LOCUS       sequence-178515-        3151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Gateway entry clone containing endothelial specific enhancer 
            sequence of clec14a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND
  TITLE     Robust Identification of Developmentally Active Endothelial 
            Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq.
  JOURNAL   Cell Rep. 2017 Jul 18;20(3):709-720. doi: 
            10.1016/j.celrep.2017.06.070.
  PUBMED    28723572
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-07-18- 18"; volume: "20"; issue: "3"; pages: "709-720"
COMMENT     Sequence Label: p5Eclec14aE1
FEATURES             Location/Qualifiers
     source          1..3151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     501..520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     754..771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(930..957)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1049..1135)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1184..1206
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1198..1215
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1199..1215
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1255..1350
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1859..1982
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2054..2073)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2055..2073)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2078..2094)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2078..2094)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2196..2215)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2207..3016
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2274..2293)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
       61 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      121 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      181 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      241 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      301 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      361 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      421 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      481 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      541 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      601 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      661 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      721 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      781 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      841 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
      901 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
      961 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     1021 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     1081 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     1141 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     1201 aaaacgacgg ccagtcttaa gctcgggccc gagttaacgc taccatggag ctccaaataa
     1261 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttt
     1321 ttataatgcc aactttgtat agaaaagttg tcattgttgt ttaaaaaagc agcgagctct
     1381 cgtaataaaa cacctgtatc accttcattt caactttcct tctttgttct ttctaggcat
     1441 acttgtagct ccttttcctg ttacccataa ttccatttcc tgtccttctt ccccaccccc
     1501 ctggctctct agactgcaga acagagagtc gagtgctcct gcaacctcac ttcctgccat
     1561 tcagagccgt ttcctccagg aagcatctgc tgctctctca tgaagcggcc tgttggagga
     1621 acagaataat aatacggtgt tagtcatggg acattcaaca ccacattagt cccatgtgag
     1681 ctcttgaaaa cacacgcgga ggtcaatcaa cacgcatttg aaactgacag cgttgagtgc
     1741 gacatgtaaa cacagaggta tagtactact gtaaagaaca atccctgatg taatgagcaa
     1801 cgcttcctgg tatttttccc agagttgttt tgttacagac acaatgaaca agtcaaagca
     1861 agtttgtaca aaaaagttga acgagaaacg taaaatgata taaatatcaa tatattaaat
     1921 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat gcagtcacta
     1981 tgaaccaact acttagatgg tattagtgac ctgtagaatt cgagctctag agctgcaggg
     2041 cggccgcgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2101 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2161 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     2221 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     2281 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
     2341 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     2401 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     2461 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
     2521 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     2581 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     2641 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     2701 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     2761 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     2821 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     2881 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     2941 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     3001 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct
     3061 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     3121 ccactgagcg tcagaccccg tagaaaagat c
//
