LOCUS       sequence-178423-        8580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1-1289.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Schou KB, Mogensen JB, Morthorst SK, Nielsen BS, Aleliunaite A, 
            Serra-Marques A, Furstenberg N, Saunier S, Bizet AA, Veland IR, 
            Akhmanova A, Christensen ST, Pedersen LB
  TITLE     KIF13B establishes a CAV1-enriched microdomain at the ciliary 
            transition zone to promote Sonic hedgehog signalling.
  JOURNAL   Nat Commun. 2017 Jan 30;8:14177. doi: 10.1038/ncomms14177.
  PUBMED    28134340
REFERENCE   2  (bases 1 to 8580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-01-30"; volume: "8:14177. doi"; pages: " 
            10.1038/ncomms14177"
COMMENT     Sequence Label: LP399
FEATURES             Location/Qualifiers
     source          1..8580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      2294..2303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5308..5429
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5345..5364)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5399..5418
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5436..5891)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5573..5594)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5785..5804
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5918..6022
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6024..6381
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6024..6215
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      6232..6367
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6416..7210
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6416..7210
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6470..6489)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7080..7099
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7398..7417)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7442..7489
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7818..8406
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8307..8326
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1021 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1321 atgggggact ccaaagtgaa agtggcggtg cggatacgac ccatgaaccg gcgagagact
     1381 gacttgcata ccaaatgtgt ggtggatgtg gatgcaaaca aggttattct taatcctgta
     1441 aatacgaatc tttccaaagg agatgcccgg ggccagccga aggtgtttgc ttatgatcat
     1501 tgtttctggt ctatggatga atctgtcaaa gaaaagtatg caggtcaaga tattgttttc
     1561 aagtgccttg gagagaatat cctgcagaat gcttttgatg gctacaatgc atgtatcttt
     1621 gcctatggac agactggctc tggaaaatct tataccatga tgggcacagc tgaccaacct
     1681 ggattaatcc caagactttg cagtggactc tttgaacgaa ctcagaaaga ggaaaatgaa
     1741 gaacagagtt ttaaagtaga agtgtcctac atggaaattt ataatgaaaa agttcgagac
     1801 cttcttgatc ccaaaggaag ccgtcagacg ttgaaagtca gagagcatag tgtgttggga
     1861 ccttatgtcg acggactttc taaactggct gtcacaagct acaaggatat tgagtcgttg
     1921 atgtctgagg gtaacaaatc tcgcacagtt gctgcaacca acatgaacga ggagagtagc
     1981 cgatcccatg cagttttcaa aatcaccctc acacatactc tctacgatgt gaagtctggg
     2041 acatctggag agaaagtggg caaactcagc ctggtggatt tagctggcag tgaacgagca
     2101 acgaagacag gcgctgcagg ggacaggctg aaggaaggga gcaacattaa caagtccctc
     2161 acaaccctcg gtctggttat ctcagctctt gcagatcaga gtgctggcaa aaacaagaat
     2221 aaatttgttc catatcgtga ctcagttctc acttggctgc tcaaagacag cctcgggggt
     2281 aacagcaaga ccgccatggt ggctactgtg agtcctgcag ctgataacta tgatgaaacc
     2341 ctctcaactc tgcggtatgc agatcgagcc aagcacattg taaaccacgc tgtggtgaat
     2401 gaggacccta atgcccgaat tatccgggat ctccgggaag aagttgagaa actccgggag
     2461 cagctgacca aagcagaggc aatgaaatct ccagagctaa aggaccggct ggaagaatct
     2521 gagaagctaa tccaggaaat gactgtgacc tgggaggaga aattaaggaa aacggaggag
     2581 attgcacagg aacgacagaa acagcttgag agtcttggaa tatctcttca gtcttcggga
     2641 atcaaagttg gggatgataa atgcttcctt gtgaatctga atgctgaccc agctctgaat
     2701 gagcttctgg tgtactattt aaaggaacat acattgatag ggtcagcaaa ttcccaagat
     2761 atccaactgt gcggcatggg aattcttcct gaacactgta ttatagacat cacgtcagaa
     2821 ggccaggtta tgctgactcc tcagaagaac accagaacat ttgtaaatgg gtcatctgtc
     2881 tccagtccaa tacagctaca ccatggggac aggatattat ggggaaacaa tcatttcttc
     2941 agactcaatt tgcctaaaaa gaaaaagaaa gcagaacgag aggatgagga ccaggatccc
     3001 tccatgaaga acgagaatag ttctgagcag ctggatgtag acggagactc ctccagcgag
     3061 gtgtccagtg aagttaactt taattacgaa tacgcacaga tggaggtcac catgaaggcc
     3121 ctgggcagca atgatccgat gcagtccata ttaaacagcc tagaacaaca gcatgaagaa
     3181 gaaaaacgat ctgcactgga gcgccagagg cttatgtatg agcacgaatt ggagcagctc
     3241 cggagaaggc tgtctcctga gaagcagaac tgccggagca tggacaggtt ttctttccac
     3301 tcgcccagcg ctcagcaacg cttaagacag tgggctgagg agagagaagc aacgttgaat
     3361 aacagcctga tgaggctgag ggaacaaatt gttaaggcca atctattggt gagagaagct
     3421 aattacattg ctgaggagct ggataaaaga acagaataca aagttaccct acagattcca
     3481 gcctccagcc tggatgccaa caggaagcga ggctctcttc ttagtgagcc tgcaatccag
     3541 gtgagaagaa aaggaaaagg aaagcagatt tggtctttgg aaaaactgga caacaggctg
     3601 ttggatatga gagaccttta tcaggagtgg aaagagtgtg aagaagataa cccagtaata
     3661 cgatcatact tcaaacgtgc tgatccattc tatgatgagc aggaaaatca cagtctcatt
     3721 ggggtggcca atgtcttcct cgagtcactt ttctattatg tgaagttaca atacgctgtt
     3781 cccatcatca accagaaagg agaggtggca ggtcggctgc acgtggaggt gatgcgactc
     3841 agtggtgatg ttggggagag gatcgcagga ggcgatgagg tggcagaggt ctcctttgag
     3901 aaggagaccc aggagaacaa actggtgtgc atggttaaaa tcctgcaagc tactgggttg
     3961 ccacagcatc tgtcccactt tgtgttctgc aaatacagct tctgggatca acaggagccg
     4021 gtgattgtcg ctcctgaagt ggacacctcc tcctcttccg tcagcaagga gccgcactgc
     4081 atggttgtct ttgatcattg caatgagttt tctgttaaca tcaccgaaga ctttatcgag
     4141 catctttccg aaggagcatt ggcaattgaa gtatatggac ataaaataaa cgatccccgg
     4201 aaaaaccccg ccctgtggga tttgggaatc atccaagcaa agacacgtag tcttcgggac
     4261 agatggagtg aagtgaccag gaaattggaa ttctgggttc aaatcttgga acagaatgag
     4321 aatggtgaat actgccctgt agaagtgatt tctgcaaagg atgtcccaac aggaggaatc
     4381 ttccagctcc ggcaggggca gtcccggaga gttcaagtcg aagtgaagtc agtgcaggaa
     4441 tctgggactt taccactgat ggaagaatgt atactgtctg ttggcattgg atgtgtcaaa
     4501 gttagaccgc tcagagcccc cagaacacat gagaccttcc atgaggaaga ggaagacatg
     4561 gacagctacc aggatcgaga tttagagaga cttcgtagaa aatggctaaa tgcattaaca
     4621 aaacgtcagg agtacttgga tcaacaattg caaaagcttg tcagtaaacg tgataaaaca
     4681 gaggatgatg ctgaccgcga agcgcagctt ctggagatgc ggttgaccct aactgaggag
     4741 aggaacgcgg tgatggtccc ctctgctggc agtggtattc caggggcccc agcagaatgg
     4801 accccagtac ctgggatgga gacacacatt cctgttatat tcctggactt aaatgctgat
     4861 gatttcagct ctcaggataa tcttgatgac ccagaagctg gtggatggga tgcgaccttg
     4921 actggggaag aagaagagga gttctttgaa ttgcagattg tgaagcagca tgatggggag
     4981 gtgaaagcag aagcctcctg ggactccgcg gtgcatggct gccctcagct cagcaggggc
     5041 acgcccgtgg acgagcggtt gttcctgatc gtgcgcgtga cggtccagct cagccaccct
     5101 gctgacatgc aactggtgtt acgcaagaga atctgtgtca atgttcacgg ccgccagggt
     5161 tttgcacaga gtctcctaaa aaagatgtag tctagataac tgatcataat cagccatacc
     5221 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     5281 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     5341 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5401 ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt
     5461 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     5521 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     5581 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     5641 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     5701 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     5761 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     5821 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     5881 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5941 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6001 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     6061 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6121 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6181 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6241 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6301 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6361 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     6421 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6481 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6541 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     6601 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6661 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6721 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     6781 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     6841 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6901 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     6961 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     7021 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     7081 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     7141 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7201 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     7261 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     7321 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     7381 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     7441 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     7501 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     7561 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     7621 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     7681 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     7741 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7801 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7861 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7921 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     7981 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8041 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8101 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8161 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8221 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8281 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8341 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8401 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     8461 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
     8521 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
//
