LOCUS       sequence-178447-       11993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11993)
  AUTHORS   Ledoux S, Guthrie C
  TITLE     Retinitis Pigmentosa Mutations in Bad Response to Refrigeration 2 
            (Brr2) Impair ATPase and Helicase Activity.
  JOURNAL   J Biol Chem. 2016 Jun 3;291(23):11954-65. doi: 
            10.1074/jbc.M115.710848. Epub 2016 Apr 12.
  PUBMED    27072132
REFERENCE   2  (bases 1 to 11993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.710848"; journalName: "J Biol Chem"; date: 
            "2016-06-3- 3"; volume: "291"; issue: "23"; pages: "11954-65"
COMMENT     Sequence Label: pSL7 - Brr2 del247 N1104L
FEATURES             Location/Qualifiers
     source          1..11993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(716..1378)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(1379..1566)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(1713..1732)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1832..1854
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1892..1910)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1958..2461
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        2491..2595
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2596..2664,2665..3456)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2814..2833)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3627..4215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4116..4135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4369..4386
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4503..4524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4539..4544,4545..4562,4563..4569)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4577..4593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4582..4604
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4601..4617
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4601..4617
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4636..4656
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4638..4656
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4711..5354
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5247..5270
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             11134..11211
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             11239..11259
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             11299..11472
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             11473..11646
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     primer_bind     complement(11710..11726)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11710..11726)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(11752..11771)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11753..11771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11781..11798)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11781..11797)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11790..11812)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
      481 gcatctgtgc ggtatttcac accgcataga tccgtcgagt tcaagagaaa aaaaaagaaa
      541 aagcaaaaag aaaaaaggaa agcgcgcctc gttcagaatg acacgtatag aatgatgcat
      601 taccttgtca tcttcagtat catactgttc gtatacatac ttactgacat tcataggtat
      661 acatatatac acatgtatat atatcgtatg ctgcagcttt aaataatcgg tgtcactaca
      721 taagaacacc tttggtggag ggaacatcgt tggtaccatt gggcgaggtg gcttctctta
      781 tggcaaccgc aagagccttg aacgcactct cactacggtg atgatcattc ttgcctcgca
      841 gacaatcaac gtggagggta attctgctag cctctgcaaa gctttcaaga aaatgcggga
      901 tcatctcgca agagagatct cctactttct ccctttgcaa accaagttcg acaactgcgt
      961 acggcctgtt cgaaagatct accaccgctc tggaaagtgc ctcatccaaa ggcgcaaatc
     1021 ctgatccaaa cctttttact ccacgcacgg cccctagggc ctctttaaaa gcttgaccga
     1081 gagcaatccc gcagtcttca gtggtgtgat ggtcgtctat gtgtaagtca ccaatgcact
     1141 caacgattag cgaccagccg gaatgcttgg ccagagcatg tatcatatgg tccagaaacc
     1201 ctatacctgt gtggacgtta atcacttgcg attgtgtggc ctgttctgct actgcttctg
     1261 cctctttttc tgggaagatc gagtgctcta tcgctagggg accacccttt aaagagatcg
     1321 caatctgaat cttggtttca tttgtaatac gctttactag ggctttctgc tctgtcatct
     1381 ttgccttcgt ttatcttgcc tgctcatttt ttagtatatt cttcgaagaa atcacattac
     1441 tttatataat gtataattca ttatgtgata atgccaatcg ctaagaaaaa aaaagagtca
     1501 tccgctaggt ggaaaaaaaa aaatgaaaat cattaccgag gcataaaaaa atatagagtg
     1561 tactagagga ggccaagagt aatagaaaaa gaaaattgcg ggaaaggact gtgttatgac
     1621 ttccctgact aatgccgtgt tcaaacgata cctggcagtg actcctagcg ctcaccaagc
     1681 tcttaaaacg ggaatttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     1741 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     1801 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     1861 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     1921 aatgtcatga taataatggt ttcttagggt ccttttcatc acgtgctata aaaataatta
     1981 taatttaaat tttttaatat aaatatataa attaaaaata gaaagtaaaa aaagaaatta
     2041 aagaaaaaat agtttttgtt ttccgaagat gtaaaagact ctagggggat cgccaacaaa
     2101 tactaccttt tatcttgctc ttcctgctct caggtattaa tgccgaattg tttcatcttg
     2161 tctgtgtaga agaccacaca cgaaaatcct gtgattttac attttactta tcgttaatcg
     2221 aatgtatatc tatttaatct gcttttcttg tctaataaat atatatgtaa agtacgcttt
     2281 ttgttgaaat tttttaaacc tttgtttatt tttttttctt cattccgtaa ctcttctacc
     2341 ttctttattt actttctaaa atccaaatac aaaacataaa aataaataaa cacagagtaa
     2401 attcccaaat tattccatca ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat
     2461 ccgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2521 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2581 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     2641 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     2701 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     2761 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     2821 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     2881 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     2941 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3001 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3061 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3121 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     3181 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3241 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3301 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3361 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3421 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     3481 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3541 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3601 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3661 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3721 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3781 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3841 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3901 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3961 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4021 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4081 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4141 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4201 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4261 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4321 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4381 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4441 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4501 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     4561 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     4621 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccaccgc
     4681 ggtggcggcc gcggctgcag gtcgagttta tcattatcaa tactgccatt tcaaagaata
     4741 cgtaaataat taatagtagt gattttccta actttattta gtcaaaaaat tagcctttta
     4801 attctgctgt aacccgtaca tgcccaaaat agggggcggg ttacacagaa tatataacat
     4861 cgtaggtgtc tgggtgaaca gtttattcct ggcatccact aaatataatg gagcccgctt
     4921 tttaagctgg catccagaaa aaaaaagaat cccagcacca aaatattgtt ttcttcacca
     4981 accatcagtt cataggtcca ttctcttagc gcaactacag agaacagggg cacaaacagg
     5041 caaaaaacgg gcacaacctc aatggagtga tgcaacctgc ctggagtaaa tgatgacaca
     5101 aggcaattga cccacgcatg tatctatctc attttcttac accttctatt accttctgct
     5161 ctctctgatt cggaaaaagc tgaaaaaaaa ggttgaaacc agttccctga aattactccc
     5221 ctacttgact aataagtata taaagacggt aggtattgat tgtaattctg taaatctatt
     5281 tcttaaactt cttaaattct acttttatag ttagtctttt ttttagtttt aaaacaccaa
     5341 gaacttagtt tcgactctag aatgtacaat gacgttgaag tcaattctaa aaaaaaaaac
     5401 aagagggcat tgccaaacat tgaaaacgat ataattaaac tctccgatag taaaacaagt
     5461 aatatcgaga gcgttccgat atactcaatc gatgaatttt ttttgcagag aaaacttcgg
     5521 tcagagcttg gttacaagga tacctctgta atacaagacc tgtcagaaaa aattttgaat
     5581 gatattgaaa cacttgagca taatccagta gcgttggagc agaagttggt tgatctgctt
     5641 aaattcgaga atatatcact ggctgaattt attctcaaga atagatctac aattttttgg
     5701 ggcatacgac tggcgaagag cacagagaat gaaataccga acttaataga gaaaatggtt
     5761 gcaaaaggtt tgaatgatct tgtggaacag tataaattta gagaaactac acactcaaag
     5821 cgtgaactcg attccggtga cgaccagcca cagtcttcgg aagcaaaaag aacaaaattc
     5881 agtaatccag caataccgcc cgtcatagat ttggaaaaga ttaaattcga cgaaagttct
     5941 aaattaatga ccgtgacaaa agtatcccta ccggaaggat cattcaaaag ggtgaaacca
     6001 caatatgacg aaatacatat tccagctcct agcaaacctg ttattgatta tgagttgaaa
     6061 gaaattactt ctctaccaga ttggtgtcag gaggcgtttc cttcatcgga aacaacgtcc
     6121 ttaaacccta tacaatcaaa ggtttttcac gcagcatttg aaggtgattc aaacatgcta
     6181 atatgtgcac ccactggttc aggtaagacg aatatcgcgt tattgacggt attgaaagcc
     6241 ttatcccatc attataaccc gaaaactaag aaattaaacc tttctgcatt taaaattgtt
     6301 tatatagcac ccttgaaagc cttagtacag gaacaagtcc gagaatttca aaggaggttg
     6361 gcttttctag gtataaaagt cgctgaattg actggtgatt ctaggcttag tagaaagcaa
     6421 attgacgaga cgcaggtttt ggtgtctacg ccagagaaat gggacataac tactagaaat
     6481 tcaaataatt tggccatcgt agaacttgtt cgtttgttga ttatagatga aatacatttg
     6541 ttacatgatg acaggggacc agtattggaa agcatcgtgg ctaggacatt ttgggcttcc
     6601 aaatatggcc aggaataccc cagaataata ggcctttctg ccaccttacc aaattatgag
     6661 gatgtcggta ggtttttaag agttccgaag gaaggattgt tttattttga ctcctcattt
     6721 agaccgtgtc ctctatctca acagttttgt ggcattaagg aacggaactc cttgaaaaag
     6781 ctgaaggcta tgaatgatgc ttgttatgaa aaggtattgg aatccatcaa tgaaggtaat
     6841 caaataatcg tgtttgttca ttctcgaaaa gaaacatcac gaacagcaac atggttgaaa
     6901 aataaatttg ctgaagagaa cattactcat aaattaacca aaaatgatgc aggatcgaag
     6961 cagattttaa agacagaagc agccaacgta ctcgatccaa gtctacgaaa gttgattgaa
     7021 agcggtatag ggacgcatca cgcagggctg accagaagtg acagatcctt atctgaagat
     7081 ttatttgcag acgggttgtt gcaagtattg gtatgcactg cgacgttagc atggggtgtt
     7141 aatctacctg ctcatacagt gattataaaa ggaacagatg tctattcacc tgaaaagggg
     7201 tcctgggaac aactttctcc acaagatgta cttcaaatgt taggtagagc aggcagaccg
     7261 agatatgaca catttggcga gggtattata ataacagacc aatcgaatgt tcaatattat
     7321 ctctcggttt taaatcaaca attaccaata gaatcgcaat ttgtttcaaa attagttgac
     7381 aacttaaatg cagaagttgt cgcgggtaac attaagtgca ggaatgatgc tgtgaattgg
     7441 ttggcgtata cttacctata tgtcagaatg ttagcttccc ctatgcttta caaagtacct
     7501 gatatttcaa gtgatgggca attgaaaaag tttagagaaa gcttagtcca ttcggcccta
     7561 tgcattttaa aagaacaaga gttggtgtta tatgatgctg aaaatgatgt aattgaggcg
     7621 accgatctag gaaatatagc gtcgtcattt tacataaatc atgcttccat ggatgtttac
     7681 aatagagagt tagatgaaca tacaactcag attgatcttt ttagaatatt ctcaatgtcg
     7741 gaagagttta agtacgtatc ggtgagatat gaagagaaaa gagaactaaa acaacttcta
     7801 gagaaagcac ccattcctat cagagaagat attgatgatc ctttagcaaa agtgaatgta
     7861 ctactgcagt cttatttttc ccagctaaag ttcgaaggtt ttgcacttaa ttctgatata
     7921 gtgtttattc accaattggc tggtaggtta ttgcgtgcta tgtttgaaat atgcttgaag
     7981 agaggatggg gacatcccac acggatgttg ttaaatcttt gcaaatctgc cacaactaaa
     8041 atgtggccaa caaactgtcc tttaagacag tttaaaacat gccctgtcga agtgattaaa
     8101 aggctggaag cctctacagt accgtgggga gattatctac agctagaaac acctgccgaa
     8161 gttggtcgtg caatcagatc tgaaaaatat ggaaaacagg tttacgattt attgaaaaga
     8221 tttcccaaaa tgtccgtaac atgcaacgct cagccaatta cccgtagcgt tatgaggttc
     8281 aacattgaaa taatagccga ctggatatgg gatatgaatg tacacggttc ccttgaacct
     8341 tttttgctca tgctagaaga cactgatggt gattcaatac tttattatga cgtactattc
     8401 atcactccag atatagttgg tcatgaattt accttatcct ttacttatga gttaaagcag
     8461 cacaatcaaa ataacttacc acctaatttc ttccttactt tgatatcaga aaattggtgg
     8521 cacagtgaat tcgagattcc agtttccttc aatggattta aacttccgaa aaaatttcct
     8581 cctcctactc cccttctgga gaatattagt atttcaacat cagagcttgg caatgatgat
     8641 ttttccgaag tatttgaatt taaaactttc aacaagattc agagccaggt cttcgagtct
     8701 ctctacaatt cgaatgactc ggtctttgta ggttcaggaa agggcacagg taaaacagct
     8761 atggctgaat tagcactgtt aaatcattgg agacagaata aaggaagagc ggtttatatc
     8821 aatccgtctg gggaaaaaat tgattttttg ctatctgatt ggaataaaag gttctctcat
     8881 cttgctggtg gtaagataat aaataagcta ggaaatgatc cttcactaaa tttaaaatta
     8941 ttagcaaaaa gccatgttct cttggctacc cccgtgcaat ttgagctttt gtcacgtcgt
     9001 tggagacaaa ggaagaacat ccaaagttta gaattaatga tatatgatga cgctcatgag
     9061 attagccaag gtgtttacgg tgctgtgtat gaaacgctaa tttctagaat gatatttata
     9121 gcaacccaac ttgaaaagaa aattcgattt gtttgccttt cgaactgttt ggcaaatgct
     9181 cgtgattttg gtgaatgggc tggtatgacg aagtcaaaca tctacaactt ttctccaagc
     9241 gaaagaattg agcctcttga aattaatatt cagtcattta aagatgttga gcacatatca
     9301 ttcaattttt cgatgcttca aatggcattt gaagcatctg ctgctgcagc gggaaatcgt
     9361 aattccagtt cggtgttctt accatctaga aaggattgta tggaagttgc gtctgcattt
     9421 atgaagtttt caaaagcaat tgaatgggat atgttgaacg ttgaagaaga gcaaattgta
     9481 ccatacattg aaaagttaac agatggtcat ctaagagcac ctttaaaaca cggtgttggt
     9541 attttgtata aggggatggc atcaaatgat gaaagaattg tgaaaaggtt atacgaatat
     9601 ggtgcggtat cggttttact catttctaag gattgttctg cattcgcttg taaaactgat
     9661 gaagtgatta ttttaggaac caacctttat gatggtgcag agcataaata tatgccatat
     9721 accataaatg aacttctaga aatggtggga ctagctagtg gtaacgattc aatggcaggc
     9781 aaggttttaa ttttgacaag tcacaacatg aaggcctact acaaaaaatt cttaattgag
     9841 cctttgccta ctgagagtta cctacagtat attattcatg atactctgaa caacgagata
     9901 gcaaattcta ttatacaaag caaacaagat tgtgttgact ggtttacata ttcgtatttc
     9961 tatcgtcgta tccatgtgaa tccaagctat tatggcgtaa gggatacgtc tccacatgga
    10021 atttcagttt ttttgagtaa tttagttgaa acttgtttga atgacttagt tgaatcatcc
    10081 ttcattgaaa ttgacgatac agaagctgaa gtaaccgcag aagttaatgg aggagatgat
    10141 gaagcaactg aaatcatttc taccttgagc aacgggctaa ttgcttctca ctatggcgta
    10201 tcatttttta ctattcagtc attcgtttct tctttatcga atacgtcaac gctcaaaaac
    10261 atgctgtatg ttctttcaac agcagtagaa ttcgaaagtg ttccactcag aaaaggagac
    10321 agagcattac ttgtcaaatt aagtaaaaga ctaccattga gattccctga acacacctct
    10381 tctggttcgg tgagctttaa agttttctta ttgttacaag catatttttc acgtcttgaa
    10441 ttacctgtcg actttcaaaa tgatttaaaa gacattctag aaaaggttgt tccactaatt
    10501 aacgtagtgg ttgatatcct ttcagcaaac gggtatttga atgctactac cgccatggat
    10561 ttggcgcaga tgttgattca gggtgtatgg gacgttgata atccgttgag acagattcca
    10621 cacttcaaca acaaaatttt ggaaaaatgt aaagaaatta acgtcgagac ggtctatgat
    10681 ataatggccc ttgaggatga agagagggat gaaattttaa cgcttactga ttcacagctt
    10741 gcgcaggtcg ccgcgtttgt taacaattac cccaacgttg aacttactta ttcgttgaat
    10801 aattctgatt cgttaatttc aggtgtaaaa cagaaaatta ccattcaatt gacaagagat
    10861 gttgaacctg agaacttaca agtaacatct gaaaagtatc cgtttgataa gctagaaagt
    10921 tggtggttgg ttttaggtga agtatctaaa aaagagcttt atgcgatcaa gaaagttact
    10981 ttaaataaag agactcagca atatgaattg gaatttgaca ctccgacatc tggtaaacat
    11041 aacttaacaa tttggtgtgt ctgtgattca tatcttgacg cagataaaga gttgtccttt
    11101 gaaataaatg tgaaactcga gggatccatg gaaaagagaa gatggaaaaa gaatttcata
    11161 gccgtctcag cagccaaccg ctttaagaaa atctcatcct ccggggcact tgattatgat
    11221 attccaacta ctgctagcga gaatttgtat tttcagggtg agctcaaaac cgcggctctt
    11281 gcgcaacacg atgaagccgt ggacaacaaa ttcaacaaag aacaacaaaa cgcgttctat
    11341 gagatcttac atttacctaa cttaaacgaa gaacaacgaa acgccttcat ccaaagttta
    11401 aaagatgacc caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatgatgct
    11461 caggcgccga aagtagacaa caaattcaac aaagagcaac aaaacgcgtt ctatgagatc
    11521 ttacatttac ctaacttaaa cgaagaacaa cgaaacgcct tcatccaaag tttaaaagat
    11581 gacccaagcc aaagcgctaa ccttttagca gaagctaaaa agctaaatgg tgctcaggcg
    11641 ccgaaagtag acgcgaattc cgcggggaag tcaacctgaa gcttgatatc gaattcctgc
    11701 agcccggggg atccactagt tctagagcgg ccgggcccgg tacccaattc gccctatagt
    11761 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    11821 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    11881 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
    11941 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggg ttacgcgcag cgg
//
