LOCUS       sequence-178596-       10153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 10153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag TBK1
FEATURES             Location/Qualifiers
     source          1..10153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        85..464
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        465..668
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     619..639
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    889..1088
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1289..1705
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1635..1657
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1673..1689
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1723..1732
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1729..1770
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1795..1818
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1825..1849
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4037..4061)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4120..4581
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4176..4193)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4403..4422
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4586..5380
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4640..4659)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5250..5269
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5567..6036
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        6072..6176
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6177..6245,6246..7037)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6395..6414)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8803..9391
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9292..9311
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9545..9562
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9666..10093)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9776..9797)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9988..10007
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
       61 catgtccaat atgaccgcca tgttggcatt gattattgac tagttattaa tagtaatcaa
      121 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      181 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      241 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      301 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg
      361 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
      421 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      481 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      541 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      601 ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      661 gcagagctgg tttagtgaac cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg
      721 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
      781 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg
      841 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggcaa gctggccagc
      901 aacttatctg tgtctgtccg attgtctagt gtctatgact gattttatgc gcctgcgtcg
      961 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
     1021 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
     1081 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
     1141 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
     1201 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
     1261 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1321 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1381 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1441 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1501 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1561 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1621 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1681 agccctcact ccttctctag gcgccggccg gatccagaat tcgccaccat ggggtcttca
     1741 aaatctaaac caaaggaccc cagccagcgc cggcgcagga tccgaggtta ccttgactac
     1801 aaagacgatg acgacaagca attgacaagt ttgtacaaaa aagttggcat gcagagcact
     1861 tctaatcatc tgtggctttt atctgatatt ttaggccaag gagctactgc aaatgtcttt
     1921 cgtggaagac ataagaaaac tggtgattta tttgctatca aagtatttaa taacataagc
     1981 ttccttcgtc cagtggatgt tcaaatgaga gaatttgaag tgttgaaaaa actcaatcac
     2041 aaaaatattg tcaaattatt tgctattgaa gaggagacaa caacaagaca taaagtactt
     2101 attatggaat tttgtccatg tgggagttta tacactgttt tagaagaacc ttctaatgcc
     2161 tatggactac cagaatctga attcttaatt gttttgcgag atgtggtggg tggaatgaat
     2221 catctacgag agaatggtat agtgcaccgt gatatcaagc caggaaatat catgcgtgtt
     2281 ataggggaag atggacagtc tgtgtacaaa ctcacagatt ttggtgcagc tagagaatta
     2341 gaagatgatg agcagtttgt ttctctgtat ggcacagaag aatatttgca ccctgatatg
     2401 tatgagagag cagtgctaag aaaagatcat cagaagaaat atggagcaac agttgatctt
     2461 tggagcattg gggtaacatt ttaccatgca gctactggat cactgccatt tagacccttt
     2521 gaagggcctc gtaggaataa agaagtgatg tataaaataa ttacaggaaa gccttctggt
     2581 gcaatatctg gagtacagaa agcagaaaat ggaccaattg actggagtgg agacatgcct
     2641 gtttcttgca gtctttctcg gggtcttcag gttctactta cccctgttct tgcaaacatc
     2701 cttgaagcag atcaggaaaa gtgttggggt tttgaccagt tttttgcaga aactagtgat
     2761 atacttcacc gaatggtaat tcatgttttt tcgctacaac aaatgacagc tcataagatt
     2821 tatatacata gctataatac tgctactata tttcatgaac tggtatataa acaaaccaaa
     2881 attatttctt caaatcaaga acttatctac gaagggcgac gcttagtctt agaacctgga
     2941 aggctggcac aacatttccc taaaactact gaggaaaacc ctatatttgt agtaagccgg
     3001 gaacctctgg ataccatagg attaatatat gaaaaaattt ccctccctaa agtacatcca
     3061 cgttatgatt tagacgggga tgctagcatg gctaaggcaa taacaggggt tgtgtgttat
     3121 gcctgcagaa ttgccagtac cttactgctt tatcaggaat taatgcgaaa ggggatacga
     3181 tggctgattg aattaattaa agatgattac aatgaaactg ttcacaaaaa gacagaagtt
     3241 gtgatcacat tggatttctg tatcagaaac attgaaaaaa ctgtgaaagt atatgaaaag
     3301 ttgatgaaga tcaacctgga agcggcagag ttaggtgaaa tttcagacat acacaccaaa
     3361 ttgttgagac tttccagttc tcagggaaca atagaaacca gtcttcagga tatcgacagc
     3421 agattatctc caggtggatc actggcagac gcatgggcac atcaagaagg cactcatccg
     3481 aaagacagaa atgtagaaaa actacaagtc ctgttaaatt gcatgacaga gatttactat
     3541 cagttcaaaa aagaccaagc agaacgtaga ttagcttata atgaagaaca aatccacaaa
     3601 tttgataagc aaaaactgta ttaccatgcc acaaaagcta tgacgcactt tacagatgaa
     3661 tgtgttaaaa agtatgaggc atttttgaat aagtcagaag aatggataag aaagatgctt
     3721 catcttagga aacagttatt atcgctgact aatcagtgtt ttgatattga agaagaagta
     3781 tcaaaatatc aagaatatac taatgagtta caagaaactc tgcctcagaa aatgtttaca
     3841 gcttccagtg gaatcaaaca taccatgacc ccaatttatc caagttctaa cacattagta
     3901 gaaatgactc ttggtatgaa gaaattaaag gaagagatgg aaggggtggt taaagaactt
     3961 gctgaaaata accacatttt agaaaggttt ggctctttaa ccatggatgg tggccttcgc
     4021 aacgttgact gtctttaccc aactttcttg tacaaagtgg tttgatctag tcgaccaatt
     4081 ccggttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     4141 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     4201 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     4261 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     4321 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     4381 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     4441 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     4501 atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     4561 ctttgaaaaa cacgatgata ataccatgat tgaacaagat ggattgcacg caggttctcc
     4621 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     4681 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     4741 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     4801 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     4861 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     4921 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     4981 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     5041 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     5101 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     5161 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     5221 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     5281 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     5341 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgca
     5401 tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     5461 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     5521 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca
     5581 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca
     5641 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     5701 agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg
     5761 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     5821 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     5881 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca
     5941 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     6001 ctgagtgatt gactacccgt cagcgggggt ctttcacagg ccagtggggc cgcacttttc
     6061 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6121 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6181 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6241 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6301 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6361 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6421 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6481 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6541 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6601 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6661 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6721 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6781 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6841 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6901 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6961 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7021 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttgc
     7081 ggccgcgaaa aggacaagca gcgaaaattc acgccccctt gggaggtggc ggcatatgca
     7141 aaggatagca ctcccactct actactgggt atcatatgct gactgtatat gcatgaggat
     7201 agcatatgct acccggatac agattaggat agcatatact acccagatat agattaggat
     7261 agcatatgct acccagatat agattaggat agcctatgct acccagatat aaattaggat
     7321 agcatatact acccagatat agattaggat agcatatgct acccagatat agattaggat
     7381 agcctatgct acccagatat agattaggat agcatatgct atccagatat ttgggtagta
     7441 tatgctaccc atggcaacat tagcccaccg tgctctcagc gacctcgtga atatgaggac
     7501 caacaaccct gtgcttggcg ctcaggcgca agtgtgtgta atttgtcctc cagatcgcag
     7561 caatcgcgcc cctatcttgg cccgcccacc tacttatgca ggtattcccc ggggtgccat
     7621 tagtggtttt gtgggcaagt ggtttgaccg cagtggttag cggggttaca atcagccaag
     7681 ttattacacc cttattttac agtccaaaac cgcagggcgg cgtgtggggg ctgacgcgtg
     7741 cccccactcc acaatttcaa aaaaaagagt ggccacttgt ctttgtttat gggccccatt
     7801 ggcgtggagc cccgtttaat tttcgggggt gttagagaca accagtggag tccgctgctg
     7861 tcggcgtcca ctctctttcc ccttgttaca aatagagtgt aacaacatgg ttcacctgtc
     7921 ttggtccctg cctgggacac atcttaataa ccccagtatc atattgcact aggattatgt
     7981 gttgcccata gccataaatt cgtgtgagat ggacatccag tctttacggc ttgtccccac
     8041 cccatggatt tctattgtta aagatattca gaatgtttca ttcctacact agtatttatt
     8101 gcccaagggg tttgtgaggg ttatattggt gtcatagcac aatgccacca ctgaaccccc
     8161 cgtccaaatt ttattctggg ggcgtcacct gaaaccttgt tttcgagcac ctcacataca
     8221 ccttactgtt cacaactcag cagttattct attagctaaa cgaaggagaa tgaagaagca
     8281 ggcgaagatt caggagagtt cactgcccgc tccttgatct tcagccactg cccttgtgac
     8341 taaaatggtt cactaccctc gtggaatcct gaccccatgt aaataaaacc gtgacagctc
     8401 atggggtggg agatatcgct gttccttagg acccttttac taaccctaat tcgatagcat
     8461 atgcttcccg ttgggtaaca tatgctattg aattagggtt agtctggata gtatatacta
     8521 ctacccggga agcatatgct acccgtttag ggttaacaag ggggccttat aaacactatt
     8581 gctaatgccc tcttgagggt ccgcttatcg gtagctacac aggcccctct gattgacgtt
     8641 ggtgtagcct cccgtagtct tcctgggccc ctgggaggta catgtcggcc gcaaaaggat
     8701 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8761 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8821 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     8881 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8941 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9001 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9061 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9121 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9181 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9241 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9301 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9361 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9421 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9481 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9541 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9601 cgcgcgttgg ccgattcatt aatgcaacta tggttggcgc gttgtaaacg ttaatatttt
     9661 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat agaccgaaat
     9721 cggcaaaatc ccttataaat caaaagaata gcccgagata gagttgagtg ttgttccagt
     9781 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     9841 ctatcagggc gatggcccac tacgtgaacc atcacccaaa tcaagttttt tggggtcgag
     9901 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     9961 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctaaggc
    10021 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
    10081 gctacagggc gcgccgatct tcaatattgg ccattagcca tattattcat tggttatata
    10141 gcataaatca ata
//
