LOCUS       sequence-178524-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to make Fosmids that can be converted into Illumina sequencable 
            libraries.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Williams LJ, Tabbaa DG, Li N, Berlin AM, Shea TP, Maccallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A
  TITLE     Paired-end sequencing of Fosmid libraries by Illumina.
  JOURNAL   Genome Res. 2012 Nov;22(11):2241-9. doi: 10.1101/gr.138925.112. Epub
            2012 Jul 16.
  PUBMED    22800726
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.138925.112"; journalName: "Genome Res"; date: "2012-11";
            volume: "22"; issue: "11"; pages: "2241-9"
COMMENT     Sequence Label: pFosill-1
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     153..170
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(351..370)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(352..370)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    386..419
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc
       61 tgaataagtg ataataagcg gatgaatggc agaaattcga tgataagctg tcaaacatga
      121 gaattggtcg acggcccggg cggccgcata cgatttaggt gacactatag gatctcaagc
      181 agaagacggc atacgacact gctgaggatc ggtctcggca ttcctgctga accgctcttc
      241 cgatctccac gtgcatgctg gatccagcat gcacgtggag atcggaagag cgtcgtgtag
      301 ggaaagagtg tcctcagcag tgtcggtggt cgccgtatca ttaagcttct ccctatagtg
      361 agtcgtatta gcggccgcat cgaatataac ttcgtataat gtatgctata cgaagttatt
      421 agcgatgagc tcggacttcc attgttcatt ccacggacaa aaacagagaa aggaaacgac
      481 agaggccaaa aagctcgctt tcagcacctg tcgtttcctt tcttttcaga gggtatttta
      541 aataaaaaca ttaagttatg acgaagaaga acggaaacgc cttaaaccgg aaaattttca
      601 taaatagcga aaacccgcga ggtcgccgcc ccgtaacctg tcggatcacc ggaa
//
