LOCUS       sequence-178651-        2725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express csgA curli protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdelwahab MT, Kalyoncu E, Onur T, Baykara MZ, Seker UO
  TITLE     Genetically-Tunable Mechanical Properties of Bacterial Functional 
            Amyloid Nanofibers.
  JOURNAL   Langmuir. 2017 Apr 7. doi: 10.1021/acs.langmuir.7b00112.
  PUBMED    28388843
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Langmuir. 
            2017 Apr 7. doi: 10.1021/acs.langmuir.7b00112."
COMMENT     Sequence Label: pZa-tetO-csgA-CmR
FEATURES             Location/Qualifiers
     source          1..2725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             466..477
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(512..537,538..543,544..560,561..566,
                     567..585))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    542..560
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    567..585
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    838..856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    863..881
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        920..1022
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1023..1682
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1089..1108)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1703..1797
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1911..2456
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2357..2376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2573..2590
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2625..2711)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgtttagtac tgatgagcgg tcgcgttgtt accaaagcca acctgagtca cgttgacgga
       61 ggagttagat gcagtctggt caactgcagc accgttgcca ccaccgaact gtttaaccgt
      121 catttcagaa tttttgccgt tccactgatc aagagtagcg ctgttaccga agccacgttg
      181 ggtcagatcg attgagctgt catctgagcc ctgaccaaca tctgcaccat taccgccgcc
      241 atgctgggta atagtcaagt cagagttacg ggcatcagtt tgcagagcaa gtgcagagtt
      301 accgccaccg tactggtaaa tgttcagctc agaatttggg ccgctattat taccgccacc
      361 accgtggtta ccgccgccgc cgtactgagg aacaacacct gccagagcgc taccggagaa
      421 tacgattgct gcaattgctg ctacttttaa aagtttcatg gtacctttct cctctttaat
      481 acccaaatcc aagaggtgaa tggtagaatt cggtcagtgc gtcctgctga tgtgctcagt
      541 atctctatca ctgataggga tgtcaatctc tatcactgat agggactcga gaaggcccag
      601 tctttcgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcatgggg
      661 agaccccaca ctaccatcgg cgctacggcg tttcacttct gagttcggca tggggtcagg
      721 tgggaccacc gcgctactgc cgccaggcat ctagagatat atggtagtag taagttaatt
      781 ttcattaacc accactacca atcacctcct ggatttgggt gtcgactgtg ctcagtatct
      841 ctatcactga tagggatgtc aatctctatc actgataggg agggcccttt gacgtcgata
      901 tctggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa
      961 taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta
     1021 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     1081 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     1141 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     1201 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
     1261 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     1321 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     1381 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     1441 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     1501 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     1561 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     1621 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     1681 aatttgatat cgagctcgct tggactcctg ttgatagatc cagtaatgac ctcagaactc
     1741 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca
     1801 agcactagta acaacttata tcgtatgggg ctgacttcag gtgctacatt tgaagagata
     1861 aattgcactg aaatctagaa atattttatc tgattaataa gatgatcttc ttgagatcgt
     1921 tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt
     1981 tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg aggagcgcag
     2041 tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa gactaactcc
     2101 tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt ccgggttgga
     2161 ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat
     2221 acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca
     2281 aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca
     2341 cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2401 actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg
     2461 gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt ccaggaaatc
     2521 tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc
     2581 agtgagcgag gaagcggaat atatccctag gtctagggcg gcggatttgt cctactcagg
     2641 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt
     2701 cgttttattt gatgcctcta gcacg
//
