LOCUS       sequence-178479-       14645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14645)
  AUTHORS   Mayerle M, Raghavan M, Ledoux S, Price A, Stepankiw N, 
            Hadjivassiliou H, Moehle EA, Mendoza SD, Pleiss JA, Guthrie C, 
            Abelson J
  TITLE     Structural toggle in the RNaseH domain of Prp8 helps balance 
            splicing fidelity and catalytic efficiency.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Apr 17. pii: 201701462. doi: 
            10.1073/pnas.1701462114.
  PUBMED    28416677
REFERENCE   2  (bases 1 to 14645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Apr 17. pii: 201701462. doi: 
            10.1073/pnas.1701462114."
COMMENT     Sequence Label: pRS313-PRP8
FEATURES             Location/Qualifiers
     source          1..14645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9063..9082)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9064..9082)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9089..9106)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9089..9105)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9098..9120)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9246..9701
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9333..9352)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9543..9564
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9961..10623)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(10624..10811)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(10958..10977)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11077..11099
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11137..11155)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    11203..11706
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        11736..11840
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11841..11909,11910..12701)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12059..12078)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12872..13460
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13361..13380
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13614..13631
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13748..13769
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13784..13789,13790..13807,13808..13814)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13822..13838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13827..13849
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13846..13862
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13846..13862
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        13883..13901
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 atgagtggac taccgccccc acctcctggt tttgaagagg acagcgactt agcacttcca
       61 ccaccaccac caccaccgcc tggatacgaa atcgaagaac tggataatcc gatggtgcca
      121 tcatcggtaa atgaggatac attccttccg cctccaccac ctcctccaag caacttcgaa
      181 ataaacgctg aagaaattgt ggacttcaca ttaccaccgc caccaccccc tccaggtcta
      241 gatgaattag agactaaagc agaaaagaaa gtagaattac atggaaagag aaaattggac
      301 ataggaaaag atactttcgt cactcgaaaa tcaagaaagc gtgcaaaaaa aatgaccaaa
      361 aaggcaaaga gaagcaattt atatactcct aaagccgaga tgccaccgga acatctgcga
      421 aagattatta atactcacag tgacatggcc tccaaaatgt acaatactga taaaaaggct
      481 tttctaggcg ctctaaagta tttaccgcac gcgattttaa aattgttaga gaacatgcct
      541 catccatggg agcaagccaa agaagttaaa gttttatatc acacttcagg agccataaca
      601 tttgtgaatg aaacaccaag agtaatagag cctgtatata ctgcccaatg gtcagctact
      661 tggatagcaa tgaggagaga aaaacgagat agaacacatt ttaaaagaat gagatttcca
      721 cctttcgatg atgacgaacc acctctgtca tatgaacagc acatagaaaa tatcgagcca
      781 ctggatccta taaatcttcc cttagactct caagatgatg aatatgttaa agattggctc
      841 tacgattccc ggcccttgga ggaggatagt aaaaaggtca atggtacatc ttataaaaag
      901 tggagttttg atttacctga aatgtcgaac ttatataggt tatcaacgcc acttagagat
      961 gaagtaactg acaaaaatta ctattatctt ttcgataaaa aatctttttt taacggtaaa
     1021 gcccttaata acgcaatccc tggaggtcct aaatttgaac ctttataccc aagagaagag
     1081 gaggaagatt ataacgaatt caactctata gatagagtaa tatttagggt tcctattaga
     1141 agcgaatata aggtagcgtt tcctcatcta tataattccc ggccccgttc agtgcgcata
     1201 ccatggtata ataatccagt gtcgtgtatt atccagaacg atgaggagta cgacacgcct
     1261 gcactttttt ttgatccatc gctgaaccca atacctcact ttattgataa taattcatcc
     1321 ctcaacgtat caaatacaaa ggaaaacggt gatttcactt tacctgaaga ttttgcacct
     1381 cttttggctg aagaagaaga acttattttg ccaaatacaa aagatgcgat gtcgttatat
     1441 cattcacctt ttccatttaa tagaactaaa ggaaaaatgg tgagagcaca agatgttgcc
     1501 ctggctaaaa aatggttttt gcaacatcct gacgaggagt atcccgttaa agttaaggtc
     1561 tcttaccaaa agctcttgaa gaactacgtg ctaaatgaat tgcaccctac tttgccaaca
     1621 aaccacaaca aaactaagtt attgaagagt ttaaaaaata ccaagtattt tcaacaaact
     1681 acaattgatt gggtagaggc aggactccag ctatgtcgac aaggacacaa tatgttaaat
     1741 ttattaatcc acagaaaggg cctaacttat ttacatctcg actacaattt caatttaaaa
     1801 ccaactaaaa ccttaaccac aaaagaacgt aaaaagtcaa gacttggaaa ctcatttcat
     1861 ttgatgcgtg aattgctaaa aatgatgaaa ctgattgtag atacacatgt gcagtttagg
     1921 ttaggaaatg tagatgcctt ccagttagca gatggtatac attatatttt gaaccacata
     1981 ggacaattga ccggtattta tcgttataaa tataaagtaa tgcatcaaat ccgcgcatgt
     2041 aaagatctga aacatatcat atattataag tttaataaaa atttgggaaa aggacctggt
     2101 tgtggatttt ggcaacctgc gtggagagtg tggctaaatt tcttaagagg cactatacca
     2161 ctactcgaaa gatatattgg aaaccttatc acacgtcaat ttgaaggccg ttctaatgaa
     2221 attgtcaaaa ctacaactaa acaaagactt gacgcctact acgatcttga gttacgaaac
     2281 tcggtcatgg atgacatttt agaaatgatg cctgagagta ttcgacagaa aaaggcaaga
     2341 actatactgc aacatttaag tgaggcttgg agatgttgga aggctaatat tccatgggat
     2401 gttcctggta tgccagcccc aatcaaaaaa ataattgaac ggtatataaa atccaaagct
     2461 gatgcctggg tatctgcagc tcattacaat cgagaacgta taaagcgtgg agctcacgtc
     2521 gaaaagacta tggttaaaaa aaatctgggg cgtttaacga gactttggat caaaaatgag
     2581 caggaacgtc aaaggcaaat acagaaaaac ggtcccgaaa ttactcccga agaggctacg
     2641 accatatttt ctgttatggt tgagtggctt gaatcaagga gcttttcacc aataccattt
     2701 ccacctttaa cttacaaaaa tgatactaag atattagttc ttgccctcga agacctaaaa
     2761 gatgtctacg catcaaaagt acgtttaaat gcatctgaaa gggaagaact tgcgttgata
     2821 gaggaggctt atgataatcc tcacgatact ttgaacagaa tcaaaaagta cttgttgacc
     2881 cagcgagttt ttaagcctgt tgatataacc atgatggaaa actatcaaaa catttcccct
     2941 gtttattcag ttgatccctt ggaaaagatt accgatgcat atcttgatca gtatttatgg
     3001 tacgaagctg accaacgaaa gcttttccca aactggatta aaccaagtga ttcagagata
     3061 ccacctcttc tggtatacaa gtggactcag ggtataaaca acctatctga aatttgggat
     3121 gtatccagag gccaatctgc agttttgctt gagactactt tgggtgaaat ggccgaaaaa
     3181 attgacttta ctttacttaa tagattactt cgcctgattg tagatcctaa tattgctgac
     3241 tatatcactg caaaaaataa tgttgttatc aactttaaag atatgagtca cgtcaacaaa
     3301 tatggcttaa tacgcgggtt gaagttcgct tctttcatat tccaatatta cggactagtt
     3361 atagatcttt tactattggg tcaggaaagg gctacagatt tggctggtcc agctaacaat
     3421 ccaaatgaat ttatgcaatt caagagcaaa gaagtagaaa aggcacatcc gatcagactt
     3481 tacaccagat atttagatcg tatatatatg ctttttcact ttgaagaaga tgagggagag
     3541 gaattgactg atgaatactt ggcagagaat ccagatccaa actttgaaaa cagcatcgga
     3601 tacaataata gaaaatgctg gcccaaagat tcgcgcatga ggctaatacg tcaggacgtt
     3661 aacctagggc gagcagtgtt ttgggaaatt caaagtaggg tgccaacttc gttaacgtca
     3721 attaaatggg aaaatgcttt tgtttccgta tacagtaaaa acaatccgaa cttactcttc
     3781 tctatgtgtg gttttgaagt cagaatatta ccaagacaaa gaatggaaga ggtggtttct
     3841 aacgatgaag gtgtttggga tttagttgac gaaagaacca agcaaagaac tgcaaaagca
     3901 tatttaaagg tatctgaaga agaaatcaaa aaatttgaca gcagaataag aggcatattg
     3961 atggcctccg gttcaaccac ttttactaaa gtggccgcta aatggaacac ttcactgata
     4021 tcccttttca catactttag agaagccatt gttgccacgg agccgttatt agatatcctg
     4081 gtgaaaggag aaacaagaat tcaaaaccgt gtcaaacttg gtttaaattc gaaaatgcca
     4141 acaagatttc cacctgctgt tttttataca ccaaaagaac ttggtgggtt gggtatgatt
     4201 agtgcttctc atattttaat tcctgcatct gacttaagtt ggtcaaaaca gaccgatact
     4261 ggaattacgc attttcgtgc tggtatgacg cacgaggatg aaaaattaat cccaactatc
     4321 ttccgttaca tcactacttg ggaaaatgaa tttttagact ctcaaagggt gtgggccgag
     4381 tatgcaacga aaaggcagga agctattcag cagaatagga ggttagcttt tgaagaactt
     4441 gaaggatcgt gggatcgtgg tatacctcgt attagcactt tatttcaaag agatcgacat
     4501 actctagcgt atgacagagg tcacagaatc cgtagggaat ttaaacagta ctctctagag
     4561 agaaacagcc ccttttggtg gacaaactcc caccatgacg gtaaattatg gaatttaaat
     4621 gcctatagaa ctgatgtgat tcaagcccta ggaggtattg aaacaatttt ggaacacact
     4681 ttatttaagg ggactggttt taattcatgg gaaggtttgt tttgggaaaa ggcgtccggg
     4741 tttgaggatt ccatgcaatt taagaagcta actcatgctc aaagaactgg tttaagccaa
     4801 attccaaatc gtagatttac cttatggtgg tcaccaacta ttaacagagc aaatgtctat
     4861 gtcggtttct tggtgcaatt ggatctgact ggtatcttcc tccatggtaa aattcctacg
     4921 ttaaaaatat ctttaattca aatattccgt gctcatttgt ggcaaaaaat acatgaaagt
     4981 atagtttttg atatttgtca aattctcgat ggtgagctag atgttttgca aattgaatct
     5041 gtgactaaag aaacagtgca tccccgcaaa tcgtacaaga tgaattcatc tgccgcggac
     5101 ataactatgg aaagtgttca tgaatgggaa gtttcaaaac cctccctatt acatgaaaca
     5161 aatgactcat ttaagggatt gattaccaac aaaatgtggt ttgatgtaca attgagatac
     5221 ggtgattatg attctcatga tatatctcgt tatgtgaggg caaaattcct cgattataca
     5281 acagataatg tcagcatgta tccctcccca acaggtgtta tgatcggtat cgatttggct
     5341 tataacatgt atgatgccta tggtaattgg ttcaacggat taaaaccttt gatacaaaat
     5401 agtatgagaa caattatgaa agctaaccca gcattatatg tccttcgtga acgtattaga
     5461 aaaggtcttc aaatatatca atctagtgta caggaaccat ttttaaactc ctcaaactat
     5521 gccgagttat tcaataacga cattaaactc ttcgttgacg acactaatgt gtatagagtt
     5581 actgtccaca agacttttga agggaacgtt gctacaaaag caattaatgg ttgcattttc
     5641 actttaaatc caaagactgg gcatctgttt ctgaaaatta tccatacctc tgtatgggcc
     5701 ggtcaaaaac gtttaagcca attggccaaa tggaaaacag ctgaggaagt tagtgctctt
     5761 gtgagatctt tgcccaaaga agagcagcca aaacaaatta tcgtcacaag aaaggcaatg
     5821 cttgatcctt tagaggttca catgcttgac tttcctaaca tagcaatcag gccaacagag
     5881 ctgcgactac cattttcagc tgcaatgtca atagataaac tttctgatgt tgtgatgaaa
     5941 gctactgaac ctcagatggt tctgtttaac atttatgatg attggttaga ccgtatttca
     6001 tcgtacactg cattttctag acttacacta cttttaagag ctttaaagac gaacgaggaa
     6061 agtgccaaaa tgattctact cagtgacccc acgatcacta tcaaatcgta ccatttatgg
     6121 ccttcattca ctgatgaaca atggatcact atcgaatctc aaatgagaga tttgattttg
     6181 actgagtacg gcagaaaata caatgtcaat atatctgcct taactcaaac ggaaattaaa
     6241 gatattatat tgggtcaaaa tatcaaggca ccatctgtga agagacaaaa aatggctgaa
     6301 ttagaagctg ccaggtcaga aaagcagaac gacgaagaag ctgcaggtgc atcaactgtt
     6361 atgaaaacta aaactatcaa tgcgcaaggt gaggagatag ttgtagtagc ttccgctgat
     6421 tacgaaagtc aaacgtttag ttcaaagaat gaatggagaa aatctgcaat tgctaataca
     6481 ctattatatt tgaggttaaa aaacatttat gtttcggctg atgatttcgt ggaggaacag
     6541 aatgtttacg tccttccaaa aaatttatta aagaagttta ttgagatatc cgatgtgaaa
     6601 atccaggtgg cggcctttat ttatggtatg tcagcaaaag atcatccgaa ggtgaaagaa
     6661 atcaagactg ttgtgctggt acctcaatta ggccacgtag gatccgttca gataagcaac
     6721 atccctgaca tcggagacct tccggatact gaaggccttg aactactcgg ttggatccat
     6781 acccagacag aagaacttaa attcatggct gcgtctgaag tagcaactca ttctaaactt
     6841 tttgcggata agaaaagaga ctgcattgat atttcaattt tttccacccc gggatcagta
     6901 tcattaagcg cttataacct gaccgacgaa ggttaccaat ggggtgaaga aaataaggat
     6961 ataatgaacg tgctatcgga agggtttgag cctactttca gcacgcatgc ccaattactt
     7021 ctctctgacc gtatcacagg taattttata attccatctg gaaacgtttg gaactacaca
     7081 tttatgggaa cagcatttaa ccaagaaggt gactacaatt ttaaatacgg cattcctttg
     7141 gaattctata atgagatgca tcgtccagta cactttttac agtttagcga attggcgggg
     7201 gacgaagagt tagaggccga acaaatcgat gtatttagct gaatatattc cccaataaac
     7261 tggaatctgt tatttcatag atatatatgt aagtataagt ttgtatgtat aatattcaac
     7321 gcatttttta tgcccgtcgt agttgctacc taccacacga tgatgtatta taatttcgga
     7381 actacttcaa agctacgctt tccgcactat gaaaagctat aagtatgtga aaattggtca
     7441 ttgactgtga ttctatttca aaaagtaacc aaaggagata gacatcgttc ggacagaaaa
     7501 tggtccgttg tcactcccat tccattatgt ccttcatata gaaattggat attcttgttt
     7561 tcatttcgcg caataaagca attccgtgcg gcagaaagaa cttatatata tttaactgat
     7621 cttacgctat ttatggcaaa acttgtgtta catttttgaa gataaagtta caatcattac
     7681 ggcagcctca aaacaaaatt gggagaaaac atactcaagt gagtactcat tttgtgcaag
     7741 caaacactga caattgaaga gatcgtcagg atgcccggaa cgccacagaa gaacaagagg
     7801 tctgcgagta tatctgtttc acctgcgaag aagacagagg aaaaagaaat aatacaaaat
     7861 gattcaaagg cgatattatc taagcaaact aagaggaaaa aaaagtatgc ttttgctcct
     7921 ataaataact taaatgggaa gaacaccaag gtatccaatg caagtgtcct aaagtcaata
     7981 gccgtttcac aagtacgaaa tacatcaaga acaaaagata taaacaaagc agttagcaag
     8041 agcgtaaagc aattaccaaa ttcacaagtt aaaccgaaac gggaaatgtc taatttgagt
     8101 aggcatcacg atttcactca agacgaggac ggccccatgg aagaagtaat atggaaatat
     8161 tcacctttac aacgagatat gtccgacaaa acaacaagcg ctgccgaata ctctgatgac
     8221 tacgaagatg tccaaaatcc ctcttctaca cctatagtcc ctaatcgact gaaaactgtt
     8281 cttagtttca caaatatcca agtacccaac gctgacgtaa atcaactcat tcaagaaaat
     8341 ggaaatgagc aagtgcgtcc taaaccagca gaaatatcta ccagggagtc tctccgtaat
     8401 atagatgata ttctcgatga tatagagggc gatttaacta taaaaccgac gataacgaaa
     8461 ttcagcgatt tgccatcttc acccatcaag gcacccaacg ttgaaaaaaa agcagaggtg
     8521 aatgcagaag aagtagataa aatggattca acaggagata gcaacgatgg cgatgactct
     8581 ttgatagata ttttaactca aaagtatgtt gagaaacgca agagtgagag tcagataaca
     8641 attcaaggca acaccaatca aaaaagtgga gcccaggaaa gttgtgggaa gaatgataac
     8701 acaaaatcga gaggagaaat tgaagatcat gaaaatgtag acaatcaagc caaaacaggc
     8761 aatgcatttt atgagaatga agaagacagt aattgccaaa gaattaaaaa aaatgaaaaa
     8821 atagaataca attcttcaga cgaattttca gacgattcac taattgaact attgaacgaa
     8881 actcaaacac aagtggaacc taacactatt gaacaagatt tagacaaagt ggagaaaatg
     8941 gtctctgacg atttgagaat tgctactgat tccactttat ccgcatatgc tctcagagct
     9001 aagtctggtg ctccaagaga cggcgtagta cgattagtaa tagtaagctt acctccaatt
     9061 cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg
     9121 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     9181 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     9241 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     9301 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     9361 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     9421 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     9481 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     9541 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     9601 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     9661 atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
     9721 cttacgcatc tgtgcggtat ttcacaccgc atagatccgt cgagttcaag agaaaaaaaa
     9781 agaaaaagca aaaagaaaaa aggaaagcgc gcctcgttca gaatgacacg tatagaatga
     9841 tgcattacct tgtcatcttc agtatcatac tgttcgtata catacttact gacattcata
     9901 ggtatacata tatacacatg tatatatatc gtatgctgca gctttaaata atcggtgtca
     9961 ctacataaga acacctttgg tggagggaac atcgttggta ccattgggcg aggtggcttc
    10021 tcttatggca accgcaagag ccttgaacgc actctcacta cggtgatgat cattcttgcc
    10081 tcgcagacaa tcaacgtgga gggtaattct gctagcctct gcaaagcttt caagaaaatg
    10141 cgggatcatc tcgcaagaga gatctcctac tttctccctt tgcaaaccaa gttcgacaac
    10201 tgcgtacggc ctgttcgaaa gatctaccac cgctctggaa agtgcctcat ccaaaggcgc
    10261 aaatcctgat ccaaaccttt ttactccacg cacggcccct agggcctctt taaaagcttg
    10321 accgagagca atcccgcagt cttcagtggt gtgatggtcg tctatgtgta agtcaccaat
    10381 gcactcaacg attagcgacc agccggaatg cttggccaga gcatgtatca tatggtccag
    10441 aaaccctata cctgtgtgga cgttaatcac ttgcgattgt gtggcctgtt ctgctactgc
    10501 ttctgcctct ttttctggga agatcgagtg ctctatcgct aggggaccac cctttaaaga
    10561 gatcgcaatc tgaatcttgg tttcatttgt aatacgcttt actagggctt tctgctctgt
    10621 catctttgcc ttcgtttatc ttgcctgctc attttttagt atattcttcg aagaaatcac
    10681 attactttat ataatgtata attcattatg tgataatgcc aatcgctaag aaaaaaaaag
    10741 agtcatccgc taggtggaaa aaaaaaaatg aaaatcatta ccgaggcata aaaaaatata
    10801 gagtgtacta gaggaggcca agagtaatag aaaaagaaaa ttgcgggaaa ggactgtgtt
    10861 atgacttccc tgactaatgc cgtgttcaaa cgatacctgg cagtgactcc tagcgctcac
    10921 caagctctta aaacgggaat ttatggtgca ctctcagtac aatctgctct gatgccgcat
    10981 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
    11041 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
    11101 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
    11161 aggttaatgt catgataata atggtttctt agggtccttt tcatcacgtg ctataaaaat
    11221 aattataatt taaatttttt aatataaata tataaattaa aaatagaaag taaaaaaaga
    11281 aattaaagaa aaaatagttt ttgttttccg aagatgtaaa agactctagg gggatcgcca
    11341 acaaatacta ccttttatct tgctcttcct gctctcaggt attaatgccg aattgtttca
    11401 tcttgtctgt gtagaagacc acacacgaaa atcctgtgat tttacatttt acttatcgtt
    11461 aatcgaatgt atatctattt aatctgcttt tcttgtctaa taaatatata tgtaaagtac
    11521 gctttttgtt gaaatttttt aaacctttgt ttattttttt ttcttcattc cgtaactctt
    11581 ctaccttctt tatttacttt ctaaaatcca aatacaaaac ataaaaataa ataaacacag
    11641 agtaaattcc caaattattc catcattaaa agatacgagg cgcgtgtaag ttacaggcaa
    11701 gcgatccgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
    11761 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    11821 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    11881 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    11941 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    12001 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    12061 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    12121 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    12181 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    12241 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    12301 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    12361 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    12421 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    12481 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    12541 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    12601 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    12661 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    12721 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    12781 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    12841 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    12901 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    12961 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    13021 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    13081 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    13141 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    13201 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    13261 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    13321 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    13381 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    13441 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    13501 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    13561 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    13621 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    13681 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    13741 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    13801 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    13861 accatgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggtaccgg
    13921 gccccccctc gagacttcaa agcatggttt ggtttgggcc aggcttatgc tctcctggac
    13981 atgcatttat attctcttta ctacttccag aaagcttgca ctttgaaacc ttgggatcgt
    14041 cggatttggc aagtattggg agaatgttat agtaagacgg gaaataaggt agaagctata
    14101 aaatgctaca aaagatccat aaaagcttca caaacggtcg atcaaaatac ttcaatatat
    14161 taccggttag cgcaactata tgaagaactt gaagacttgc aagaatgtaa gaagttcatg
    14221 atgaaatgtg tagatgtgga agaacttctg gaaggtatag taacagatga aaccgtgaag
    14281 gctaggcttt ggctggcaat atttgagatt aaggcaggaa actaccaatt ggcttatgat
    14341 tatgccatgg gggtatctag tggaacgtct caagagattg aagaggctcg tatgctggct
    14401 cgggagtgca gaaggcatat gtagtgaagt gaacatacac atagctattc gtactaaatg
    14461 atatgaaatt tttataaatg ccaggctata tagctattta aagtgaccat ggcagaagga
    14521 tgaaccgagg taatacggct agtacaaaag caacaaagtt aggaatacaa tttgagaaac
    14581 gaagaccata gaaaatactt gtgcgattga acttccttcc aaaaaaaaaa tagcgtcaaa
    14641 gaaag
//
