LOCUS       sequence-178507-        3238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Gateway entry clone containing endothelial specific enhancer 
            sequence of nrp1b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND
  TITLE     Robust Identification of Developmentally Active Endothelial 
            Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq.
  JOURNAL   Cell Rep. 2017 Jul 18;20(3):709-720. doi: 
            10.1016/j.celrep.2017.06.070.
  PUBMED    28723572
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-07-18- 18"; volume: "20"; issue: "3"; pages: "709-720"
COMMENT     Sequence Label: p5Enrp1bE1
FEATURES             Location/Qualifiers
     source          1..3238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      5..593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     494..513
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     747..764
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(923..950)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1042..1128)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1177..1199
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1191..1208
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1192..1208
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1248..1343
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1939..2062
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2134..2153)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2135..2153)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2158..2174)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2158..2174)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2276..2295)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2287..3096
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2354..2373)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
       61 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      121 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      181 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      241 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      301 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      361 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      421 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      481 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      541 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      601 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      661 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      721 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      781 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      841 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
      901 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
      961 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     1021 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     1081 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
     1141 tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga
     1201 cggccagtct taagctcggg cccgagttaa cgctaccatg gagctccaaa taatgatttt
     1261 attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct tttttataat
     1321 gccaactttg tatagaaaag ttggggatgt gctgaaaatg tctctaacgc acctatgtga
     1381 cgtgctttaa ctaggttaac gatggtgaac ggtcggcacg gctcattaat aggccttcac
     1441 gtccacttaa agatggctcc atcagaaaga gaggtagaag agtccttgta acgtccttct
     1501 ccctcgttcc tccatttgtc ttctagtttt atcttgctca agctgaaggg gaagccctgc
     1561 gctgcgggac cgccgctgtt ttaccttcga cccaccgcta tatttatccc attgtgctga
     1621 actaattgct ggtctgacat cacctcttga agaaggacag ctgatgaaca aagcatctgc
     1681 ggattatttt tagtttgttc ttaagacgga gcttcaggga cacacaggca gagagagaga
     1741 gagacaggaa gtgctcgtgc agctggagac aatgttatct tggtagcagg acaggaaatg
     1801 gggtccaatc acttcctggg gcctgagagc tttgctaaga cactggtttg agttctgatc
     1861 ggctgttttt ccacctcaag ggtttcagca caagggggtt tgagaagtag ctttttagtg
     1921 ctgataatca gatttagtca agtttgtaca aaaaagttga acgagaaacg taaaatgata
     1981 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     2041 cacaacatat gcagtcacta tgaaccaact acttagatgg tattagtgac ctgtagaatt
     2101 cgagctctag agctgcaggg cggccgcgat atcccctata gtgagtcgta ttacatggtc
     2161 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     2221 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     2281 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     2341 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     2401 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     2461 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     2521 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2581 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2641 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2701 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2761 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2821 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2881 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2941 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     3001 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     3061 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     3121 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     3181 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggat
//
