LOCUS       sequence-178438-        7870 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesion Kinase, Excitation: 400 / 490, 
            Emission: 468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7870)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-FAK-5
FEATURES             Location/Qualifiers
     source          1..7870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1266
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    4598..4719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4635..4654)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4689..4708
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4726..5181)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4863..4884)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5075..5094
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5208..5312
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5314..5671
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5314..5505
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5337..5357)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5522..5657
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5706..6500
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5706..6500
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5760..5779)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6370..6389
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6688..6707)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6732..6779
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7108..7696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7597..7616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgagcaa gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc
      601 gagctgaatg gcgatgtgaa tggccacaag ttcagcgtga gcggcgaggg cgagggcgat
      661 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcctgtgccc
      721 tggcccaccc tggtggccac cctgagctac ggcgtgcagt gcttctcacg ctaccccgat
      781 cacatgaagc agcacgactt cttcaagagc gccatgcctg agggctacat ccaggagcgc
      841 accatcttct tcgaggatga cggcaactac aagacgcgcg ccgaggtgaa gttcgagggc
      901 gataccctgg tgagtcgcat cgagctgacc ggcactgatt tcaaggagga tggcaacatc
      961 ctgggcaata agatggagta caactacaac gccaccaatg tgtacatcgt ggccgacaag
     1021 gccaggaatg gcatcaaggt gaacttcaag gtccgccaca acatcaagga tggcagcgtg
     1081 cagctggccg accactacca gcagaatacc cccatcggcg atggccctgt gctgctgccc
     1141 gataaccact acctgtccac ccagagcgcc ctgtccaagg accccaacga gaagcgcgat
     1201 cacatgatct acctcgagtt cgtgaccgcc gccgccatca cccacggcat ggatgagctg
     1261 tacaagtccg gactcagatc tatggcagca gcttaccttg atccaaactt gaatcataca
     1321 ccaagttcaa gtgcaaagac gcacctcggt actgggatgg agcgttcccc gggggccatg
     1381 gagcgagtcc taaaggtttt tcactacttt gaaaacagca gcgagccaac gacgtgggcc
     1441 agcattatcc ggcatggaga tgctactgat gttcgaggca taatacagaa gattgtggac
     1501 tgtcacaaag tgaaaaatgt ggcctgctat gggttgcgac tcagtcatct gcagtctgag
     1561 gaggttcact ggctgcacct ggacatgggg gtatccaatg tgagagagaa atttgaacta
     1621 gcacatcctc cagaagaatg gaaatatgaa ctgagaattc ggtacctgcc caaaggattt
     1681 ctaaaccagt tcactgagga caaaccaact ttaaattttt tctatcagca ggtgaaaaat
     1741 gactatatgt tagaaatagc agatcaagtg gaccaggaaa ttgctttgaa actaggttgc
     1801 cttgaaatca ggagatccta cggagagatg agaggcaatg cattagagaa gaaatccaac
     1861 tatgaagtgc tagaaaaaga tgtcggttta agacgatttt ttccgaagag tttgctagat
     1921 tcagtgaagg ccaaaacact acgaaaatta atccaacaga catttcgaca atttgccaac
     1981 ctcaacagag aagaaagtat tttgaaattc tttgagatcc tctctccagt gtacagattt
     2041 gacaaggaat gcttcaagtg tgcccttggt tcaagctgga ttatttcagt ggagctggca
     2101 attggcccag aggaaggaat cagctacctt acagacaagg gtgcaaatcc aactcacctg
     2161 gcagatttta atcaagtaca aactattcag tattcaaaca gtgaagacaa ggacagaaaa
     2221 gggatgttgc aactgaagat agctggtgca cctgagcctc tgacagtgac agcaccatcc
     2281 ttaaccattg cagagaatat ggctgacttg atagacggat actgccgact ggtgaatgga
     2341 gccacgcaat cttttattat caggccacag aaagaaggtg aaagagcttt accatcaata
     2401 ccaaagctgg ccaacaatga gaagcaagga gtaaggtcgc acacagtctc tgtatcagaa
     2461 acagatgact atgcagagat aatagatgaa gaagatactt atacaatgcc atcaaccaga
     2521 gattatgaaa ttcaaaggga gagaattgaa ctggggcgct gcattggtga aggacagttt
     2581 ggagatgtgc accaaggaat ttacatgagt ccggaaaatc cagctatggc tgtagcaatc
     2641 aaaacatgta aaaactgcac ctcagacagc gttagagaaa agttcctaca agaagcctta
     2701 acaatgcgtc agtttgatca tcctcacatt gtgaagctca ttggagttat tacagaaaac
     2761 ccagtgtgga taatcatgga gctctgtaca cttggagagt tgagatcgtt tctgcaagta
     2821 agaaaattca gcttggacct ggcctccctc atcctctacg cttaccagct tagcacagca
     2881 cttgcttacc tagagagcaa aagatttgta catagagata ttgctgctag gaacgtgctg
     2941 gtatctgcca ctgactgtgt gaaattgggt gactttggct tatcccgata catggaagac
     3001 agtacttact ataaagcttc caaaggaaag ttacctatca aatggatggc tccagagtca
     3061 atcaacttcc gacggtttac ctcagcaagc gatgtgtgga tgtttggtgt gtgtatgtgg
     3121 gagatcctga tgcatggggt aaagcccttc cagggagtga aaaataatga tgttattggt
     3181 cggattgaga acggtgagcg gctccccatg cctccgaact gccctcccac cctctacagc
     3241 cttatgacca agtgctgggc atacgaccct agtagacgac ccaggtttac tgaacttaaa
     3301 gcacaactca gtacaatact ggaggaggag aagctgcagc aagaggaacg aatgagaatg
     3361 gaatccaggc gacaagtcac agtatcctgg gactcaggag gatcagatga agctcctccc
     3421 aagcccagca ggcctggtta ccccagccca aggtccagtg aagggtttta tccgagtcct
     3481 cagcatatgg tacagccaaa tcactaccag gtatctggct actctggttc tcatgggata
     3541 ccagccatgg caggcagcat ttatcctggg caagcttctc tcttggatca aacagattcc
     3601 tggaaccatc gacctcagga agtatcagca tggcagccaa acatggagga ttcgggcact
     3661 ttggatgtac gaggaatggg gcaggttctg cccacacatc tcatggagga gaggttaata
     3721 agacaacagc aagagatgga agaagatcaa cgctggcttg agaaagagga acgattcctg
     3781 gtaatgaaac ctgatgtgcg gctctccaga ggcagcattg aacgggagga cggaggtctc
     3841 cagggcccag ctggtaacca gcacatatat cagcctgtgg gtaaaccaga tcatgccgct
     3901 ccaccaaaga agccccctcg ccctggagcc ccccacttgg gcagcctcgc gagcctgaac
     3961 agccccgtgg acagctacaa cgaaggcgtg aagatcaagc cacaggaaat cagccctcct
     4021 cctacggcca acctggaccg ctccaatgac aaagtctatg agaatgtaac cgggctggtg
     4081 aaagctgtca tagagatgtc cagtaaaata cagccagctc cgccagagga gtacgtgccc
     4141 atggtaaagg aggttggctt ggcgctgaga accttgctag caacagtgga tgagtcgctg
     4201 ccagtgcttc ctgcaagcac ccacagagag attgagatgg cccagaaact gctgaactct
     4261 gacctggctg agctcattaa caagatgaag ctggcccagc agtacgtcat gaccagcctg
     4321 cagcaggagt acaagaagca aatgctgacg gctgctcacg ctctggctgt ggatgccaag
     4381 aacttgctgg atgtcatcga tcaagccaga ctgaaaatga tcagccagtc caggcccact
     4441 aagctgccgt agccgaattc tgcagatggg atccaccgga tctagataac tgatcataat
     4501 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     4561 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     4621 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4681 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta
     4741 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     4801 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     4861 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     4921 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     4981 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     5041 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     5101 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     5161 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5221 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5281 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     5341 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5401 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     5461 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     5521 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5581 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5641 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     5701 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     5761 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     5821 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     5881 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     5941 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     6001 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     6061 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     6121 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     6181 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     6241 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     6301 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     6361 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     6421 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     6481 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     6541 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     6601 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     6661 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     6721 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     6781 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     6841 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     6901 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     6961 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7021 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7081 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7141 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7201 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7261 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7321 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7381 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7441 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7501 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7561 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7621 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7681 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7741 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7801 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     7861 tggagttccg
//
