LOCUS       sequence-178427-        4049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CpG free plasmid for testing effects of methylation on regulatory 
            activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Tung
  TITLE     Tung lab mSTARR-seq plasmud
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pmSTARRseq1
FEATURES             Location/Qualifiers
     source          1..4049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          709..938
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     921..940
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             986..1702
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQRNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    2305..2426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2316..2335)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2370..2389
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ttaattaaaa ttatctctaa ggcatgtgaa ctggctgtct tggttttcat ctgtacttca
       61 tctgctacct ctgtgacctg aaacatattt ataattccat taagctgtgc atatgataga
      121 tttatcatat gtattttcct taaaggattt ttgtaagaac taattgaatt gatacctgta
      181 aagtctttat cacactaccc aataaataat aaatctcttt gttcagctct ctgtttctat
      241 aaatatgtac cagttttatt gtttttagtg gtagtgattt tattctcttt ctatatatat
      301 acacacacat gtgtgcattc ataaatatat acaattttta tgaataaaaa attattagca
      361 atcaatattg aaaaccactg atttttgttt atgtgagcaa acagcagatt aaaaggaatt
      421 cctgcagggc ccacctagga tgcataggga gaagagcatg cttgagggct gagtgcccct
      481 cagtgggcag agagcacatg gcccacagtc cctgagaagt tggggggagg ggtgggcaat
      541 tgaactggtg cctagagaag gtggggcttg ggtaaactgg gaaagtgatg tggtgtactg
      601 gctccacctt tttccccagg gtgggggaga accatatata agtgcagtag tctctgtgaa
      661 cattcagggc ctgaattaat tcactgtctg ccagggccag ctgttggggt gagtactccc
      721 tctcaaaagc tggcatgact tctgcactaa gattgtcagt ttccaaaaat gaggaggatt
      781 tgatattcaa ctggccctca gtgatgcctt tgagggtggc tgggtccatc tggtcagaaa
      841 agacaatctt tttgttgtca agcttgaggt gtggcaggct tgaggtctgg ccatacactt
      901 gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc aggtccaact
      961 gcaggtcacc tgcaggctta agctcatggt ttctaaggga gaagaactct ttactggtgt
     1021 tgtcccaatt ctggttgagc tggatggtga tgtgaatggc cacaaattct ctgtgtctgg
     1081 tgaaggtgaa ggagatgcaa cttatggaaa gctgactctg aagttcattt gtacaacagg
     1141 aaagctgcca gtgccttggc caactctggt gaccaccctg acttatggtg ttcaatgttt
     1201 cagcaggtac cctgaccaca tgaagcagca tgacttcttt aaatctgcaa tgccagaagg
     1261 ttatgttcag gagaggacaa tcttctttaa ggatgatgga aattataaga caagggcaga
     1321 agtgaagttt gaaggtgata cactggttaa cagaattgag ctgaaaggca ttgattttaa
     1381 ggaagatgga aacattctgg gtcacaagct ggagtacaac tataattctc acaatgttta
     1441 cattatggca gataagcaga ggaatggaat taaggctaat ttcaagatta gacacaacat
     1501 tgaggatggg tctgtccaac tggcagacca ttaccagcag aacaccccta ttggtgatgg
     1561 cccagttctc ctcccagata atcactatct cagcactcaa tctgctctgt ccaaagaccc
     1621 taatgagaaa agagaccaca tggtcctcct ggagtttgtg acagcagcag gaattactct
     1681 gggaatggat gagctgtaca aggccaagtt gaccagtgct gtcccagtgc tcacagccag
     1741 ggatgtggct ggagctgttg agttctggac tgacaggttg gggttctcca gagattttgt
     1801 ggaggatgac tttgcaggtg tggtcagaga tgatgtcacc ctgttcatct cagcagtcca
     1861 ggaccaggtg gtgcctgaca acaccctggc ttgggtgtgg gtgagaggac tggatgagct
     1921 gtatgctgag tggagtgagg tggtctccac caacttcagg gatgccagtg gccctgccat
     1981 gacagagatt ggagagcagc cctgggggag agagtttgcc ctgagagacc cagcaggcaa
     2041 ctgtgtgcac tttgtggcag aggagcagga ctaaagtcta gagcttgtac tagtggtgat
     2101 tcccctgacg cgtgcacgtc tgctgacgcg taaagtctcc cgtgaacttt acccacgcgt
     2161 gcatatcggg gatgaaagac gcgtcatgat gaccaccgat atggccagtg tacgcgtctc
     2221 ggggaatata aatacacagc cagtctgcag gacatcccat gggaattcag ccagccactt
     2281 caactgctag ctggccagac atgataagat acattgatga gtttggacaa accacaacta
     2341 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2401 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2461 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     2521 aattcagtca atatgttcac cccaaaaaag ctgtttgtta acttgccaac ctcattctaa
     2581 aatgtatata gaagcccaaa agacaataac aaaaatattc ttgtagaaca aaatgggaaa
     2641 gaatgttcca ctaaatatca agatttagag caaagcatga gatgtgtggg gatagacagt
     2701 gaggctgata aaatagagta gagctcagaa acagacccat tgatatatgt aagtgaccta
     2761 tgaaaaaaat atggcatttt acaatgggaa aatgatggtc tttttctttt ttagaaaaac
     2821 agggaaatat atttatatgt aaaaaataaa agggaaccca tatgtcatac catacacaca
     2881 aaaaaattcc agtgaattat aagtctaaat ggagaaggca aaactttaaa tcttttagaa
     2941 aataatatag aagcatgcca tcaagacttc agtgtagaga aaaatttctt atgactcaaa
     3001 gtcctaacca caaagaaaag attgttaatt agattgcatg aatattaaga cttattttta
     3061 aaattaaaaa accattaaga aaagtcaggc catagaatga cagaaaatat ttgcaacacc
     3121 ccagtaaaga gaattgtaat atgcagatta taaaaagaag tcttacaaat cagtaaaaaa
     3181 taaaactaga caaaaatttg aacagatgaa agagaaactc taaataatca ttacacatga
     3241 gaaactcaat ctcagaaatc agagaactat cattgcatat acactaaatt agagaaatat
     3301 taaaaggcta agtaacatct gtggcttaat taaaatcagc agttcaacct gttgatagta
     3361 tgtactaagc tctcatgttt aatgtactaa gctctcatgt ttaatgaact aaaccctcat
     3421 ggctaatgta ctaagctctc atggctaatg tactaagctc tcatgtttca tgtactaagc
     3481 tctcatgttt gaacaataaa attaatataa atcagcaact taaatagcct ctaaggtttt
     3541 aagttttata agaaaaaaaa gaatatataa ggcttttaaa ggttttaagg tttcctagct
     3601 ttagtcctgt tcctcagcta caaaatggac acaatttcca gcagggtctc tgagggcaaa
     3661 ttcccttccc caaggttgtt caccaatttc tgtcatggct gggccagagg catccctgaa
     3721 atttgtgctg actacttctg accattctgc ataaagctca tctaggcctc tgacccagac
     3781 ccaagcaagg gtgttgtcag ggacaacttg gtcctgaact gctgagatga agagggtgac
     3841 atcatctctg acaacaccag caaaatcatc ttcaacaaag tctctggaga atcctaatct
     3901 gtcagtccag aactctacag cccctgcaac atcccttgct gtgaggactg ggactgcaga
     3961 agtgagtttg gccatgatgg ccctcctata gtgagttgta ttatactatg cagatatact
     4021 atgccaatgt ttaattgtca actactgtt
//
