LOCUS       sequence-178453-        5182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mycobacterial expression of mCherry from Hsp60 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Oldfield LM, Hatfull GF
  TITLE     Mutational analysis of the mycobacteriophage BPs promoter PR reveals
            context-dependent sequences for mycobacterial gene expression.
  JOURNAL   J Bacteriol. 2014 Oct;196(20):3589-97. doi: 10.1128/JB.01801-14. 
            Epub 2014 Aug 4.
  PUBMED    25092027
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.01801-14"; journalName: "J Bacteriol"; date: "2014-10"; 
            volume: "196"; issue: "20"; pages: "3589-97"
COMMENT     Sequence Label: pLO87
FEATURES             Location/Qualifiers
     source          1..5182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2315..2361
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2511..2530)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2522..3337
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2595..2614)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3668..4256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4157..4176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctctac acactcagcc acatcgaggc cgagctcggc gcgaacatgc caccgccgcg
       61 ctggcgtcag cagaccacgt acaaagcggc tccgacgccg ctagggcgga attgcgcact
      121 gttcgattcc gtcaggttgt gggcctatcg tcccgccctc atgcggatct acctgccgac
      181 ccggaacgtg gacggactcg gccgcgcgat ctatgccgag tgccacgcgc gaaacgccga
      241 atttccgtgc aacgacgtgt gtcccggacc gctaccggac agcgaggtcc gcgccatcgc
      301 caacagcatt tggcgttgga tcacaaccaa gtcgcgcatt tgggcggacg ggatcgtggt
      361 ctacgaggcc acactcagtg cgcgccagtc ggccatctcg cggaagggcg cagcagcgcg
      421 cacggcggcg agcacagttg cgcggcgcgc aaagtccgcg tcagccatgg aggcattgct
      481 atgagcgacg gctacagcga cggctacaac cggcagccga ctgtccgcaa aaagcggcgc
      541 gtgaccgccg ccgaaggcgc tcgaatcacc ggactatccg aacgccacgt cgtccggctc
      601 gtggcgcagg aacgcagcga gtggctcgcc gagcaggctg cacgccgcga acgcatccgc
      661 gcctatcacg acgacgaggg ccactcttgg ccgcaaacgg ccaaacattt cgggctgcat
      721 ctggacaccg ttaagcgact cggctatcgg gcgaggaaag agcgtgcggc agaacaggaa
      781 gcggctcaaa aggcccacaa cgaagccgac aatccaccgc tgttctaacg caattgggga
      841 gcgggtgtcg cgggggttcc gtggggggtt ccgttgcaac gggtcggaca ggtaaaagtc
      901 ctggtagacg ctagttttct ggtttgggcc atgcctgtct cgttgcgtgt ttcgttgcgc
      961 ccgttttgaa taccagccag acgagacggg gttctacgaa tcttggtcga taccaagcca
     1021 tttccgctga atatcgggga gctcaccgcc agaatcggtg gttgtggtga tgtacgtggc
     1081 gaactccgtt gtagtgcctg tggtggcatc cgtggcgcgg ccgcggtacc agatctttaa
     1141 atctagacgg tgaccacaac gcgcccgctt tgatcgggga cgtctgcggc cgaccattta
     1201 cgggtcttgt tgtcgttggc ggtcatgggc cgaacatact cacccggatc ggagggccga
     1261 ggacaaggtc gaacgagggg catgacccgg tgcggggctt cttgcactcg gcataggcga
     1321 gtgctaagaa taacgttggc actcgcgacc ggtgagtgct aggtcgggac ggtgaggcca
     1381 ggcccgtcgt cgcagcgagt ggcagcgagg acaacttgag ccgtccgtcg cgggcactgc
     1441 gcccggccag cgtaagtagc ggggttgccg tcacccggtg acccccgttt catccccgat
     1501 ccggacggcg ggagaaagga gaaagccaat catggtctcg aagggcgagg aggacaacat
     1561 ggccatcatc aaagagttca tgcgcttcaa ggtgcacatg gagggcagcg tcaacggcca
     1621 cgagttcgag atcgagggcg agggcgaggg ccggccgtac gagggcacgc agaccgcgaa
     1681 gctgaaggtg acgaagggcg gccccctgcc gttcgcctgg gacatcctgt ccccccagtt
     1741 catgtacggc tcgaaggcgt acgtcaagca cccggccgac atccccgact acctgaagct
     1801 gagcttcccg gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtcgt
     1861 gaccgtcacg caggactcct cgctgcagga cggcgagttc atctacaagg tgaagctgcg
     1921 gggcaccaac ttccccagcg acggcccggt catgcagaag aagacgatgg gctgggaggc
     1981 gtcctcggag cgcatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagcggct
     2041 gaagctgaag gacggcggcc actacgacgc ggaggtgaag accacgtaca aggccaagaa
     2101 gccggtccag ctgcccggcg cgtacaacgt gaacatcaag ctggacatca ccagccacaa
     2161 cgaggactac accatcgtcg agcagtacga gcgcgccgag ggccggcact cgaccggcgg
     2221 catggacgag ctgtacaagt aaggatccag ctgcagaatt cgaagcttat cgatgtcgac
     2281 gtagttaact agcgtacgat cgactgccag gcatcaaata aaacgaaagg ctcagtcgaa
     2341 agactgggcc tttcgtttta tgccatcatg gccgcggtga tcagctagcc acctgacgtc
     2401 gggggggggg gaaagccacg ttgtgtctca aaatctctga tgttacattg cacaagataa
     2461 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     2521 tatgagccat attcaacggg aaacgtcttg ctcgaggccg cgattaaatt ccaacatgga
     2581 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     2641 ctatcgcttg tatgggaagc cccatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     2701 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     2761 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     2821 gatccccggg aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     2881 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     2941 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     3001 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     3061 agaaatgcat aatcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     3121 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     3181 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     3241 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     3301 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     3361 acactggcag agcattacgc tgacttgacg ggacggcggc tttgttgaat aaatcgaact
     3421 tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca
     3481 aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcac caaccgtggc
     3541 tccctcactt tctggctgga tgatggggcg attcaggcct ggtatgagtc agcaacacct
     3601 tcttcacgag gcagacctca ctagttccat gagcgtcaga ccccgtagaa aagatcaaag
     3661 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3721 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3781 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3841 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3901 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3961 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4021 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4081 ccgaggggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4141 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4201 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4261 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4321 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4381 ccgctcgccg cagccgaacg accgagcgca acgcgtgagc ccaccagctc cgtaagttcg
     4441 ggcgctgtgt ggctcgtacc cgcgcattca ggcggcaggg ggtctaacgg gtctaaggcg
     4501 gcgtgtacgg ccgccacagc ggctctcagc ggcccggaaa cgtcctcgaa acgacgcatg
     4561 tgttcctcct ggttggtaca ggtggttggg ggtgctcggc tgtcgctggt gttccaccac
     4621 cagggctcga cgggagagcg ggggagtgtg cagttgtggg gtggcccctc agcgaaatat
     4681 ctgacttgga gctcgtgtcg gaccatacac cggtgattaa tcgtggtcta ctaccaagcg
     4741 tgagccacgt cgccgacgaa tttgagcagc tctggctgcc gtactggccg ctggcaagcg
     4801 acgatctgct cgaggggatc taccgccaaa gccgcgcgtc ggccctaggc cgccggtaca
     4861 tcgaggcgaa cccaacagcg ctggcaaacc tgctggtcgt ggacgtagac catccagacg
     4921 cagcgctccg agcgctcagc gcccgggggt cccatccgct gcccaacgcg atcgtgggca
     4981 atcgcgccaa cggccacgca cacgcagtgt gggcactcaa cgcccctgtt ccacgcaccg
     5041 aatacgcgcg gcgtaagccg ctcgcataca tggcggcgtg cgccgaaggc cttcggcgcg
     5101 ccgtcgatgg cgaccgcagt tactcaggcc tcatgaccaa aaaccccggc cacatcgcct
     5161 gggaaacgga atggctccac tc
//
