LOCUS       sequence-17863-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  TITLE     pRK7
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRK7
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      65..74
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    78..212
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(115..134)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     169..188
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        281..638
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        281..472
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(304..324)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      489..624
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(658..675)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(658..674)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(667..689)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ctgcacctcg gttctaagct tctgcaggtc gactctagag gatccccggg gaattcaatc
       61 gatggccgcc atggcccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      121 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      181 aactcatcaa tgtatcttat catgtctgga tcgggaatta attcggcgca gcaccatggc
      241 ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga aagaaccagc
      301 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
      361 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
      421 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
      481 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      541 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
      601 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctgttaaca gcttggcact
      661 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      721 tgcagcacat c
//
