LOCUS       sequence-178628-        9196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rosa26 targeting vector for Dre-responsive cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Lusk SJ, McKinney A, Hunt PJ, Fahey PG, Patel J, Chang A, Sun JJ, 
            Martinez VK, Zhu PJ, Egbert JR, Allen G, Jiang X, Arenkiel BR, 
            Tolias AS, Costa-Mattioli M, Ray RS
  TITLE     A CRISPR toolbox for generating intersectional genetic mouse models 
            for functional, molecular, and anatomical circuit mapping.
  JOURNAL   BMC Biol. 2022 Jan 28;20(1):28. doi: 10.1186/s12915-022-01227-0.
  PUBMED    35086530
REFERENCE   2  (bases 1 to 9196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2022-01-28"; volume: "20(1):28. doi"; pages: " 
            10.1186/s12915-022-01227-0"
COMMENT     Sequence Label: 
            p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_RNeoStop_WPRE_bgh_pA
FEATURES             Location/Qualifiers
     source          1..9196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(132..149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(266..811)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(346..365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1984..2363
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2365..2642
                     /label=chicken beta-actin promoter
     intron          2643..3652
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3576..3598
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3660..3679
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3709..3740
                     /label=rox
                     /bound_moiety="Dre recombinase"
     CDS             3741..4544
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3804..3823)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4414..4433
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          5087..5152
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5282..5302
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5727..5861
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5764..5783)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5818..5837
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    5903..5934
                     /label=rox
                     /bound_moiety="Dre recombinase"
     misc_feature    5960..6548
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6013..6033)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6431..6442)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6564..6788
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    6800..7610
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     misc_feature    7124..7773
                     /label=HA-R
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     CDS             complement(8052..8867)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8052..8867)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8775..8794
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8859..8878
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct
       61 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata
      121 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
      181 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
      241 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
      301 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
      361 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
      421 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
      481 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta
      541 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca
      601 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac
      661 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt
      721 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca
      781 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata
      841 tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg
      901 atataagttg tatttcgtcg acggaagagc ggcgcgccaa atctccgttc tgtgagacag
      961 ccgggtacga gtcgtgacgc tggaaggggc aagcgggtgg tgggcaggaa tgcggtccgc
     1021 cctgcagcaa ccggaggggg agggagaagg gagcggaaaa gtctccaccg gacgcggcca
     1081 tggctcgggg gggggggggc agcggaggag cgcttccggc cgacgtctcg tcgctgattg
     1141 gcttcttttc ctcccgccgt gtgtgaaaac acaaatggcg tgttttggtt ggcgtaaggc
     1201 gcctgtcagt taacggcagc cggagtgcgc agccgccggc agcctcgctc tgcccactgg
     1261 gtggggcggg aggtaggtgg ggtgaggcga gctggacgtg cgggcgcggt cggcctctgg
     1321 cggggcgggg gaggggaggg agggtcagcg aaagtagctc gcgcgcgagc ggccgcccac
     1381 cctccccttc ctctggggga gtcgttttac ccgccgccgg ccgggcctcg tcgtctgatt
     1441 ggctctcggg gcccagaaaa ctggcccttg ccattggctc gtgttcgtgc aagttgagtc
     1501 catccgccgg ccagcggggg cggcgaggag gcgctcccag gttccggccc tcccctcggc
     1561 cccgcgccgc agagtctggc cgcgcgcccc tgcgcaacgt ggcaggaagc gcgcgctggg
     1621 ggcggggacg ggcagtaggg ctgagcggct gcggggcggg tgcaagcacg tttccgactt
     1681 gagttgcctc aagaggggcg tgctgagcca gacctccatc gcgcactccg gggagtggag
     1741 ggaaggagcg agggctcagt tgggctgttt tggaggcagg aagcacttgc tctcccaaag
     1801 tcgctctgag ttgttatcag taagggagct gcagtggagt aggcggggag aaggccgcac
     1861 ccttctccgg aggggggagg ggagtgttgc aatacctttc tgggagttct ctgctgcctc
     1921 ctggcttctg aggaccgccc tgggcctggg agaatccctt ccccctcttc tttctttaat
     1981 taagccattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     2041 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2101 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2161 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2221 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2281 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2341 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
     2401 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     2461 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg tgcgaggggc
     2521 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
     2581 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
     2641 cgggagtcgc tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg cgccgcccgc
     2701 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     2761 cgggctgtaa ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg ctgcgtgaaa
     2821 gccttaaagg gctccgggag ggccctttgt gcggggggga gcggctcggg gggtgcgtgc
     2881 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
     2941 tgcgggcgcg gcgcggggct ttgtgcgctc cgcgtgtgcg cgaggggagc gcggccgggg
     3001 gcggtgcccc gcggtgcggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     3061 cgtggggggg tgagcagggg gtgtgggcgc ggcggtcggg ctgtaacccc cccctgcacc
     3121 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt gcggggcgtg
     3181 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     3241 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggccccgga gcgccggcgg
     3301 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     3361 gggacttcct ttgtcccaaa tctggcggag ccgaaatctg ggaggcgccg ccgcaccccc
     3421 tctagcgggc gcgggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
     3481 ttcgtgcgtc gccgcgccgc cgtccccttc tccatctcca gcctcggggc tgccgcaggg
     3541 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     3601 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     3661 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta actttaaata
     3721 attggcatta tttaaagtta atgggatcgg ccattgaaca agatggattg cacgcaggtt
     3781 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     3841 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     3901 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     3961 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     4021 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     4081 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     4141 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4201 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4261 tcgccaggct caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc catggcgatg
     4321 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     4381 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     4441 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     4501 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctaatagtga gatattaagg
     4561 gttccggatc ctcggggaca ccaaatatgg cgatctcggc cttttcgttt cttggagctg
     4621 ggacatgttt gccatcgatc catctaccac cagaacggcc gttagatctg ctgccaccgt
     4681 tgtttccacc gaagaaacca ccgttgccgt aaccaccacg acggttgttg ctaaagaagc
     4741 tgccaccgcc acggccaccg ttgtagccgc cgttgttgtt attgtagttg ctactgttat
     4801 ttctggcact tcttggtttt cctcttaagt gaggaggaac ataaccattc tcgttgttgt
     4861 cgttgatgct taaattttgc acttgttcgc tcagttcagc cataatatga aatgcttttc
     4921 ttgttgttct tacggaatac cacttgccac ctatcaccac aactaacttt ttcccgttcc
     4981 tccatctctt ttatattttt tttctcgagg gatctttgtg aaggaacctt acttctgtgg
     5041 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     5101 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     5161 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     5221 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     5281 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     5341 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     5401 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
     5461 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
     5521 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
     5581 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
     5641 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     5701 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     5761 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5821 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgacatggt aagtaagctt
     5881 gggctgcagg tcgagggacc tataacttta aataattggc attatttaaa gttaatcagg
     5941 cctgtttaaa catttaaata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6001 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6061 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6121 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6181 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6241 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6301 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     6361 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6421 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6481 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6541 ctccccgcct gaaataattc cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     6601 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     6661 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     6721 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     6781 ctctatggtt aattaaagaa gatgggcggg agtcttctgg gcaggcttaa aggctaacct
     6841 ggtgtgtggg cgttgtcctg caggggaatt gaacaggtgt aaaattggag ggacaagact
     6901 tcccacagat tttcggtttt gtcgggaagt tttttaatag gggcaaataa ggaaaatggg
     6961 aggataggta gtcatctggg gttttatgca gcaaaactac aggttattat tgcttgtgat
     7021 ccgcctcgga gtattttcca tcgaggtaga ttaaagacat gctcacccga gttttatact
     7081 ctcctgcttg agatccttac tacagtatga aattacagtg tcgcgagtta gactatgtaa
     7141 gcagaatttt aatcattttt aaagagccca gtacttcata tccatttctc ccgctccttc
     7201 tgcagcctta tcaaaaggta ttttagaaca ctcattttag ccccattttc atttattata
     7261 ctggcttatc caacccctag acagagcatt ggcattttcc ctttcctgat cttagaagtc
     7321 tgatgactca tgaaaccaga cagattagtt acatacacca caaatcgagg ctgtagctgg
     7381 ggcctcaaca ctgcagttct tttataactc cttagtacac tttttgttga tcctttgcct
     7441 tgatccttaa ttttcagtgt ctatcacctc tcccgtcagg tggtgttcca catttgggcc
     7501 tattctcagt ccagggagtt ttacaacaat agatgtattg agaatccaac ctaaagctta
     7561 actttccact cccatgaatg cctctctcct ttttctccat ttataaactg agctattaac
     7621 cattaatggt ttccaggtgg atgtctcctc ccccaatatt acctgatgta tcttacatat
     7681 tgccaggctg atattttaag acattaaaag gtatatttca ttattgagcc acatggtatt
     7741 gattactgct tactaaaatt ttgtcattgt acacatctgt aaaaggtggt tccttttgga
     7801 atgcaaagtt caggtgtttg ttgtctttcc tgacctaagg tcttgtgagc ttgtattttt
     7861 tctatttaag cagtgctttc tcttggactg gcttgactca tggcattcta cacgttattg
     7921 ctggtctaaa tgtgattttg ccaagcttct tcaggaccta taattttgct tgacttgtag
     7981 ccaaacacaa gtaaaatgat taagcaacaa atgtatttgt gaagcttggt ccaccacgct
     8041 cggagggcct gttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     8101 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     8161 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     8221 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     8281 tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt
     8341 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     8401 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     8461 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     8521 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
     8581 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     8641 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     8701 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     8761 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     8821 tttaatcgcg gcctcgagca agacgtttcc cgttgaatat ggctcataac accccttgta
     8881 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
     8941 atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct
     9001 gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa
     9061 aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg tggctccctc
     9121 actttctggc tggatgatgg ggcgattcag gcctggtatg agtcagcaac accttcttca
     9181 cgaggcagac ctcagc
//
