LOCUS       sequence-178435-        9984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pEGFP-C1 with cDNA encoding HsDLEC1 full length.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9984)
  AUTHORS   Verdier P, Morthorst SK, Pedersen LB
  TITLE     Targeting of ASH Domain-Containing Proteins to the Centrosome.
  JOURNAL   Methods Mol Biol. 2016;1454:15-33. doi: 10.1007/978-1-4939-3789-9_2.
  PUBMED    27514913
REFERENCE   2  (bases 1 to 9984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1454:15-33. doi"; pages: " 
            10.1007/978-1-4939-3789-9_2"
COMMENT     Sequence Label: LP380
FEATURES             Location/Qualifiers
     source          1..9984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6712..6833
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6749..6768)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6803..6822
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6840..7295)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6977..6998)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7189..7208
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7322..7426
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7428..7785
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7428..7619
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7451..7471)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7636..7771
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7820..8614
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7820..8614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7874..7893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8484..8503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8802..8821)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8846..8893
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9222..9810
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9711..9730
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1021 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacatggag
     1321 accaggagct ccaaaacgcg gaggtcttta gcgtcccgga ccaacgagtg ccaggggaca
     1381 atgtgggcgc caacttcgcc accagccggg tccagcagcc ccagccagcc cacctggaag
     1441 tcctccttgt attcctccct cgcctactct gaggccttcc actacagctt cgcagcccgg
     1501 ccccgccgcc tcacgcagct tgcgctggcg cagcgtcccg agcctcagct gcttcgtctg
     1561 cgcccctcct cgctgcgcac ccaagatatc tcgcacttgc tcaccggcgt cttccgcaac
     1621 ttgtactcag ccgaggtcat cggcgacgaa gtgagcgcaa gcttgatcaa ggcccgcggc
     1681 agcgagaatg agcgccacga ggagttcgtg gaccagctgc agcagattcg ggagctctat
     1741 aagcagcggc tggatgagtt tgaaatgttg gagagacata tcactcaggc ccaagcacgg
     1801 gctattgcgg aaaatgagcg ggtcatgagc caggctggag tacaggacct cgagagcctt
     1861 gtcaggttgc ctccagtgaa gagtgtctcc agatggtgta tagacagcga gttgctacgg
     1921 aaacatcatt tgatctcccc agaagattac tacaccgata cagtgccgtt tcactctgca
     1981 cctaaaggca tctccctacc tggatgttca aaactgacat ttagctgtga gaagcgttcc
     2041 gtccagaaga aagagctgaa caagaagctt gaagattcat gcaggaagaa gcttgctgag
     2101 ttcgaagatg agttagacca cactgtggac agcctgacat ggaatttaac tcctaaggcc
     2161 aaagaaagga ccagagaacc tctcaagaaa gcaagtcaac caaggaataa aaactggatg
     2221 aaccacttac gtgtgccaca gagagagcta gacagacttc tgcttgccag aatggagagt
     2281 cggaaccact tcctaaaaaa tccccgtttt tttcctccta acactcgata tggaggcaag
     2341 tctcttgttt ttcctccaaa gaagccagca ccgataggag aattccagag tacagagcca
     2401 gaacagagtt gtgctgatac tccagtgttt ctagctaagc caccaattgg gtttttcaca
     2461 gattatgaaa ttggtccagt ttatgagatg gtaattgcgc tgcagaacac caccacgacc
     2521 agccgctacc tgcgagtcct cccgccttcc acgccatact tcgctctggg actggggatg
     2581 ttcccaggaa aaggtggaat ggtggctcct ggaatgacct gccagtacat tgtccagttt
     2641 tttcccgact gccttgggga ttttgatgat tttattttag tggagaccca gtcagcccac
     2701 acacttctga tccccctgca ggcccggagg ccgccccccg tgctgacatt gtcaccggtg
     2761 ttggactgtg gttactgcct cattggggga gtcaagatga ccagattcat ctgcaaaaat
     2821 gtgggtttca gtgttggcag gttctgcatt atgcccaaaa caagctggcc accactaagt
     2881 ttcaaggcca ttgcaaccgt cggctttgtt gaacaacctc cttttggaat cctgccttcg
     2941 gtgtttgagc tggccccggg acatgctata ttagtggagg tcttgttttc cccaaagagc
     3001 ctaggaaagg cagagcagac cttcatcatc atgtgcgaca actgccagat aaaggagctg
     3061 gtgaccatag gaattgggca gctgattgct ttggatctga tctatatttc tggtgaaaaa
     3121 agccagccag accctggaga gctcacagac ttaacagccc agcacttcat acgatttgag
     3181 cctgaaaacc ttcggtccac ggctaggaag cagctgatta ttagaaatgc tacgcacgtg
     3241 gagctggcct tctactggca gatcatgaag cccaacctgc agcccctcat gcctggagaa
     3301 accttcagca tggacagcat caagtgctac cccgacaagg agactgcctt ctccatcatg
     3361 cccagaaagg gggttctaag cccccacaca gaccacgagt tcatcctgag cttttctcct
     3421 catgagctga gggattttca cagtgtgctc cagatggtgc tagaggaagt cccagagcct
     3481 gtaagttcag aagcggagag cctggggcac tcctcctact ctgtggatga tgtgattgtc
     3541 ctggaaatcg aggtgaaagg ctcagtagaa cctttccagg ttctcttaga gccatatgcc
     3601 ctcatcatcc caggggagaa ctacattggg ataaatgtga agaaggcttt taagatgtgg
     3661 aacaacagca agtcacccat cagatacctg tgggggaaga tcagcgactg ccacatcatt
     3721 gaagtggagc ccggcacagg ggtcatagag cccagtgagg tcggggattt tgagttgaac
     3781 tttactgggg gtgtccctgg ccccacaagc caggacctgc tgtgtgaaat cgaagactcg
     3841 ccctcgccag tggtgttaca cattgaggct gtctttaagg ggcctcccct catcatcaac
     3901 gtctcagccc ttcagtttgg tctgctccgc ctggggcaga aagccacaaa ctccatccag
     3961 atccggaacg tcagccagct cccagccaca tggcgcatga aggagagccc agtctccctc
     4021 caggaaaggc ctgaggatgt gtctcccttc gacattgagc cttcgagtgg ccagcttcac
     4081 tctctggggg agtgcagggt ggacatcacc ttggaggccc tgcactgcca gcatctggag
     4141 accgtcctgg agctggaggt ggaaaatggt gcctggagct accttcctgt gtatgctgag
     4201 gtacagaagc cccatgtgta cctacagagc agccaggtgg aggttagaaa tctctacctg
     4261 ggtgtgccca cgaagacaac catcacactt atcaatggca cgctcctgcc tacccagttc
     4321 cactggggca agctcctcgg acaccaagca gaattctgca tggtgacagt ctcccccaaa
     4381 catggcctgc tgggcccaag tgaggagtgc cagctcaagt tggagttgac tgctcatacc
     4441 caggaagagc tgacccatct ggccctccct tgtcacgtgt caggcatgaa gaagccactg
     4501 gttctaggca tttctgggaa gccccaggga ctgcaagtgg ccattaccat ctctaaggag
     4561 agctctgatt gcagcacaga gcagtggcca ggccacccaa aggagctccg cctggacttt
     4621 ggctcagcgg tgccactgag gacccgtgtg actcgccagc tcattctcac caatcgctcc
     4681 ccaatacgga cccgtttctc cctcaagttt gagtatttcg ggagccccca aaacagcctg
     4741 agcaaaaaga ccagccttcc caacatgcct cctgccctgc taaagacagt gcggatgcaa
     4801 gagcacctgg ccaagcgaga gcagctggat tttatggaga gcatgctatc ccacgggaaa
     4861 ggagctgctt tcttccctca cttttcccag ggcatgctgg ggccctacca gcagctgtgc
     4921 attgacatca caggctgtgc caacatgtgg ggcgagtact gggacaacct catctgcacg
     4981 gtgggagacc tgctgccgga agtcatccca gtgcacatgg cagcggtggg ctgccccatc
     5041 agctccctga ggaccacctc ctacactatt gaccaggccc agaaggaacc agccatgagg
     5101 ttcggcaccc aggtctccgg aggagacaca gttacccgaa cccttcgcct gaataactcc
     5161 agcccctgtg acatccgcct ggattgggag acctatgttc cagaagacaa ggaagaccgg
     5221 ctggtggagc tgctggtgtt ttatgggcca cctttcccgc tgcgggacca agccgggaat
     5281 gagcttgtgt gccctgatac ccctgagggt ggctgcctcc tctggtcccc aggcccctcc
     5341 agttcatcgg aattcagcca tgaaactgac tcatcagttg agggcagctc cagtgccagc
     5401 aatagggtgg cacagaagct catctcagtc atcctgcagg cacatgaggg ggtgccctcc
     5461 ggccacctgt actgtatcag ccccaagcag gtggtggtcc ctgctggggg cagcagtacc
     5521 atctacatct ccttcacccc tatggtgctc agccctgaga tcctgcacaa ggtggagtgt
     5581 actggctacg ccctgggttt catgagcttg gacagcaagg tggaaaggga gattccaggg
     5641 aagaggcatc gcctgcagga ctttgcggtg ggacccctga aactggacct gcatagctac
     5701 gtgaggcctg cacagctaag tgtggagctg gactacggcg gcagtatgga attccagtgc
     5761 caggccagtg acctcattcc cgagcagccc tgctctgggg tgctgagtga gctggtgacc
     5821 acccaccacc tgaagctgac caacactaca gagatcccac actacttccg gcttatggtc
     5881 tccaggccct tctccgtttc tcaagatggg gcgagccagg accacagagc tcctggccct
     5941 ggccagaagc aggagtgtga ggaggagaca gcctcagcgg acaagcagct ggtgctccaa
     6001 gcacaggaga acatgctggt gaacgtgtcc ttctcactct ccctggagct gctctcctat
     6061 cagaagctcc cagctgacca gacactgcct ggggtggaca ttcagcagag tgcgagtgga
     6121 gagagagaga tggtgtttac tcagaacctg ctcctggagt acaccaacca gaccactcag
     6181 gtggtgcccc tgcgggctgt ggtggccgtg cctgagctgc agctctccac cagctgggtg
     6241 gactttggga cctgctttgt gagccagcag cgagtccggg aggtctacct gatgaacctg
     6301 agcgggtgcc gaagctactg gactatgctg atgggccagc aggagccagc caaggccgct
     6361 gtggccttca gggtctcccc aaacagtggg ctgctagaag cacgatccgc caatgcaccc
     6421 ccaacctcca tcgccttgca ggttttcttc actgccagga gtagtgagct gtacgagtcc
     6481 acgatggtgg tggaaggtgt gctcggtgag aagtcctgca ccctgcggct ccggggccaa
     6541 ggctcctatg atgagagata catgttgcct caccagccct gaggatccac cggatctaga
     6601 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     6661 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     6721 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6781 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
     6841 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     6901 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     6961 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     7021 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     7081 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     7141 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     7201 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     7261 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     7321 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7381 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     7441 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     7501 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7561 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7621 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7681 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7741 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     7801 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     7861 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     7921 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     7981 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     8041 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     8101 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     8161 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     8221 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     8281 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     8341 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     8401 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     8461 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     8521 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     8581 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     8641 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     8701 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     8761 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     8821 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     8881 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     8941 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     9001 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     9061 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9121 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9181 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9241 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9301 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9361 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9421 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9481 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9541 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9601 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9661 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9721 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9781 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9841 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     9901 gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt
     9961 tcatagccca tatatggagt tccg
//
