LOCUS       sequence-178425-        6389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pEGFP-N1 with cDNA encoding HsKIF13B aa residues 1-557.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Schou KB, Mogensen JB, Morthorst SK, Nielsen BS, Aleliunaite A, 
            Serra-Marques A, Furstenberg N, Saunier S, Bizet AA, Veland IR, 
            Akhmanova A, Christensen ST, Pedersen LB
  TITLE     KIF13B establishes a CAV1-enriched microdomain at the ciliary 
            transition zone to promote Sonic hedgehog signalling.
  JOURNAL   Nat Commun. 2017 Jan 30;8:14177. doi: 10.1038/ncomms14177.
  PUBMED    28134340
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-01-30"; volume: "8:14177. doi"; pages: " 
            10.1038/ncomms14177"
COMMENT     Sequence Label: LP241
FEATURES             Location/Qualifiers
     source          1..6389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1541..1550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2269..2278
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2275..2277,2278..2280,2281..2994)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2275..2277,2278..2280,2281..2991)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2320..2341)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2581..2600)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2928..2949
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3117..3238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3154..3173)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3208..3227
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3245..3700)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3382..3403)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3594..3613
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3727..3831
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3833..4190
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3833..4024
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3856..3876)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4041..4176
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4225..5019
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4225..5019
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4279..4298)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4889..4908
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5207..5226)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5251..5298
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5627..6215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6116..6135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttatg ggggactcca aagtgaaggt ggcggtgcgg
      601 atacgaccca tgaaccggcg agagactgac ttgcatacca aatgtgtggt ggatgtggat
      661 gcaaacaagg ttattcttaa tcctgtaaat acgaatcttt ccaaaggaga tgcccggggc
      721 cagccgaagg tgtttgctta tgatcattgt ttctggtcta tggatgaatc tgtcaaagaa
      781 aagtatgcag gtcaagatat tgttttcaag tgccttggag agaatatcct gcagaatgct
      841 tttgatggct acaatgcatg tatctttgcc tatggacaga ctggctctgg aaaatcttat
      901 accatgatgg gcacagctga ccaacctgga ttaatcccaa gactttgcag tggactcttt
      961 gaacgaactc agaaagagga aaatgaagaa cagagtttta aagtagaagt gtcctacatg
     1021 gaaatttata atgaaaaagt tcgagacctt cttgatccca aaggaagccg tcagacgttg
     1081 aaagtcagag agcatagtgt gttgggacct tatgtcgacg gactttctaa actggctgtc
     1141 acaagctaca aggatattga gtcgttgatg tctgagggta acaaatctcg cacagttgct
     1201 gcaaccaaca tgaacgagga gagtagccga tcccatgcag ttttcaaaat caccctcaca
     1261 catactctct acgatgtgaa gtctgggaca tctggagaga aagtgggcaa actcagcctg
     1321 gtggatttag ctggcagtga acgagcaacg aagacaggcg ctgcagggga caggctgaag
     1381 gaagggagca acattaacaa gtccctcaca accctcggtc tggttatctc agctcttgca
     1441 gatcagagtg ctggcaaaaa caagaataaa tttgttccat atcgtgactc agttctcact
     1501 tggctgctca aagacagcct cgggggtaac agcaagaccg ccatggtggc tactgtgagt
     1561 cctgcagctg ataactatga tgaaaccctc tcaactctgc ggtatgcaga tcgagccaag
     1621 cacattgtaa accacgctgt ggtgaatgag gaccctaatg cccgaattat ccgggatctc
     1681 cgggaagaag ttgagaaact ccgggagcag ctgaccaaag cagaggcaat gaaatctcca
     1741 gagctaaagg accggctgga agaatctgag aagctaatcc aggaaatgac tgtgacctgg
     1801 gaggagaaat taaggaaaac ggaggagatt gcacaggaac gacagaaaca gcttgagagt
     1861 cttggaatat ctcttcagtc ttcgggaatc aaagttgggg atgataaatg cttccttgtg
     1921 aatctgaatg ctgacccagc tctgaatgag cttctggtgt actatttaaa ggaacataca
     1981 ttgatagggt cagcaaattc ccaagatatc caactgtgcg gcatgggaat tcttcctgaa
     2041 cactgtatta tagacatcac gtcagaaggc caggttatgc tgactcctca gaagaacacc
     2101 agaacatttg taaatgggtc atctgtctcc agtccaatac agctacacca tggggacagg
     2161 atattatggg gaaacaatca tttcttcaga ctcaatttgc ctaaaaagaa aaagaaagca
     2221 gaacgagagg atgaggacac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg
     2281 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     2341 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     2401 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     2461 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     2521 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     2581 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     2641 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2701 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     2761 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     2821 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2881 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     2941 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc
     3001 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     3061 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     3121 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3181 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
     3241 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     3301 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     3361 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     3421 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     3481 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     3541 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     3601 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     3661 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     3721 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3781 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     3841 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     3901 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3961 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4021 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4081 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     4141 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     4201 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     4261 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     4321 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     4381 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     4441 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4501 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     4561 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     4621 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     4681 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     4741 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     4801 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     4861 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     4921 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4981 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     5041 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     5101 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     5161 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     5221 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     5281 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     5341 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     5401 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     5461 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5521 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5581 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5641 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5701 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5761 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5821 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5881 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5941 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6001 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6061 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6121 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6181 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6241 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6301 ctgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca
     6361 ttagttcata gcccatatat ggagttccg
//
