LOCUS       sequence-178573-        8320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary DEST vector for plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Faden F, Ramezani T, Mielke S, Almudi I, Nairz K, Froehlich MS, 
            Hockendorff J, Brandt W, Hoehenwarter W, Dohmen RJ, Schnittger A, 
            Dissmeyer N
  TITLE     Phenotypes on demand via switchable target protein degradation in 
            multicellular organisms.
  JOURNAL   Nat Commun. 2016 Jul 22;7:12202. doi: 10.1038/ncomms12202.
  PUBMED    27447739
REFERENCE   2  (bases 1 to 8320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-22"; volume: "7:12202. doi"; pages: " 
            10.1038/ncomms12202"
COMMENT     Sequence Label: pAM-PAT-ProUBQ10 GW
FEATURES             Location/Qualifiers
     source          1..8320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    348..372
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      484..1193
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1266..1357
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1358..1426,1427..2218)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1576..1595)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2389..2977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2878..2897
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3289..3313
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3407..3659)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3679..4230)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4272..4451)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    6593..6717
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6742..6747,6748..6765,6766..6772)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6826..7485
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6892..6911)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7827..8132
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8011..8030
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8173..8297)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 agcttgttga gaattcatcg cccgggactg ggttaacttc cgatcgattc tagactagtt
       61 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
      121 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
      181 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
      241 taaaatttct aattcctaaa accaaaatcc agtgaccggg cggccgccac cgcggtggag
      301 ggggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
      361 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
      421 tgaaaaggtt tatccgttcg tccatttgta tgtgtacatc accgacgagc aaggcaagac
      481 cgagcgcctt tccgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct
      541 atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cggccgccgg
      601 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
      661 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
      721 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
      781 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
      841 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
      901 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
      961 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
     1021 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
     1081 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc
     1141 cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc
     1201 agtccttgcc attgccggga tcggggcagt aacgggatgg gcgatcagcc cgaaagctac
     1261 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1321 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     1381 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     1441 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     1501 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     1561 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     1621 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     1681 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     1741 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1801 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     1861 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     1921 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1981 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2041 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     2101 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2161 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     2221 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2281 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     2341 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2401 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2461 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2521 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2581 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2641 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2701 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2761 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2821 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2881 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2941 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3001 acggttcctg gccttttgct ggccttttgc tcacatggac tctagctaga ggatcacagg
     3061 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
     3121 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
     3181 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
     3241 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
     3301 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
     3361 gatcgaatac taacgtctct accagatatc agcttgcatg ccggtcgatc tagtaacata
     3421 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
     3481 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
     3541 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
     3601 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct
     3661 gcttgactct aggggtcatc agatttcggt gacgggcagg accggacggg gcggcaccgg
     3721 caggctgaag tccagctgcc agaaacccac gtcatgccag ttcccgtgct tgaagccggc
     3781 cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca cgctcgggtc
     3841 gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca gggacttcag
     3901 caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg agacgtacac
     3961 ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc cagggacccg cgtaggcgat
     4021 gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc gcagacggac
     4081 gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga ccgtgcttgt
     4141 ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg tggcacggcg
     4201 gatgtcggcc gggcgtcgtt ctgggctcat ggtagatccc ctcgatcgag ttgagagtga
     4261 atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga
     4321 caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt
     4381 tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctcagtg gctccttcaa
     4441 cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc
     4501 ataacgtgac tcccttaatt ctccgctcat gatcagattg tcgtttcccg ccttcggttt
     4561 gggcgcgcca tttaaatagt ctagctcaac agagctttta acccaaattg gtacaataga
     4621 atacaacttt agatcataat tctcaaaaga aagagattcc ttagctattc tatctgccac
     4681 tccatttcct tctcggcttg tatgcacaag cataaaatcc tcaaacttgc taagtagata
     4741 ctttatgtct tggataattg gattgagact tgacaagcat aactttcatg taaccaaaga
     4801 cacaagttgc tgagaatcca cctcaaaaat gatcttccta taattgaatc gggataatga
     4861 cagcacagcc catctaagag cctccacttc tacttccagc acgcttctta cttttaccac
     4921 agctcttgca cctaaccata acaccttccc tgtatgatcg cgaagcaccc accctaagcc
     4981 acattttaat ccttctgttg gccatgcccc atcaaagttg cacttaaccc aagattgtgg
     5041 tggagcttcc catgtttctc gtctgtcccg acggtgttgt ggttggtgct ttccttacat
     5101 tctgagcctc tttccttcta atccactcat ctgcatcttc ttgtgtcctt actaatacct
     5161 cattggttcc aaattccctc cctttaagca ccagctcgtt tctgttcttc cacagcctcc
     5221 caagtatcca agggactaaa gcctccacat tcttcagatc aggatattct tgtttaagat
     5281 gttgaactct atggaggttt gtatgaactg atgatctagg accggataag ttcccttctt
     5341 catagcgaac ttattcaaag aatgttttgt gtatcattct tgttacattg ttattaatga
     5401 aaaaatatta ttggtcattg gactgaacac gagtgttaaa tatggaccag gccccaaata
     5461 agatccattg atatatgaat taaataacaa gaataaatcg agtcaccaaa ccacttgcct
     5521 tttttaacga gacttgttca ccaacttgat acaaaagtca ttatcctatg caaatcaata
     5581 atcatacaaa aatatccaat aacactaaaa aattaaaaga aatggataat ttcacaatat
     5641 gttatacgat aaagaagtta cttttccaag aaattcactg attttataag cccacttgca
     5701 ttagataaat ggcaaaaaaa aacaaaaagg aaaagaaata aagcacgaag aattctagaa
     5761 aatacgaaat acgcttcaat gcagtgggac ccacggttca attattgcca attttcagct
     5821 ccaccgtata tttaaaaaat aaaacgataa tgctaaaaaa atataaatcg taacgatcgt
     5881 taaatctcaa cggctggatc ttatgacgac cgttagaaat tgtggttgtc gacgagtcag
     5941 taataaacgg cgtcaaagtg gttgcagccg gcacacacga gtcgtgttta tcaactcaaa
     6001 gcacaaatac ttttcctcaa cctaaaaata aggcaattag ccaaaaacaa ctttgcgtgt
     6061 aaacaacgct caatacacgt gtcattttat tattagctat tgcttcaccg ccttagcttt
     6121 ctcgtgacct agtcgtcctc gtcttttctt cttcttcttc tataaaacaa tacccaaaga
     6181 gctcttcttc ttcacaattc agatttcaat ttctcaaaat cttaaaaact ttctctcaat
     6241 tctctctacc gtgatcaagg taaatttctg tgttccttat tctctcaaaa tcttcgattt
     6301 tgttttcgtt cgatcccaat ttcgtatatg ttctttggtt tagattctgt taatcttaga
     6361 tcgaagacga ttttctgggt ttgatcgtta gatatcatct taattctcga ttagggtttc
     6421 atagatatca tccgatttgt tcaaataatt tgagttttgt cgaataatta ctcttcgatt
     6481 tgtgatttct atctagatct ggtgttagtt tctagtttgt gcgatcgaat ttgtcgatta
     6541 atctgagttt ttctgattaa cagcctcgag aaagaggatc cacctgagga tcacaagttt
     6601 gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
     6661 tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt cactatggcg
     6721 gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt
     6781 ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     6841 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     6901 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     6961 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     7021 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     7081 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     7141 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     7201 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     7261 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     7321 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     7381 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     7441 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc
     7501 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
     7561 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
     7621 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
     7681 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
     7741 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
     7801 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
     7861 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
     7921 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
     7981 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     8041 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     8101 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
     8161 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
     8221 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
     8281 ttcttgtaca aagtggtgat cctccgttcc atgggctaga
//
