LOCUS       sequence-178476-        4828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mycobacterial expression of mCherry reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Oldfield LM, Hatfull GF
  TITLE     Mutational analysis of the mycobacteriophage BPs promoter PR reveals
            context-dependent sequences for mycobacterial gene expression.
  JOURNAL   J Bacteriol. 2014 Oct;196(20):3589-97. doi: 10.1128/JB.01801-14. 
            Epub 2014 Aug 4.
  PUBMED    25092027
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.01801-14"; journalName: "J Bacteriol"; date: "2014-10"; 
            volume: "196"; issue: "20"; pages: "3589-97"
COMMENT     Sequence Label: pLO86
FEATURES             Location/Qualifiers
     source          1..4828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1961..2007
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2157..2176)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2168..2983
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2241..2260)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3314..3902
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3803..3822
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctctac acactcagcc acatcgaggc cgagctcggc gcgaacatgc caccgccgcg
       61 ctggcgtcag cagaccacgt acaaagcggc tccgacgccg ctagggcgga attgcgcact
      121 gttcgattcc gtcaggttgt gggcctatcg tcccgccctc atgcggatct acctgccgac
      181 ccggaacgtg gacggactcg gccgcgcgat ctatgccgag tgccacgcgc gaaacgccga
      241 atttccgtgc aacgacgtgt gtcccggacc gctaccggac agcgaggtcc gcgccatcgc
      301 caacagcatt tggcgttgga tcacaaccaa gtcgcgcatt tgggcggacg ggatcgtggt
      361 ctacgaggcc acactcagtg cgcgccagtc ggccatctcg cggaagggcg cagcagcgcg
      421 cacggcggcg agcacagttg cgcggcgcgc aaagtccgcg tcagccatgg aggcattgct
      481 atgagcgacg gctacagcga cggctacaac cggcagccga ctgtccgcaa aaagcggcgc
      541 gtgaccgccg ccgaaggcgc tcgaatcacc ggactatccg aacgccacgt cgtccggctc
      601 gtggcgcagg aacgcagcga gtggctcgcc gagcaggctg cacgccgcga acgcatccgc
      661 gcctatcacg acgacgaggg ccactcttgg ccgcaaacgg ccaaacattt cgggctgcat
      721 ctggacaccg ttaagcgact cggctatcgg gcgaggaaag agcgtgcggc agaacaggaa
      781 gcggctcaaa aggcccacaa cgaagccgac aatccaccgc tgttctaacg caattgggga
      841 gcgggtgtcg cgggggttcc gtggggggtt ccgttgcaac gggtcggaca ggtaaaagtc
      901 ctggtagacg ctagttttct ggtttgggcc atgcctgtct cgttgcgtgt ttcgttgcgc
      961 ccgttttgaa taccagccag acgagacggg gttctacgaa tcttggtcga taccaagcca
     1021 tttccgctga atatcgggga gctcaccgcc agaatcggtg gttgtggtga tgtacgtggc
     1081 gaactccgtt gtagtgcctg tggtggcatc cgtggcgcgg ccgcggtacc agatctttaa
     1141 atctagccgg acggcgggag aaaggagaaa gccaatcatg gtctcgaagg gcgaggagga
     1201 caacatggcc atcatcaaag agttcatgcg cttcaaggtg cacatggagg gcagcgtcaa
     1261 cggccacgag ttcgagatcg agggcgaggg cgagggccgg ccgtacgagg gcacgcagac
     1321 cgcgaagctg aaggtgacga agggcggccc cctgccgttc gcctgggaca tcctgtcccc
     1381 ccagttcatg tacggctcga aggcgtacgt caagcacccg gccgacatcc ccgactacct
     1441 gaagctgagc ttcccggagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     1501 cgtcgtgacc gtcacgcagg actcctcgct gcaggacggc gagttcatct acaaggtgaa
     1561 gctgcggggc accaacttcc ccagcgacgg cccggtcatg cagaagaaga cgatgggctg
     1621 ggaggcgtcc tcggagcgca tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     1681 gcggctgaag ctgaaggacg gcggccacta cgacgcggag gtgaagacca cgtacaaggc
     1741 caagaagccg gtccagctgc ccggcgcgta caacgtgaac atcaagctgg acatcaccag
     1801 ccacaacgag gactacacca tcgtcgagca gtacgagcgc gccgagggcc ggcactcgac
     1861 cggcggcatg gacgagctgt acaagtaagg atccagctgc agaattcgaa gcttatcgat
     1921 gtcgacgtag ttaactagcg tacgatcgac tgccaggcat caaataaaac gaaaggctca
     1981 gtcgaaagac tgggcctttc gttttatgcc atcatggccg cggtgatcag ctagccacct
     2041 gacgtcgggg gggggggaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca
     2101 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     2161 gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat taaattccaa
     2221 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     2281 gacaatctat cgcttgtatg ggaagcccca tgcgccagag ttgtttctga aacatggcaa
     2341 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     2401 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     2461 cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     2521 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     2581 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     2641 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     2701 ctggaaagaa atgcataatc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     2761 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     2821 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     2881 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     2941 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3001 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcggctttg ttgaataaat
     3061 cgaacttttg ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc agaccgttcc
     3121 gtggcaaagc aaaagttcaa aatcaccaac tggtccacct acaacaaagc tctcaccaac
     3181 cgtggctccc tcactttctg gctggatgat ggggcgattc aggcctggta tgagtcagca
     3241 acaccttctt cacgaggcag acctcactag ttccatgagc gtcagacccc gtagaaaaga
     3301 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3361 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3421 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     3481 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3541 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3601 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3661 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3721 cgcttcccga ggggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3781 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3841 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3901 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3961 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4021 ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc gtgagcccac cagctccgta
     4081 agttcgggcg ctgtgtggct cgtacccgcg cattcaggcg gcagggggtc taacgggtct
     4141 aaggcggcgt gtacggccgc cacagcggct ctcagcggcc cggaaacgtc ctcgaaacga
     4201 cgcatgtgtt cctcctggtt ggtacaggtg gttgggggtg ctcggctgtc gctggtgttc
     4261 caccaccagg gctcgacggg agagcggggg agtgtgcagt tgtggggtgg cccctcagcg
     4321 aaatatctga cttggagctc gtgtcggacc atacaccggt gattaatcgt ggtctactac
     4381 caagcgtgag ccacgtcgcc gacgaatttg agcagctctg gctgccgtac tggccgctgg
     4441 caagcgacga tctgctcgag gggatctacc gccaaagccg cgcgtcggcc ctaggccgcc
     4501 ggtacatcga ggcgaaccca acagcgctgg caaacctgct ggtcgtggac gtagaccatc
     4561 cagacgcagc gctccgagcg ctcagcgccc gggggtccca tccgctgccc aacgcgatcg
     4621 tgggcaatcg cgccaacggc cacgcacacg cagtgtgggc actcaacgcc cctgttccac
     4681 gcaccgaata cgcgcggcgt aagccgctcg catacatggc ggcgtgcgcc gaaggccttc
     4741 ggcgcgccgt cgatggcgac cgcagttact caggcctcat gaccaaaaac cccggccaca
     4801 tcgcctggga aacggaatgg ctccactc
//
