LOCUS       sequence-178512-        3243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Gateway entry clone containing endothelial specific enhancer 
            sequence of snx5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND
  TITLE     Robust Identification of Developmentally Active Endothelial 
            Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq.
  JOURNAL   Cell Rep. 2017 Jul 18;20(3):709-720. doi: 
            10.1016/j.celrep.2017.06.070.
  PUBMED    28723572
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-07-18- 18"; volume: "20"; issue: "3"; pages: "709-720"
COMMENT     Sequence Label: p5Esnx5E1
FEATURES             Location/Qualifiers
     source          1..3243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      10..598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     499..518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     752..769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(928..955)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1047..1133)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1182..1204
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1196..1213
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1197..1213
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1253..1348
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1949..2072
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2144..2163)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2145..2163)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2168..2184)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2168..2184)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2286..2305)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2297..3106
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2364..2383)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
       61 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      121 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      241 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      301 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      361 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      421 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      481 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      541 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      601 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      661 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      721 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      781 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      841 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      901 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      961 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
     1021 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
     1081 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
     1141 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
     1201 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
     1261 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
     1321 ataatgccaa ctttgtatag aaaagttgta ttcctggaat atgaccagga tgtgagtatt
     1381 ctgctttgtc caagccaggc ctattcttct cagaccagga aacagagaga cagtgagagg
     1441 gactgttggc attgcttatt tcctgcgaag ctgcaatgtt ggcagtttaa atacaaatgg
     1501 ggagaggttg gaggatgact gcagaagata aagagggagg gcatatttta ctcagagaac
     1561 cacttcctgc ttagttcagc gtaaaaaaaa aaagattcat gactggacta ttgcttaaaa
     1621 aaactttaaa actaagcctt aatctgtaag tcttgtatta ttcagataag tgtctgttat
     1681 gctgataaca aagacaagtg acctttttgg atctgagcca gtggtgataa agagcacaca
     1741 ctatacttct gcattctgat tggttcatat tgtgcgtgac tctgtaatcc tcttctcatt
     1801 tgggctttag ctcagcgtga ggcggaaaaa tgccaaggaa atgtttggtg gtttctttaa
     1861 gaacatggtg aagaccgctg atgaggtcct catctcaggg gtcaaggtta gtgcatcatg
     1921 cagctctctt tggggtgttt ttaaaatcca agtttgtaca aaaaagttga acgagaaacg
     1981 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
     2041 tactgtaaaa cacaacatat gcagtcacta tgaaccaact acttagatgg tattagtgac
     2101 ctgtagaatt cgagctctag agctgcaggg cggccgcgat atcccctata gtgagtcgta
     2161 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2221 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     2281 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     2341 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     2401 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     2461 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     2521 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     2581 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     2641 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     2701 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     2761 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     2821 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     2881 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     2941 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3001 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3061 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3121 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     3181 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3241 caa
//
