LOCUS       sequence-17854-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Scheffner M, Huibregtse JM, Vierstra RD, Howley PM
  TITLE     The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein 
            ligase in the ubiquitination of p53.
  JOURNAL   Cell. 1993 Nov 5. 75(3):495-505.
  PUBMED    8221889
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1993 
            Nov 5. 75(3):495-505."
COMMENT     Sequence Label: p4027 GST-ubiquitin
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             46..270
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(332..354)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(499..517)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        585..689
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(908..927)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcggatctgg ttccgcgtgg atctcgtcgt gcatctgttg gatcccagat tttcgtcaag
       61 actttgaccg gtaaaaccat aacattggaa gttgaatctt ccgataccat cgacaacgtt
      121 aagtcgaaaa ttcaagacaa ggaaggtatc cctccagatc aacaaagatt gatctttgcc
      181 ggtaagcagc tagaagacgg tagaacgctg tctgattaca acattcagaa ggagtccacc
      241 ttacatcttg tgctaaggct aagaggtggt tgagaattca tcgtgactga ctgacgatct
      301 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
      361 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
      421 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
      481 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      541 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      601 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      661 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      721 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      781 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      841 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      901 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggc
//
