LOCUS       sequence-178432-        7713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1-1000.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Schou KB, Mogensen JB, Morthorst SK, Nielsen BS, Aleliunaite A, 
            Serra-Marques A, Furstenberg N, Saunier S, Bizet AA, Veland IR, 
            Akhmanova A, Christensen ST, Pedersen LB
  TITLE     KIF13B establishes a CAV1-enriched microdomain at the ciliary 
            transition zone to promote Sonic hedgehog signalling.
  JOURNAL   Nat Commun. 2017 Jan 30;8:14177. doi: 10.1038/ncomms14177.
  PUBMED    28134340
REFERENCE   2  (bases 1 to 7713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-01-30"; volume: "8:14177. doi"; pages: " 
            10.1038/ncomms14177"
COMMENT     Sequence Label: LP346
FEATURES             Location/Qualifiers
     source          1..7713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      2294..2303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4441..4562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4478..4497)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4532..4551
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4569..5024)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4706..4727)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4918..4937
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5051..5155
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5157..5514
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5157..5348
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5180..5200)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5365..5500
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5549..6343
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5549..6343
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5603..5622)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6213..6232
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6531..6550)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6575..6622
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6951..7539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7440..7459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1021 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1321 atgggggact ccaaagtgaa agtggcggtg cggatacgac ccatgaaccg gcgagagact
     1381 gacttgcata ccaaatgtgt ggtggatgtg gatgcaaaca aggttattct taatcctgta
     1441 aatacgaatc tttccaaagg agatgcccgg ggccagccga aggtgtttgc ttatgatcat
     1501 tgtttctggt ctatggatga atctgtcaaa gaaaagtatg caggtcaaga tattgttttc
     1561 aagtgccttg gagagaatat cctgcagaat gcttttgatg gctacaatgc atgtatcttt
     1621 gcctatggac agactggctc tggaaaatct tataccatga tgggcacagc tgaccaacct
     1681 ggattaatcc caagactttg cagtggactc tttgaacgaa ctcagaaaga ggaaaatgaa
     1741 gaacagagtt ttaaagtaga agtgtcctac atggaaattt ataatgaaaa agttcgagac
     1801 cttcttgatc ccaaaggaag ccgtcagacg ttgaaagtca gagagcatag tgtgttggga
     1861 ccttatgtcg acggactttc taaactggct gtcacaagct acaaggatat tgagtcgttg
     1921 atgtctgagg gtaacaaatc tcgcacagtt gctgcaacca acatgaacga ggagagtagc
     1981 cgatcccatg cagttttcaa aatcaccctc acacatactc tctacgatgt gaagtctggg
     2041 acatctggag agaaagtggg caaactcagc ctggtggatt tagctggcag tgaacgagca
     2101 acgaagacag gcgctgcagg ggacaggctg aaggaaggga gcaacattaa caagtccctc
     2161 acaaccctcg gtctggttat ctcagctctt gcagatcaga gtgctggcaa aaacaagaat
     2221 aaatttgttc catatcgtga ctcagttctc acttggctgc tcaaagacag cctcgggggt
     2281 aacagcaaga ccgccatggt ggctactgtg agtcctgcag ctgataacta tgatgaaacc
     2341 ctctcaactc tgcggtatgc agatcgagcc aagcacattg taaaccacgc tgtggtgaat
     2401 gaggacccta atgcccgaat tatccgggat ctccgggaag aagttgagaa actccgggag
     2461 cagctgacca aagcagaggc aatgaaatct ccagagctaa aggaccggct ggaagaatct
     2521 gagaagctaa tccaggaaat gactgtgacc tgggaggaga aattaaggaa aacggaggag
     2581 attgcacagg aacgacagaa acagcttgag agtcttggaa tatctcttca gtcttcggga
     2641 atcaaagttg gggatgataa atgcttcctt gtgaatctga atgctgaccc agctctgaat
     2701 gagcttctgg tgtactattt aaaggaacat acattgatag ggtcagcaaa ttcccaagat
     2761 atccaactgt gcggcatggg aattcttcct gaacactgta ttatagacat cacgtcagaa
     2821 ggccaggtta tgctgactcc tcagaagaac accagaacat ttgtaaatgg gtcatctgtc
     2881 tccagtccaa tacagctaca ccatggggac aggatattat ggggaaacaa tcatttcttc
     2941 agactcaatt tgcctaaaaa gaaaaagaaa gcagaacgag aggatgagga ccaggatccc
     3001 tccatgaaga acgagaatag ttctgagcag ctggatgtag acggagactc ctccagcgag
     3061 gtgtccagtg aagttaactt taattacgaa tacgcacaga tggaggtcac catgaaggcc
     3121 ctgggcagca atgatccgat gcagtccata ttaaacagcc tagaacaaca gcatgaagaa
     3181 gaaaaacgat ctgcactgga gcgccagagg cttatgtatg agcacgaatt ggagcagctc
     3241 cggagaaggc tgtctcctga gaagcagaac tgccggagca tggacaggtt ttctttccac
     3301 tcgcccagcg ctcagcaacg cttaagacag tgggctgagg agagagaagc aacgttgaat
     3361 aacagcctga tgaggctgag ggaacaaatt gttaaggcca atctattggt gagagaagct
     3421 aattacattg ctgaggagct ggataaaaga acagaataca aagttaccct acagattcca
     3481 gcctccagcc tggatgccaa caggaagcga ggctctcttc ttagtgagcc tgcaatccag
     3541 gtgagaagaa aaggaaaagg aaagcagatt tggtctttgg aaaaactgga caacaggctg
     3601 ttggatatga gagaccttta tcaggagtgg aaagagtgtg aagaagataa cccagtaata
     3661 cgatcatact tcaaacgtgc tgatccattc tatgatgagc aggaaaatca cagtctcatt
     3721 ggggtggcca atgtcttcct cgagtcactt ttctattatg tgaagttaca atacgctgtt
     3781 cccatcatca accagaaagg agaggtggca ggtcggctgc acgtggaggt gatgcgactc
     3841 agtggtgatg ttggggagag gatcgcagga ggcgatgagg tggcagaggt ctcctttgag
     3901 aaggagaccc aggagaacaa actggtgtgc atggttaaaa tcctgcaagc tactgggttg
     3961 ccacagcatc tgtcccactt tgtgttctgc aaatacagct tctgggatca acaggagccg
     4021 gtgattgtcg ctcctgaagt ggacacctcc tcctcttccg tcagcaagga gccgcactgc
     4081 atggttgtct ttgatcattg caatgagttt tctgttaaca tcaccgaaga ctttatcgag
     4141 catctttccg aaggagcatt ggcaattgaa gtatatggac ataaaataaa cgatccccgg
     4201 aaaaaccccg ccctgtggga tttgggaatc atccaagcaa agacacgtag tcttcgggac
     4261 agatggagtg aagtgaccag gaaattggaa ttctgggttc aaatcttgga acagaatgag
     4321 tagtctagat aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta
     4381 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     4441 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4501 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4561 taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     4621 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     4681 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     4741 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     4801 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     4861 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     4921 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     4981 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     5041 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5101 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
     5161 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5221 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5281 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5341 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5401 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5461 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
     5521 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     5581 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5641 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5701 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
     5761 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     5821 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     5881 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     5941 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6001 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6061 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6121 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6181 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6241 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6301 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6361 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     6421 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6481 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
     6541 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     6601 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     6661 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     6721 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     6781 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
     6841 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6901 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6961 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7021 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7081 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7141 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7201 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7261 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7321 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7381 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7441 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7501 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7561 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7621 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg
     7681 gtcattagtt catagcccat atatggagtt ccg
//
