LOCUS       sequence-178444-        8523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pEGFP-C1 with cDNA encoding HsKIF13B aa residues 561-1826.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Schou KB, Mogensen JB, Morthorst SK, Nielsen BS, Aleliunaite A, 
            Serra-Marques A, Furstenberg N, Saunier S, Bizet AA, Veland IR, 
            Akhmanova A, Christensen ST, Pedersen LB
  TITLE     KIF13B establishes a CAV1-enriched microdomain at the ciliary 
            transition zone to promote Sonic hedgehog signalling.
  JOURNAL   Nat Commun. 2017 Jan 30;8:14177. doi: 10.1038/ncomms14177.
  PUBMED    28134340
REFERENCE   2  (bases 1 to 8523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-01-30"; volume: "8:14177. doi"; pages: " 
            10.1038/ncomms14177"
COMMENT     Sequence Label: LP725
FEATURES             Location/Qualifiers
     source          1..8523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5251..5372
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5288..5307)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5342..5361
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5379..5834)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5516..5537)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5728..5747
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5861..5965
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5967..6324
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5967..6158
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      6175..6310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6359..7153
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6359..7153
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6413..6432)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7023..7042
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7341..7360)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7385..7432
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7761..8349
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8250..8269
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1021 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1321 tccatgaaga acgagaatag ttctgagcag ctggatgtag acggagactc ctccagcgag
     1381 gtgtccagtg aagttaactt taattacgaa tacgcacaga tggaggtcac catgaaggcc
     1441 ctgggcagca atgatccgat gcagtccata ttaaacagcc tagaacaaca gcatgaagaa
     1501 gaaaaacgat ctgcactgga gcgccagagg cttatgtatg agcacgaatt ggagcagctc
     1561 cggagaaggc tgtctcctga gaagcagaac tgccggagca tggacaggtt ttctttccac
     1621 tcgcccagcg ctcagcaacg cttaagacag tgggctgagg agagagaagc aacgttgaat
     1681 aacagcctga tgaggctgag ggaacaaatt gttaaggcca atctattggt gagagaagct
     1741 aattacattg ctgaggagct ggataaaaga acagaataca aagttaccct acagattcca
     1801 gcctccagcc tggatgccaa caggaagcga ggctctcttc ttagtgagcc tgcaatccag
     1861 gtgagaagaa aaggaaaagg aaagcagatt tggtctttgg aaaaactgga caacaggctg
     1921 ttggatatga gagaccttta tcaggagtgg aaagagtgtg aagaagataa cccagtaata
     1981 cgatcatact tcaaacgtgc tgatccattc tatgatgagc aggaaaatca cagtctcatt
     2041 ggggtggcca atgtcttcct cgagtcactt ttctattatg tgaagttaca atacgctgtt
     2101 cccatcatca accagaaagg agaggtggca ggtcggctgc acgtggaggt gatgcgactc
     2161 agtggtgatg ttggggagag gatcgcagga ggcgatgagg tggcagaggt ctcctttgag
     2221 aaggagaccc aggagaacaa actggtgtgc atggttaaaa tcctgcaagc tactgggttg
     2281 ccacagcatc tgtcccactt tgtgttctgc aaatacagct tctgggatca acaggagccg
     2341 gtgattgtcg ctcctgaagt ggacacctcc tcctcttccg tcagcaagga gccgcactgc
     2401 atggttgtct ttgatcattg caatgagttt tctgttaaca tcaccgaaga ctttatcgag
     2461 catctttccg aaggagcatt ggcaattgaa gtatatggac ataaaataaa cgatccccgg
     2521 aaaaaccccg ccctgtggga tttgggaatc atccaagcaa agacacgtag tcttcgggac
     2581 agatggagtg aagtgaccag gaaattggaa ttctgggttc aaatcttgga acagaatgag
     2641 aatggtgaat actgccctgt agaagtgatt tctgcaaagg atgtcccaac aggaggaatc
     2701 ttccagctcc ggcaggggca gtcccggaga gttcaagtcg aagtgaagtc agtgcaggaa
     2761 tctgggactt taccactgat ggaagaatgt atactgtctg ttggcattgg atgtgtcaaa
     2821 gttagaccgc tcagagcccc cagaacacat gagaccttcc atgaggaaga ggaagacatg
     2881 gacagctacc aggatcgaga tttagagaga cttcgtagaa aatggctaaa tgcattaaca
     2941 aaacgtcagg agtacttgga tcaacaattg caaaagcttg tcagtaaacg tgataaaaca
     3001 gaggatgatg ctgaccgcga agcgcagctt ctggagatgc ggttgaccct aactgaggag
     3061 aggaacgcgg tgatggtccc ctctgctggc agtggtattc caggggcccc agcagaatgg
     3121 accccagtac ctgggatgga gacacacatt cctgttatat tcctggactt aaatgctgat
     3181 gatttcagct ctcaggataa tcttgatgac ccagaagctg gtggatggga tgcgaccttg
     3241 actggggaag aagaagagga gttctttgaa ttgcagattg tgaagcagca tgatggggag
     3301 gtgaaagcag aagcctcctg ggactccgcg gtgcatggct gccctcagct cagcaggggc
     3361 acgcccgtgg acgagcggtt gttcctgatc gtgcgcgtga cggtccagct cagccaccct
     3421 gctgacatgc aactggtgtt acgcaagaga atctgtgtca atgttcacgg ccgccagggt
     3481 tttgcacaga gtctcctaaa aaagatgtct catcgaagtt ctattcctgg ctgtggagtg
     3541 acttttgaaa ttgtctccaa tattccagag gatgcccagg gagtggaaga acgggaagca
     3601 ttagcaagaa tggcagccaa tgttgaaaac ccagcttctg ctgactcgga ggcttatatt
     3661 gaaaagtacc tcaggagcgt gctggctgta gaaaacctcc tgactttaga tcgtctgcgc
     3721 caggaagttg cagtgaagga acagttaaca ggaaaaggaa agttgagcag gaggagtatc
     3781 agttctccaa atgtgaacag attgtctgga agccgacaag atctcattcc atcatacagt
     3841 ctaggcagca acaagggccg gtgggaaagt cagcaggatg tatcccaaac cacagtttcc
     3901 agaggaatag ctcctgcccc cgccctctct gtttctcccc aaaataacca ttctccagat
     3961 ccaggactca gtaaccttgc agcatcctac ttgaatcctg tcaaatcctt cgtgccgcaa
     4021 atgccaaagc tcctcaagtc tctctttccc gtccgcgatg agaagagggg caagcggccg
     4081 tctcccctcg cacaccagcc cgtgccccgc atcatggtgc agtcagccag cccggacatc
     4141 agggtgacca ggatggagga ggctcagccg gagatgggcc ctgacgtgct ggtgcagacg
     4201 atgggggccc cggccttgaa gatctgcgac aaacctgcca aagtgccttc cccaccgcct
     4261 gtcatagctg tcacagcggt caccccggct ccggaggcac aggacgggcc ccccagcccc
     4321 ctgagtgaag cctctagcgg gtacttctcc cacagcgtct ccaccgcgac cctgtcggac
     4381 gccctgggcc ccggcctgga cgctgcggcc ccgccggggt ccatgcccac cgcccctgag
     4441 gccgagcccg aggcgcccat cagccacccc ccaccgccca cggccgtccc cgccgaggag
     4501 ccccctggcc cccagcagct cgtgagcccc ggtcgggagc gccccgacct cgaggccccg
     4561 gcgcccggct ccccgttccg cgtccggagg gtgcgggcct cggagttgcg ctccttctcg
     4621 cgcatgctgg ctggggaccc cggctgctcc ccgggggccg aggggaatgc gccggccccg
     4681 ggcgccgggg gacaggccct ggcctctgat tccgaggaag ctgacgaggt cccggagtgg
     4741 ctccgagagg gcgagttcgt caccgtgggc gcccacaaaa cgggcgtggt gagatacgtg
     4801 gggcctgccg acttccaaga gggcacgtgg gtcggcgtgg agctcgacct gccctcaggt
     4861 aagaatgacg gttccatcgg cgggaagcag tacttcaggt gtaaccctgg ctacgggctg
     4921 ctggtcaggc ccagccgggt ccgcagggcc acgggccctg tgtggcggcg cagcacagga
     4981 ctccggctgg gtgcccccga ggcccgccgg agcgccaccc tctcgggctc cgccaccaac
     5041 ctggcctcgc tgacagctgc cctggccaag gccgacagga gccacaagaa ccctgagaac
     5101 cggaaatcct gggccagctg aggatccacc ggatctagat aactgatcat aatcagccat
     5161 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     5221 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     5281 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5341 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt
     5401 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     5461 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     5521 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     5581 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     5641 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     5701 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     5761 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     5821 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5881 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5941 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     6001 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6061 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6121 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6181 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6241 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     6301 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     6361 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     6421 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     6481 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     6541 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     6601 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     6661 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     6721 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     6781 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     6841 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     6901 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     6961 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     7021 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     7081 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     7141 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     7201 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     7261 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     7321 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     7381 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     7441 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     7501 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     7561 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     7621 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7681 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7741 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7801 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7861 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7921 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7981 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8041 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8101 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8161 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8221 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8281 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8341 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8401 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     8461 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     8521 ccg
//
