LOCUS       sequence-178636-       10620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rosa26 targeting vector for intersectional Cre and FLP responsive 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Lusk SJ, McKinney A, Hunt PJ, Fahey PG, Patel J, Chang A, Sun JJ, 
            Martinez VK, Zhu PJ, Egbert JR, Allen G, Jiang X, Arenkiel BR, 
            Tolias AS, Costa-Mattioli M, Ray RS
  TITLE     A CRISPR toolbox for generating intersectional genetic mouse models 
            for functional, molecular, and anatomical circuit mapping.
  JOURNAL   BMC Biol. 2022 Jan 28;20(1):28. doi: 10.1186/s12915-022-01227-0.
  PUBMED    35086530
REFERENCE   2  (bases 1 to 10620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2022-01-28"; volume: "20(1):28. doi"; pages: " 
            10.1186/s12915-022-01227-0"
COMMENT     Sequence Label: 
            p15a_Kan_MLS_Rosa26_Short_CRISPRDel_FNeoStop_PStop_WPRE_bgh_pA
FEATURES             Location/Qualifiers
     source          1..10620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(132..149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(266..811)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(346..365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1984..2363
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2366..2641
                     /label=chicken beta-actin promoter
     intron          2642..3650
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3574..3596
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3658..3677
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3707..3740
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             3744..4547
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3807..3826)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4417..4436
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          5090..5155
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5285..5305
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5730..5864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    5906..5939
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    5946..5979
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          6519..6584
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6714..6734
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7159..7293
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    7335..7368
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7384..7972
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7437..7457)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7855..7866)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7988..8212
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    8224..9034
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     misc_feature    8548..9197
                     /label=HA-R
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     CDS             complement(9476..10291)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9476..10291)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10199..10218
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10283..10302
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct
       61 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata
      121 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
      181 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
      241 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
      301 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
      361 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
      421 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
      481 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta
      541 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca
      601 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac
      661 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt
      721 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca
      781 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata
      841 tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg
      901 atataagttg tatttcgtcg acggaagagc ggcgcgccaa atctccgttc tgtgagacag
      961 ccgggtacga gtcgtgacgc tggaaggggc aagcgggtgg tgggcaggaa tgcggtccgc
     1021 cctgcagcaa ccggaggggg agggagaagg gagcggaaaa gtctccaccg gacgcggcca
     1081 tggctcgggg gggggggggc agcggaggag cgcttccggc cgacgtctcg tcgctgattg
     1141 gcttcttttc ctcccgccgt gtgtgaaaac acaaatggcg tgttttggtt ggcgtaaggc
     1201 gcctgtcagt taacggcagc cggagtgcgc agccgccggc agcctcgctc tgcccactgg
     1261 gtggggcggg aggtaggtgg ggtgaggcga gctggacgtg cgggcgcggt cggcctctgg
     1321 cggggcgggg gaggggaggg agggtcagcg aaagtagctc gcgcgcgagc ggccgcccac
     1381 cctccccttc ctctggggga gtcgttttac ccgccgccgg ccgggcctcg tcgtctgatt
     1441 ggctctcggg gcccagaaaa ctggcccttg ccattggctc gtgttcgtgc aagttgagtc
     1501 catccgccgg ccagcggggg cggcgaggag gcgctcccag gttccggccc tcccctcggc
     1561 cccgcgccgc agagtctggc cgcgcgcccc tgcgcaacgt ggcaggaagc gcgcgctggg
     1621 ggcggggacg ggcagtaggg ctgagcggct gcggggcggg tgcaagcacg tttccgactt
     1681 gagttgcctc aagaggggcg tgctgagcca gacctccatc gcgcactccg gggagtggag
     1741 ggaaggagcg agggctcagt tgggctgttt tggaggcagg aagcacttgc tctcccaaag
     1801 tcgctctgag ttgttatcag taagggagct gcagtggagt aggcggggag aaggccgcac
     1861 ccttctccgg aggggggagg ggagtgttgc aatacctttc tgggagttct ctgctgcctc
     1921 ctggcttctg aggaccgccc tgggcctggg agaatccctt ccccctcttc tttctttaat
     1981 taagccattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     2041 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2101 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2161 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
     2221 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2281 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2341 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
     2401 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     2461 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
     2521 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     2581 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
     2641 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
     2701 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     2761 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
     2821 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
     2881 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
     2941 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     3001 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     3061 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     3121 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     3181 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     3241 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     3301 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     3361 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     3421 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     3481 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     3541 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     3601 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     3661 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgatgaag ttcctattct
     3721 ctagaaagta taggaacttc accatgggat cggccattga acaagatgga ttgcacgcag
     3781 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     3841 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3901 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     3961 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     4021 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     4081 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     4141 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     4201 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     4261 tgttcgccag gctcaaggcg cgcatgcccg acggcgatga tctcgtcgtg acccatggcg
     4321 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     4381 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     4441 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     4501 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctaatag tgagatatta
     4561 agggttccgg atcctcgggg acaccaaata tggcgatctc ggccttttcg tttcttggag
     4621 ctgggacatg tttgccatcg atccatctac caccagaacg gccgttagat ctgctgccac
     4681 cgttgtttcc accgaagaaa ccaccgttgc cgtaaccacc acgacggttg ttgctaaaga
     4741 agctgccacc gccacggcca ccgttgtagc cgccgttgtt gttattgtag ttgctcatgt
     4801 tatttctggc acttcttggt tttcctctta agtgaggagg aacataacca ttctcgttgt
     4861 tgtcgttgat gcttaaattt tgcacttgtt cgctcagttc agccataata tgaaatgctt
     4921 ttcttgttgt tcttacggaa taccacttgc cacctatcac cacaactaac tttttcccgt
     4981 tcctccatct cttttatatt ttttttctcg agggatcttt gtgaaggaac cttacttctg
     5041 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
     5101 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
     5161 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
     5221 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
     5281 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
     5341 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
     5401 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
     5461 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
     5521 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
     5581 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
     5641 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     5701 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     5761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5821 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgacat ggtaagtaag
     5881 cttgggctgc aggtcgaggg acctagaagt tcctattctc tagaaagtat aggaacttca
     5941 tcaggataac ttcgtataat gtatgctata cgaagttatg atatcattaa gggttccgga
     6001 tcctcgggga caccaaatat ggcgatctcg gccttttcgt ttcttggagc tgggacatgt
     6061 ttgccatcga tccatctacc accagaacgg ccgttagatc tgctgccacc gttgtttcca
     6121 ccgaagaaac caccgttgcc gtaaccacca cgacggttgt tgctaaagaa gctgccaccg
     6181 ccacggccac cgttgtagcc gccgttgttg ttattgtagt tgctcatgtt atttctggca
     6241 cttcttggtt ttcctcttaa gtgaggagga acataaccat tctcgttgtt gtcgttgatg
     6301 cttaaatttt gcacttgttc gctcagttca gccataatat gaaatgcttt tcttgttgtt
     6361 cttacggaat accacttgcc acctatcacc acaactaact ttttcccgtt cctccatctc
     6421 ttttatattt tttttctcga gggatctttg tgaaggaacc ttacttctgt ggtgtgacat
     6481 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
     6541 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
     6601 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
     6661 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
     6721 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
     6781 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
     6841 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
     6901 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
     6961 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
     7021 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
     7081 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     7141 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7201 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7261 aaactcatca atgtatctta tcatgtctgg atctgacatg gtaagtaagc ttgggctgca
     7321 ggtcgaggga cctaataact tcgtataatg tatgctatac gaagttatcc tgtttaaaca
     7381 tttaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7441 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7501 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7561 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7621 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7681 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7741 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     7801 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7861 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7921 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgaaata
     7981 attccgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     8041 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     8101 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     8161 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggttaattaa
     8221 agaagatggg cgggagtctt ctgggcaggc ttaaaggcta acctggtgtg tgggcgttgt
     8281 cctgcagggg aattgaacag gtgtaaaatt ggagggacaa gacttcccac agattttcgg
     8341 ttttgtcggg aagtttttta ataggggcaa ataaggaaaa tgggaggata ggtagtcatc
     8401 tggggtttta tgcagcaaaa ctacaggtta ttattgcttg tgatccgcct cggagtattt
     8461 tccatcgagg tagattaaag acatgctcac ccgagtttta tactctcctg cttgagatcc
     8521 ttactacagt atgaaattac agtgtcgcga gttagactat gtaagcagaa ttttaatcat
     8581 ttttaaagag cccagtactt catatccatt tctcccgctc cttctgcagc cttatcaaaa
     8641 ggtattttag aacactcatt ttagccccat tttcatttat tatactggct tatccaaccc
     8701 ctagacagag cattggcatt ttccctttcc tgatcttaga agtctgatga ctcatgaaac
     8761 cagacagatt agttacatac accacaaatc gaggctgtag ctggggcctc aacactgcag
     8821 ttcttttata actccttagt acactttttg ttgatccttt gccttgatcc ttaattttca
     8881 gtgtctatca cctctcccgt caggtggtgt tccacatttg ggcctattct cagtccaggg
     8941 agttttacaa caatagatgt attgagaatc caacctaaag cttaactttc cactcccatg
     9001 aatgcctctc tcctttttct ccatttataa actgagctat taaccattaa tggtttccag
     9061 gtggatgtct cctcccccaa tattacctga tgtatcttac atattgccag gctgatattt
     9121 taagacatta aaaggtatat ttcattattg agccacatgg tattgattac tgcttactaa
     9181 aattttgtca ttgtacacat ctgtaaaagg tggttccttt tggaatgcaa agttcaggtg
     9241 tttgttgtct ttcctgacct aaggtcttgt gagcttgtat tttttctatt taagcagtgc
     9301 tttctcttgg actggcttga ctcatggcat tctacacgtt attgctggtc taaatgtgat
     9361 tttgccaagc ttcttcagga cctataattt tgcttgactt gtagccaaac acaagtaaaa
     9421 tgattaagca acaaatgtat ttgtgaagct tggtccacca cgctcggagg gcctgttaga
     9481 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     9541 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     9601 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     9661 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     9721 ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat
     9781 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     9841 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     9901 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     9961 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
    10021 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
    10081 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
    10141 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
    10201 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg
    10261 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
    10321 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
    10381 tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
    10441 tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca
    10501 actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg
    10561 atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc
//
