LOCUS       sequence-178526-        3236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Gateway entry clone containing endothelial specific enhancer 2 
            sequence of dll4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND
  TITLE     Robust Identification of Developmentally Active Endothelial 
            Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq.
  JOURNAL   Cell Rep. 2017 Jul 18;20(3):709-720. doi: 
            10.1016/j.celrep.2017.06.070.
  PUBMED    28723572
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-07-18- 18"; volume: "20"; issue: "3"; pages: "709-720"
COMMENT     Sequence Label: p5Edll4E2
FEATURES             Location/Qualifiers
     source          1..3236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     496..515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     749..766
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(925..952)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1044..1130)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1179..1201
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1193..1210
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1194..1210
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1250..1345
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1939..2062
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2134..2153)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2135..2153)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2158..2174)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2158..2174)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2276..2295)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2287..3096
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2354..2373)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
       61 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
      121 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
      181 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
      241 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
      301 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      361 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      421 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      481 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      541 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      601 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      661 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      721 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
      781 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      841 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag
      901 ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt
      961 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca
     1021 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     1081 cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca
     1141 gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac
     1201 gacggccagt cttaagctcg ggcccgagtt aacgctacca tggagctcca aataatgatt
     1261 ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg cttttttata
     1321 atgccaactt tgtatagaaa agttgttgtt gtacatttac atgtcaaaaa tttggctttc
     1381 cattcatgag aaacacataa gtcaggtcca aaaacccgcc agtttgctgt ctggtgagaa
     1441 accaggaagt aacaaagcac tgaagatcaa tgagagcgag gcggtgacag gaaggggagg
     1501 agctagagcc gtccaatgac aggcaggcct cagggagcag caactgacgc aaaccaggaa
     1561 gccacctgat gtgactgggg tcagataaac gcaggccgcg gaagaacagg tgcttcctct
     1621 gcgctcgctg ccttcttcgg ccatgtcaga ctggtgtgtg tgtgtgtgta tttgtgtgtg
     1681 tgcacatgag tgtgtatgtg cgtgtgtgga gcgtttaaag gaacagcatc ctcgggcggg
     1741 tcgagacgaa ggacagtgca cgcgcacagg ccaggaaacg ccgcacaact ccggctgttt
     1801 ttgtttttgt tcagccggtc tgtttctata tacatgctgt aaacacacat ttactcttcg
     1861 aaaaaagaag aagtcgttca gaattactct tcaaggactc aacctcatct gctcctaata
     1921 taggcacttg tcaaaaagca agtttgtaca aaaaagttga acgagaaacg taaaatgata
     1981 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     2041 cacaacatat gcagtcacta tgaaccaact acttagatgg tattagtgac ctgtagaatt
     2101 cgagctctag agctgcaggg cggccgcgat atcccctata gtgagtcgta ttacatggtc
     2161 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     2221 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     2281 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     2341 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     2401 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     2461 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     2521 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2581 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2641 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2701 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2761 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2821 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2881 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2941 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     3001 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     3061 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     3121 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     3181 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaagg
//
