LOCUS       sequence-178631-        5195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Surfactant Protein B Gene Promoter Reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Runkle EA, Rice SJ, Qi J, Masser D, Antonetti DA, Winslow MM, Mu D
  TITLE     Occludin is a direct target of thyroid transcription factor-1 
            (TTF-1/NKX2-1).
  JOURNAL   J Biol Chem. 2012 Aug 17;287(34):28790-801. doi: 
            10.1074/jbc.M112.367987. Epub 2012 Jul 2.
  PUBMED    22761434
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.367987"; journalName: "J Biol Chem"; date: 
            "2012-08-17- 17"; volume: "287"; issue: "34"; pages: "28790-801"
COMMENT     Sequence Label: SFTPB Promoter reporter
FEATURES             Location/Qualifiers
     source          1..5195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    828..949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(839..858)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     893..912
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1197..1214)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1368..1956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1448..1467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2156..2947,2948..3016))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    3121..3169
                     /note="synthetic polyadenylation signal"
     misc_feature    3183..3274
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3223..3242
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     RBS             3526..3534
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      4321..4330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4327..5195,1..784)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca
       61 cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg
      121 cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct
      181 gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga
      241 ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct
      301 gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc
      361 tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga
      421 gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc
      481 cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc
      541 cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg
      601 taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa
      661 gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt
      721 ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt
      781 gtaataattc tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga
      841 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
      901 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
      961 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     1021 aaacctctac aaatgtggta aaatcgataa ggatccgtcg accgatgccc ttgagagcct
     1081 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     1141 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg
     1201 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1261 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1321 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1381 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1441 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1501 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1561 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1621 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1681 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1741 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1801 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1861 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1921 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1981 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2041 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2101 atatatgagt aaacttggtc tgacagcggc cgcaaatgct aaaccactgc agtggttacc
     2161 agtgcttgat cagtgaggca ccgatctcag cgatctgcct atttcgttcg tccatagtgg
     2221 cctgactccc cgtcgtgtag atcactacga ttcgtgaggg cttaccatca ggccccagcg
     2281 cagcaatgat gccgcgagag ccgcgttcac cggcccccga tttgtcagca atgaaccagc
     2341 cagcagggag ggccgagcga agaagtggtc ctgctacttt gtccgcctcc atccagtcta
     2401 tgagctgctg tcgtgatgct agagtaagaa gttcgccagt gagtagtttc cgaagagttg
     2461 tggccattgc tactggcatc gtggtatcac gctcgtcgtt cggtatggct tcgttcaact
     2521 ctggttccca gcggtcaagc cgggtcacat gatcacccat attatgaaga aatgcagtca
     2581 gctccttagg gcctccgatc gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg
     2641 taatggcagc actacacaat tctcttaccg tcatgccatc cgtaagatgc ttttccgtga
     2701 ccggcgagta ctcaaccaag tcgttttgtg agtagtgtat acggcgacca agctgctctt
     2761 gcccggcgtc tatacgggac aacaccgcgc cacatagcag tactttgaaa gtgctcatca
     2821 tcgggaatcg ttcttcgggg cggaaagact caaggatctt gccgctattg agatccagtt
     2881 cgatatagcc cactcttgca cccagttgat cttcagcatc ttttactttc accagcgttt
     2941 cggggtgtgc aaaaacaggc aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa
     3001 aatgttggat gctcatactc gtcctttttc aatattattg aagcatttat cagggttact
     3061 agtacgtctc tcaaggataa gtaagtaata ttaaggtacg ggaggtattg gacaggccgc
     3121 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
     3181 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
     3241 cccagtgcaa gtgcaggtgc cagaacattt ctctggccta actggccggt acctgagctc
     3301 ttctttctgc tgaaccatcg cagctatgcc ccagccccta ccctggaggg gtccccaggg
     3361 gccatgggca gcacctcctg tatagggctg tctgggagcc actccagggc cacagaaatc
     3421 ttgtctctga ctcagggtat tttgttttct gttttgtgta aatgctcttc tgactaatgc
     3481 aaaccatgtg tccatagaac cagaagattt ttccagggga aaaggtaagg aggtggtgag
     3541 agtgtcctgg gtctgccctt ccagggcttg ccctgggtta agagccaggc aggaagctct
     3601 caagagcatt gctcaagagt agagggggcc tgggaggccc agggagggga tgggagggga
     3661 acacccaggc tgcccccaac cagatgccct ccaccctcct caacctccct cccacggcct
     3721 ggagaggtgg gaccaggtat ggaggcttga gagcccctgg ttggaggaag ccacaagtcc
     3781 aggaacatgg gagtctgggc agggggcaaa ggaggcagga acaggccatc agccaggaca
     3841 ggtggtaagg caggcaggag tgttcctgct gggaaaaggt gggatcaagc acctggaggg
     3901 ctcttcagag caaagacaaa cactgaggtc gctgccactc ctacagagcc cccacgcccc
     3961 gcccagctat aaggggccat gccccaagca gggtacccag gctgcagagg tgccatggct
     4021 gagtcacacc tgctgcagtg gctgctgctg ctgctgccca cgctctgtgg cccaggcact
     4081 ggtgagtctc ccccagcctc ccctctccta ggcagctcca ccactcactg agcactgctt
     4141 tgtgctaggc attaacccaa gtctgtcctc attttaaaga caaggcagct ggggttcaga
     4201 gagggttcag agcttatcca aggtcacaca gctggcgggt ccaggagcag gtggaaccca
     4261 gagctgtctg acagatctgg cctcggcggc caagcttggc aatccggtac tgttggtaaa
     4321 gccaccatgg aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa
     4381 gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc
     4441 accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag
     4501 atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc
     4561 gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc
     4621 ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg
     4681 ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac
     4741 gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac
     4801 cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag
     4861 tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt
     4921 agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga
     4981 ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc
     5041 agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc
     5101 ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa
     5161 gactataaga ttcaatctgc cctgctggtg cccac
//
