LOCUS       sequence-178531-         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to make Fosmids that can be converted into Illumina sequencable 
            libraries.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Williams LJ, Tabbaa DG, Li N, Berlin AM, Shea TP, Maccallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A
  TITLE     Paired-end sequencing of Fosmid libraries by Illumina.
  JOURNAL   Genome Res. 2012 Nov;22(11):2241-9. doi: 10.1101/gr.138925.112. Epub
            2012 Jul 16.
  PUBMED    22800726
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.138925.112"; journalName: "Genome Res"; date: "2012-11";
            volume: "22"; issue: "11"; pages: "2241-9"
COMMENT     Sequence Label: pFosill-2
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     153..170
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(348..367)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(349..367)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    383..416
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc
       61 tgaataagtg ataataagcg gatgaatggc agaaattcga tgataagctg tcaaacatga
      121 gaattggtcg acggcccggg cggccgcata cgatttaggt gacactatag gatctcaagc
      181 agaagacggc atacgacact gctgaggatg tgactggagt tcagacgtgt gctcttccga
      241 tctccacgtg catgctggat ccagcatgca cgtggagatc ggaagagcgt cgtgtaggga
      301 aagagtgtcc tcagcagtgt cggtggtcgc cgtatcatta agcttctccc tatagtgagt
      361 cgtattagcg gccgcatcga atataacttc gtataatgta tgctatacga agttattagc
      421 gatgagctcg gacttccatt gttcattcca cggacaaaaa cagagaaagg aaacgacaga
      481 ggccaaaaag ctcgctttca gcacctgtcg tttcctttct tttcagaggg tattttaaat
      541 aaaaacatta agttatgacg aagaagaacg gaaacgcctt aaaccggaaa attttcataa
      601 atagcgaaaa cccgcgaggt cgccgccccg taacctgtcg gatcaccgga aaggacccgt
      661 aa
//
