LOCUS       sequence-178649-        8688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. 
            active mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Dagliyan O, Tarnawski M, Chu PH, Shirvanyants D, Schlichting I, 
            Dokholyan NV, Hahn KM
  TITLE     Engineering extrinsic disorder to control protein activity in living
            cells.
  JOURNAL   Science. 2016 Dec 16;354(6318):1441-1444.
  PUBMED    27980211
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-12-16"; volume: "354(6318)"; pages: "1441-1444"
COMMENT     Sequence Label: pBabe-PI(WT)-Src(YF)-mCherry
FEATURES             Location/Qualifiers
     source          1..8688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(28..527)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(76..95)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     469..492
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    560..576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(590..1300)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(868..887)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1137..1155
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(2057..2485)
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
     regulatory      3358..3367
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3459..3483)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3480..3518)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(3551..3569,3570..3592,3593..3611,3612..3634,3635..3653,
                     3654..3676,3677..3695,3696..3718,3719..3737,3738..3760,
                     3761..3779,3780..3802,3803..3821)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     CDS             complement(3862..4278)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(3878..3894)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3910..3932)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    4479..4678
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             4743..5212
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        5243..5347
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5348..5416,5417..6208)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5566..5585)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6368..6956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6857..6876
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7110..7127
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(7604..8629)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7604..8629)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 cagctggcaa gcttattggc tgcaggtcga aaggcccgga gatgaggaag aggagaacag
       61 cgcggcagac gtgcgctttt gaagcgtgca gaatgccggg cctccggagg accttcgggc
      121 gcccgccccg cccctgagcc cgcccctgag cccgcccccg gacccacccc ttcccagcct
      181 ctgagcccag aaagcgaagg agcaaagctg ctattggccg ctgccccaaa ggcctacccg
      241 cttccattgc tcagcggtgc tgtccatctg cacgagacta gtgagacgtg ctacttccat
      301 ttgtcacgtc ctgcacgacg cgagctgcgg ggcggggggg aacttcctga ctaggggagg
      361 agtagaaggt ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc taccggtgga
      421 tgtggaatgt gtgcgaggcc agaggccact tgtgtagcgc caagtgccca gcggggctgc
      481 taaagcgcat gctccagact gccttgggaa aagcgcctcc cctacccggt agaattgacc
      541 tgcaggggcc ctcgacaaat tgttatccgc tcacaattcc acactcgact tacttgtaca
      601 gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg
      661 tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca
      721 gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt
      781 ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc
      841 gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga
      901 agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct
      961 gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct
     1021 cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg
     1081 agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccacccttgg
     1141 tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga
     1201 tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactcct
     1261 tgatgatggc catgttatcc tcctcgccct tgctcaccat tcctggtccg cctaggttct
     1321 ccccgggctg gaactgtggc tcagtggacg taaagtagtc ttccaggaag gcctgcaggt
     1381 actcgaaggt gggccgctcc tcgggctcct tccgccagca ctggcacata aggtcatgca
     1441 gggactcggg gcactcgggg ggacaaggca tccggtagcc ccgctccacc tggtccagaa
     1501 cctcacggtt caccatccca ggatagggca ctcttccctt agtggtgagc tcggtcagca
     1561 gaatcccaaa ggaccacaca tccgacttga tggtgaacct gccgtacaga gcagcttcag
     1621 gggcggtcca cttgatgggg aatttggcac cttgccgggc tgtgtattcg ttgtcttcta
     1681 tgagccgggc caacccaaag tcggccactt tgcacaccag gttctcccct actaggatat
     1741 tggcggctcg aaggtcccgg tgcacatagt tcatccgctc cacataggcc atgcctgaag
     1801 cgatctgagc agacatgtcc accagctggg gtagccgcaa atatttgccc gtttccccct
     1861 tgagaaagtc cagcagactc cccttgttca tgtactctgt cacaatgtaa atgggttctt
     1921 ccgacaccac agcatacagc tgcaccagtt tctcgtgcct cagtttcttc atgacttggg
     1981 cctcctgcag gaaggcctct ggggacatgg tgcctggctt cagagttttg atggcaaccc
     2041 tcgtggtgcc tggtccaagt tcttttgccg cctcatcaat attttctgca gttttcttaa
     2101 tcagcatgac tccctctctc tcggcagcat ctcggacatg ctcagttcca tccaactgaa
     2161 ccccaataaa gtactggaca tctcccttct gatctcgcat aggctgcaag tgaaagaggt
     2221 tccagaactt tttaccactc tttgtataat taatcagctg aacagtgacc tctgtttggt
     2281 tatctatggc atctctaatt tttctcactg tcgcgcgatc agtttcagga ccttgtagaa
     2341 acctgcagtt tcttcccaaa atttcttcac ggctatattc tgtcaactgc aagaaactat
     2401 cggacgcgaa tataatggga ttatctggca atcttgggtc agtaatgaca aagttcttct
     2461 caatacgttc aagtgtagta gccaagcctg gtccgttcca ggtccccatc cacacctctc
     2521 cgaagcaacc ctggcccagc ttgacctcca gccgcaggga ctcccggggg atctcccacg
     2581 catccttggc caatccctgg gtctgaggct tggatgtggg acatacggta gtgaggcggt
     2641 gacacaggcc atcagcatgt ttggagtagt aagccacgag ctgctgcagg ctgttgaact
     2701 gggtgcggga ggtgatgtag aaaccgccgc tgtccagctt gcggatcttg tagtgtttca
     2761 catttaggcc cttggcattg tcgaagtcgg atacagagag gcagtaggca ccttttgtgg
     2821 tctcactctc cctcacgagg aaggtccctc tcgggttctc ggcgttgagc agcagccgct
     2881 ctgattcccg tctagtgatc ttgccaaagt accactcctc agcctggatg gagtcggagg
     2941 gcgccacata gttgctgggg atgtaaccgg tctgtcccgt gctcagcgag tgtgccagcc
     3001 accagtctcc ctccgtgttg ttaacaatct gcagccgctc ccctttcttg aaggacaggt
     3061 cagtctctgt ccgtgactca tagtcataga gggccacaaa ggtggtcacc ccacctgcca
     3121 gaggccccgc cctctgcggg gaggtgacgg tgtccgagga gttgaagcct ccgaagagct
     3181 tgggctcggc cgcgggcggc acgaaggcgg cgctgggccc gcggtggccg tcggcggagg
     3241 cgggcttgct cggtgtctgt gaggccggga aggcgccccc tgccccgtgc acgttttccg
     3301 agggctccag gctgcggcgc cgctggctgg cgtccttggg cttgctcttg ttgctgccca
     3361 tggtggcgaa ttcactttac ggatctgggg ccgcggaggc tggatcggtc ccggtgtctt
     3421 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga
     3481 gctctgctta tataggcctc ccaccgtaca cgcctacctc gacccgggta ccgagctcga
     3541 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
     3601 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
     3661 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
     3721 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
     3781 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgacgacga
     3841 aagggcctcg tggatccggc cggcgcctag agaaggagtg agggctggat aaagggagga
     3901 tcgaggcggg gtcgaacgag gaggttcaag ggggagagac ggggcggatg gaggaagagg
     3961 aggcggaggc ttagggtgta caaagggctt gacccaggga ggggggtcaa aagccaaggc
     4021 ttcccaggtc acgatgtagg ggacctggtc tgggtgtcca tgcgggccag gtgaaaagac
     4081 cttgatctta acctgggtga tgaggtctcg gttaaaggtg ccgtctcgcg gccatccgac
     4141 gttaaaggtt ggccattctg cagagcagaa ggtaacccaa cgtctcttct tgacatctac
     4201 cgactggttg tgagcgatcc gctcgacatc tttccagtga cctaaggtca aacttaaggg
     4261 agtggtaaca gtctggccct aattttcaga caaatacaga aacacagtca gacagagaca
     4321 acacagaacg atgctgcagc gctgcagcag acaagacgcg cggcttcggt tccaaaccga
     4381 aagcaaaaat tcagacggag gcgggaactg ttttaggttc tcgtctccta ccagaaccac
     4441 atatcctgac ggggtcggat tccacatcga ctcccttcct caggtcgggc cacaaaaacg
     4501 gcccccaaag tccctgggac gtctcccagg gttgcggccg ggtgttcaga actcgtcagt
     4561 tccaccacgg gtccgccaga tacagagcta gttagctaac tagtaccgac gcaggcgcat
     4621 aaaatcagtc atagacacta gacaatcgga cagacacaga taagttgctg gccagcttgc
     4681 ctcccggtgg tgggtcggtg gtccctgggc aggggtctcc cgatcccgga cgagccccca
     4741 aatgaaagac ccccgctgac gggtagtcaa tcactcagag gagaccctcc caaggaacag
     4801 cgagaccaca agtcggatgc aactgcaaga gggtttattg gatacacggg tacccgggcg
     4861 actcagtcaa tcggaggact ggcgccccga gtgaggggtt gtgggctctt ttattgagct
     4921 cggggagcag aagcgcgcga acagaagcga gaagcgaact gattggttag ttcaaataag
     4981 gcacagggtc atttcaggtc cttggggcac cctggaaaca tctgatggtt ctctagaaac
     5041 tgctgagggc tggaccgcat ctggggacca tctgttcttg gccctgagcc ggggcaggaa
     5101 ctgcttacca cagatatcct gtttggccca tattcagctg ttccatctgt tcttggccct
     5161 gagccggggc aggaactgct taccacagat atcctgtttg gcccatattc aggctgcagg
     5221 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5281 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5341 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5401 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5461 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5521 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5581 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5641 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5701 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5761 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5821 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5881 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5941 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6001 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6061 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6121 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6181 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6241 gattgatttg cggccgcaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6301 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6361 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6421 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6481 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6541 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6601 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6661 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6721 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6781 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6841 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6901 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6961 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7021 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7081 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7141 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc aactatggcc
     7201 atttaatgta aatacttaag aaaaaaaacc aaattaattt tgatacatgc tgcatgtgaa
     7261 agacccccgc tgacgggtag tcaatcactc agaggagacc ctcccaagga acagcgagac
     7321 cacaagtcgg atgcaactgc aagagggttt attggataca cgggtacccg ggcgactcag
     7381 tcaatcggag gactggcgcc ccgagtgagg ggttgtgggc tcagggatct cctaccttga
     7441 tctgaacttc tctattctca gttatgtatt tttccatgcc ttgcaaaatg gcgttactta
     7501 agctagcttg ccaaacctac aggtggggtc tttcattccc ccctttttct ggagactaaa
     7561 taaaatcttt tattttatcg atagatcccg gtcggcatct actctattcc tttgccctcg
     7621 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     7681 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     7741 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     7801 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
     7861 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     7921 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     7981 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     8041 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     8101 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
     8161 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
     8221 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
     8281 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
     8341 cctgtgcacg gcgggagatg caataggtca ggctctcgct aaattcccca atgtcaagca
     8401 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
     8461 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
     8521 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
     8581 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc
     8641 ccccgggatc agatctgcag ggcttcccaa ccttaccaga gggcgccc
//
