagag agacttcgta gaaaatggct aaatgcatta
     1621 acaaaacgtc aggagtactt ggatcaacaa ttgcaaaagc ttgtcagtaa acgtgataaa
     1681 acagaggatg atgctgaccg cgaagcgcag cttctggaga tgcggttgac cctaactgag
     1741 gagaggaacg cggtgatggt cccctctgct ggcagtggta ttccaggggc cccagcagaa
     1801 tggaccccag tacctgggat ggagacacac attcctgtta tattcctgga cttaaatgct
     1861 gatgatttca gctctcagga taatcttgat gacccagaag ctggtggatg ggatgcgacc
     1921 ttgactgggg aagaagaaga ggagttcttt gaattgcaga ttgtgaagca gcatgatggg
     1981 gaggtgaaag cagaagcctc ctgggactcc gcggtgcatg gctgccctca gctcagcagg
     2041 ggcacgcccg tggacgagcg gttgttcctg atcgtgcgcg tgacggtcca gctcagccac
     2101 cctgctgaca tgcaactggt gttacgcaag agaatctgtg tcaatgttca cggccgccag
     2161 ggttttgcac agagtctcct aaaaaagatg tctcatcgaa gttctattcc tggctgtgga
     2221 gtgacttttg aaattgtctc caatattcca gaggatgccc agggagtgga agaacgggaa
     2281 gcattagcaa gaatggcagc caatgttgaa aacccagctt ctgctgactc ggaggcttat
     2341 attgaaaagt acctcaggag cgtgctggct gtagaaaacc tcctgacttt agatcgtctg
     2401 cgccaggaag ttgcagtgaa ggaacagtta acaggaaaag gaaagttgag caggaggagt
     2461 atcagttctc caaatgtgaa cagattgtct ggaagccgac aagatctcat tccatcatac
     2521 agtctaggca gcaacaaggg ccggtgggaa agtcagcagg atgtatccca aaccacagtt
     2581 tccagaggaa tagctcctgc ccccgccctc tctgtttctc cccaaaataa ccattctcca
     2641 gatccaggac tcagtaacct tgcagcatcc tacttgaatc ctgtcaaatc cttcgtgccg
     2701 caaatgccaa agctcctcaa gtctctcttt cccgtccgcg atgagaagag gggcaagcgg
     2761 ccgtctcccc tcgcacacca gcccgtgccc cgcatcatgg tgcagtcagc cagcccggac
     2821 atcagggtga ccaggatgga ggaggctcag ccggagatgg gccctgacgt gctggtgcag
     2881 acgatggggg ccccggcctt gaagatctgc gacaaacctg ccaaagtgcc ttccccaccg
     2941 cctgtcatag ctgtcacagc ggtcaccccg gctccggagg cacaggacgg gccccccagc
     3001 cccctgagtg aagcctctag cgggtacttc tcccacagcg tctccaccgc gaccctgtcg
     3061 gacgccctgg gccccggcct ggacgctgcg gccccgccgg ggtccatgcc caccgcccct
     3121 gaggccgagc ccgaggcgcc catcagccac cccccaccgc ccacggccgt ccccgccgag
     3181 gagccccctg gcccccagca gctcgtgagc cccggtcggg agcgccccga cctcgaggcc
     3241 ccggcgcccg gctccccgtt ccgcgtccgg agggtgcggg cctcggagtt gcgctccttc
     3301 tcgcgcatgc tggctgggga ccccggctgc tccccggggg ccgaggggaa tgcgccggcc
     3361 ccgggcgccg ggggacaggc cctggcctct gattccgagg aagctgacga ggtcccggag
     3421 tggctccgag agggcgagtt cgtcaccgtg ggcgcccaca aaacgggcgt ggtgagatac
     3481 gtggggcctg ccgacttcca agagggcacg tgggtcggcg tggagctcga cctgccctca
     3541 ggtaagaatg acggttccat cggcgggaag cagtacttca ggtgtaaccc tggctacggg
     3601 ctgctggtca ggcccagccg ggtccgcagg gccacgggcc ctgtgtggcg gcgcagcaca
     3661 ggactccggc tgggtgcccc cgaggcccgc cggagcgcca ccctctcggg ctccgccacc
     3721 aacctggcct cgctgacagc tgccctggcc aaggccgaca ggagccacaa gaaccctgag
     3781 aaccggaaat cctgggccag ctgaggatcc accggatcta gataactgat cataatcagc
     3841 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     3901 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     3961 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4021 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     4081 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     4141 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     4201 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4261 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     4321 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4381 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4441 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4501 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4561 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4621 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     4681 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4741 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4801 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4861 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4921 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4981 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     5041 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     5101 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5161 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5221 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5281 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5341 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5401 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5461 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5521 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     5581 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5641 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5701 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5761 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5821 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5881 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5941 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     6001 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     6061 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     6121 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     6181 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     6241 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     6301 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6361 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6421 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6481 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6541 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6601 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6661 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6721 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6781 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6841 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6901 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6961 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7021 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7081 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7141 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     7201 gttccg
//
