LOCUS       sequence-178519-        3512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Gateway entry clone containing endothelial specific enhancer 
            sequence of yrk.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND
  TITLE     Robust Identification of Developmentally Active Endothelial 
            Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq.
  JOURNAL   Cell Rep. 2017 Jul 18;20(3):709-720. doi: 
            10.1016/j.celrep.2017.06.070.
  PUBMED    28723572
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-07-18- 18"; volume: "20"; issue: "3"; pages: "709-720"
COMMENT     Sequence Label: p5EyrkE1
FEATURES             Location/Qualifiers
     source          1..3512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     493..512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     746..763
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(922..949)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1041..1127)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1176..1198
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1190..1207
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1191..1207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1247..1342
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    2211..2335
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2407..2426)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2408..2426)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2431..2447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2431..2447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2549..2568)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2560..3369
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2627..2646)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
       61 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
      121 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
      181 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      241 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      301 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      361 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      421 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      481 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      541 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      601 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      661 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      721 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      781 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      841 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca
      901 ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga
      961 tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg
     1021 ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
     1081 ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt
     1141 ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac
     1201 ggccagtctt aagctcgggc ccgagttaac gctaccatgg agctccaaat aatgatttta
     1261 ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt ttttataatg
     1321 ccaactttgt atagaaaagt tgcgttctca gtactttaaa gagcctatga caacaacaac
     1381 aacaacaaca acatcatgca tatttaatgt cacagtatat tgcagcattg catactggac
     1441 taaaaagcat gatgaggttt gattaattaa atggaaaaca gaatggaatg tctctaggac
     1501 tagattaggc cataggcttc atcacttcct gttctgtgca aggtcacttc ctgtctactc
     1561 tcccctggcg ctatgggagg aagtgggtgc ggctgtccgg ttaaattgtc cgaaatgcaa
     1621 catggttaaa ctgaccaaac cagagcctgc aatgtggatt ttcagctctc cgatgttctg
     1681 ttcactactg acctgatgtt tagtgtgtag gaaacgttgg ctaaatccca catattgtat
     1741 tcaatgggtt gcttgtttga ttagcgtgaa actggcattg attctgatga tgaatacatt
     1801 tttttggctt tagttttatc caattagtgc atgcatattc agagaaagta agtttaaaaa
     1861 gcacatatat ttagtttatt ttccgaaagc acccgtgctg aaatactgcg ttcattaacg
     1921 gcctgctcgt ggaatctggc tgtggaggaa attgacatca tgtggaaaaa ggaagtggct
     1981 gtgtttgtga cacctgactt tctgttccca gggcacactc tgctattgtc ttccctctct
     2041 gcaatattat ctctcagttt ctctctcact gcattgtttc acctagttgt tgttgttgtt
     2101 gtttatttgc ctgagtgact tttattacag tttttatgag tgtgtgggtg tattcagcat
     2161 ggtctcacat tttcctgttg tgataattgc taaatcttta atcagggtat acaagtttgt
     2221 acaaaaaagt tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
     2281 tgcataaaaa acagactaca taatactgta aaacacaaca tatgcagtca ctatgaacca
     2341 actacttaga tggtattagt gacctgtaga attcgagctc tagagctgca gggcggccgc
     2401 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     2461 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     2521 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     2581 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     2641 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     2701 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     2761 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     2821 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     2881 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     2941 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     3001 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3061 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     3121 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3181 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     3241 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     3301 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     3361 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     3421 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     3481 gcgtcagacc ccgtagaaaa gatcaaagga tc
//
