LOCUS       sequence-178689-         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA
            containing a shorter version of the wt spacer (-12) truncated by 12 
            nucleotides at the leader-distal end..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Kuznedelov K, Mekler V, Lemak S, Tokmina-Lukaszewska M, Datsenko KA,
            Jain I, Savitskaya E, Mallon J, Shmakov S, Bothner B, Bailey S, 
            Yakunin AF, Severinov K, Semenova E
  TITLE     Altered stoichiometry Escherichia coli Cascade complexes with 
            shortened CRISPR RNA spacers are capable of interference and primed 
            adaptation.
  JOURNAL   Nucleic Acids Res. 2016 Dec 15;44(22):10849-10861. Epub 2016 Oct 13.
  PUBMED    27738137
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-12-15"; volume: "44(22)"; pages: 
            "10849-10861. Epub 2016 Oct 13"
COMMENT     Sequence Label: pCDF-casBCDE(-12)
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtcttgatgg gtttgaaaat gggagctcgg tttatccccg ctggcgcggg gaactcccct
       61 cagcagcgaa agacagcggt ttatccccgc tggcgcgggg aactctctaa aagtatacat
      121 ttgttcttaa agcatttttt cccataaaaa caacccacca accttaaacc atccaaatct
      181 cgagtgcggc cgctcacagt ggagccaaag atagcaagcc acatcccatc gatttagctg
      241 gcccaatacc ttgctgtaca agatctatta acgctggcgc gtcgttgatg gtgagcacac
      301 cttcaaagca aaccgtttgg atctttccac ttttaccatc accagaaaaa tactgtggcc
      361 gttccgatat gggatgcaca tcttcaacgc gcgccgcatt gcccaattta cgttgcaacc
      421 acgcgatttg ttctgcttct tttattaacg gaacccgaca gcgtttaata ttccctttac
      481 tgtccaggcg cttttgattg tcgagaatag ttttgatcgg atttgcccga agccgaaaat
      541 agagtggaac accaacctga agttgaaatt caacctgttt agttttaatg actgtcgcaa
      601 cggcagttga aacaggcatt tgcgctgact gcaataaaac atgacagcct tctggtgtgt
      661 ttcgcttctc aacatgaaaa agaaaatcac gagcagcatc cggtctgttt
//
