LOCUS       sequence-178441-        7686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pEGFP-C1 with cDNA encoding HsKIF13B aa residues 840-1826.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Schou KB, Mogensen JB, Morthorst SK, Nielsen BS, Aleliunaite A, 
            Serra-Marques A, Furstenberg N, Saunier S, Bizet AA, Veland IR, 
            Akhmanova A, Christensen ST, Pedersen LB
  TITLE     KIF13B establishes a CAV1-enriched microdomain at the ciliary 
            transition zone to promote Sonic hedgehog signalling.
  JOURNAL   Nat Commun. 2017 Jan 30;8:14177. doi: 10.1038/ncomms14177.
  PUBMED    28134340
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-01-30"; volume: "8:14177. doi"; pages: " 
            10.1038/ncomms14177"
COMMENT     Sequence Label: LP712
FEATURES             Location/Qualifiers
     source          1..7686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4414..4535
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4451..4470)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4505..4524
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4542..4997)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4679..4700)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4891..4910
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5024..5128
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5130..5487
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5130..5321
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      5338..5473
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5522..6316
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5522..6316
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5576..5595)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6186..6205
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6504..6523)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6548..6595
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6924..7512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7413..7432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1021 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1321 ctcagtggtg atgttgggga gaggatcgca ggaggcgatg aggtggcaga ggtctccttt
     1381 gagaaggaga cccaggagaa caaactggtg tgcatggtta aaatcctgca agctactggg
     1441 ttgccacagc atctgtccca ctttgtgttc tgcaaataca gcttctggga tcaacaggag
     1501 ccggtgattg tcgctcctga agtggacacc tcctcctctt ccgtcagcaa ggagccgcac
     1561 tgcatggttg tctttgatca ttgcaatgag ttttctgtta acatcaccga agactttatc
     1621 gagcatcttt ccgaaggagc attggcaatt gaagtatatg gacataaaat aaacgatccc
     1681 cggaaaaacc ccgccctgtg ggatttggga atcatccaag caaagacacg tagtcttcgg
     1741 gacagatgga gtgaagtgac caggaaattg gaattctggg ttcaaatctt ggaacagaat
     1801 gagaatggtg aatactgccc tgtagaagtg atttctgcaa aggatgtccc aacaggagga
     1861 atcttccagc tccggcaggg gcagtcccgg agagttcaag tcgaagtgaa gtcagtgcag
     1921 gaatctggga ctttaccact gatggaagaa tgtatactgt ctgttggcat tggatgtgtc
     1981 aaagttagac cgctcagagc ccccagaaca catgagacct tccatgagga agaggaagac
     2041 atggacagct accaggatcg agatttagag agacttcgta gaaaatggct aaatgcatta
     2101 acaaaacgtc aggagtactt ggatcaacaa ttgcaaaagc ttgtcagtaa acgtgataaa
     2161 acagaggatg atgctgaccg cgaagcgcag cttctggaga tgcggttgac cctaactgag
     2221 gagaggaacg cggtgatggt cccctctgct ggcagtggta ttccaggggc cccagcagaa
     2281 tggaccccag tacctgggat ggagacacac attcctgtta tattcctgga cttaaatgct
     2341 gatgatttca gctctcagga taatcttgat gacccagaag ctggtggatg ggatgcgacc
     2401 ttgactgggg aagaagaaga ggagttcttt gaattgcaga ttgtgaagca gcatgatggg
     2461 gaggtgaaag cagaagcctc ctgggactcc gcggtgcatg gctgccctca gctcagcagg
     2521 ggcacgcccg tggacgagcg gttgttcctg atcgtgcgcg tgacggtcca gctcagccac
     2581 cctgctgaca tgcaactggt gttacgcaag agaatctgtg tcaatgttca cggccgccag
     2641 ggttttgcac agagtctcct aaaaaagatg tctcatcgaa gttctattcc tggctgtgga
     2701 gtgacttttg aaattgtctc caatattcca gaggatgccc agggagtgga agaacgggaa
     2761 gcattagcaa gaatggcagc caatgttgaa aacccagctt ctgctgactc ggaggcttat
     2821 attgaaaagt acctcaggag cgtgctggct gtagaaaacc tcctgacttt agatcgtctg
     2881 cgccaggaag ttgcagtgaa ggaacagtta acaggaaaag gaaagttgag caggaggagt
     2941 atcagttctc caaatgtgaa cagattgtct ggaagccgac aagatctcat tccatcatac
     3001 agtctaggca gcaacaaggg ccggtgggaa agtcagcagg atgtatccca aaccacagtt
     3061 tccagaggaa tagctcctgc ccccgccctc tctgtttctc cccaaaataa ccattctcca
     3121 gatccaggac tcagtaacct tgcagcatcc tacttgaatc ctgtcaaatc cttcgtgccg
     3181 caaatgccaa agctcctcaa gtctctcttt cccgtccgcg atgagaagag gggcaagcgg
     3241 ccgtctcccc tcgcacacca gcccgtgccc cgcatcatgg tgcagtcagc cagcccggac
     3301 atcagggtga ccaggatgga ggaggctcag ccggagatgg gccctgacgt gctggtgcag
     3361 acgatggggg ccccggcctt gaagatctgc gacaaacctg ccaaagtgcc ttccccaccg
     3421 cctgtcatag ctgtcacagc ggtcaccccg gctccggagg cacaggacgg gccccccagc
     3481 cccctgagtg aagcctctag cgggtacttc tcccacagcg tctccaccgc gaccctgtcg
     3541 gacgccctgg gccccggcct ggacgctgcg gccccgccgg ggtccatgcc caccgcccct
     3601 gaggccgagc ccgaggcgcc catcagccac cccccaccgc ccacggccgt ccccgccgag
     3661 gagccccctg gcccccagca gctcgtgagc cccggtcggg agcgccccga cctcgaggcc
     3721 ccggcgcccg gctccccgtt ccgcgtccgg agggtgcggg cctcggagtt gcgctccttc
     3781 tcgcgcatgc tggctgggga ccccggctgc tccccggggg ccgaggggaa tgcgccggcc
     3841 ccgggcgccg ggggacaggc cctggcctct gattccgagg aagctgacga ggtcccggag
     3901 tggctccgag agggcgagtt cgtcaccgtg ggcgcccaca aaacgggcgt ggtgagatac
     3961 gtggggcctg ccgacttcca agagggcacg tgggtcggcg tggagctcga cctgccctca
     4021 ggtaagaatg acggttccat cggcgggaag cagtacttca ggtgtaaccc tggctacggg
     4081 ctgctggtca ggcccagccg ggtccgcagg gccacgggcc ctgtgtggcg gcgcagcaca
     4141 ggactccggc tgggtgcccc cgaggcccgc cggagcgcca ccctctcggg ctccgccacc
     4201 aacctggcct cgctgacagc tgccctggcc aaggccgaca ggagccacaa gaaccctgag
     4261 aaccggaaat cctgggccag ctgaggatcc accggatcta gataactgat cataatcagc
     4321 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     4381 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     4441 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4501 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     4561 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     4621 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     4681 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4741 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     4801 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4861 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4921 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4981 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5041 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5101 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     5161 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5221 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     5281 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     5341 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5401 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     5461 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     5521 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     5581 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5641 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5701 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5761 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5821 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5881 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5941 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     6001 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     6061 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     6121 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     6181 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     6241 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     6301 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     6361 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     6421 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     6481 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     6541 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     6601 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     6661 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     6721 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     6781 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6841 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6901 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6961 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7021 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     7081 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7141 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7201 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7261 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7321 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7381 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7441 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7501 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7561 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7621 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     7681 gttccg
//
