LOCUS       sequence-178723-        2660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a HR repair template for knock-in a YFP expression cassette 
            downstream of the poly(A) signal of human NEAT1_1 isoform. Best used
            with px330-NEAT1m_v1 vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Li R, Harvey AR, Hodgetts SI, Fox AH
  TITLE     Functional dissection of NEAT1 using genome editing reveals 
            substantial localisation of the NEAT1_1 isoform outside 
            paraspeckles.
  JOURNAL   RNA. 2017 Mar 21. pii: rna.059477.116. doi: 10.1261/rna.059477.116.
  PUBMED    28325845
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Mar 21. pii: rna.059477.116. doi: 10.1261/rna.059477.116."
COMMENT     Sequence Label: pYFP-NEAT1m_v1-RT
FEATURES             Location/Qualifiers
     source          1..2660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        541..844
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        845..1048
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     999..1019
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1087..1096
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1093..1095,1096..1098,1099..1809)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1138..1159)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1399..1418)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1746..1767
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1810..1875
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1999..2120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2036..2055)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2090..2109
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gttctgagct gcgtctattg aattggtaaa gtaataccaa tggcttttta tcatttcctt
       61 cttcccttta agtttcactt gaaattttaa aaatcatggt tatttttatc gttgggatct
      121 ttctgtcttc tgggttccat tttttaaatg tttaaaaata tgttgacatg gtagttcagt
      181 tcttaaccaa tgacttgggg atgatgcaaa caattactgt cgttgggatt tagagtgtat
      241 tagtcacgca tgtatgggga agtagtctcg ggtatgctgt tgtgaaattg aaactgtaaa
      301 agtagatggt tgaaagtact ggtatgttgc tctgtatggt aagaactaat tctgttacgt
      361 catgtacata attactaatc acttttcttc ccctttacag cacaaataaa gtttgagttc
      421 taaactcatt agaattgttg tattgctatg ttacatttct cgacccctat cacattgcct
      481 tcataacgaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      541 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      601 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      661 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      721 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      781 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      841 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      901 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      961 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     1021 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
     1081 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1141 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     1201 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1261 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc
     1321 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1381 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1441 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1501 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1561 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1621 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1681 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     1741 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1801 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1861 gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     1921 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     1981 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2041 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2101 aaactcatca atgtatctta actttggatg tatcttcata ttgtagattt aggtctagat
     2161 ttgctagctc caagtaatta aggccatgta ggagagcatg gtaaccacag atagaactgg
     2221 tattatccca agtggtctgc agactgctga gtggggatgg gatctgctct ctgttgagag
     2281 ttggtaatca ttggtttgaa atgtgatgaa accactcaag ccaatgaagg tgggtgtgta
     2341 ggtggggagt actttgccat aatattttaa aacattacct ggttagagtt ctaagtggta
     2401 cttatttttg tttggttagg ggaaagcctg aataaaaaca gaaatggaca cataatatgc
     2461 atattccata gtctttggga ggctggaatg tgcctgggat ttgggtctaa gtgtatgcgt
     2521 aattcttacc tcactaaaga atttgccttg tttttttcct tttggtgagt gactaaaacg
     2581 tctgggcttc cctgtgtgcg tgctacagta agcaagcaga ggctgtgcaa aggtgtgagc
     2641 aggatcacgt ggaatctgga
//
