LOCUS       sequence-178727-         492 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a HR repair template that mutates the poly(A) signal as well
            as a few splicing factor binding sites of human NEAT1_1 isoform. 
            Best used with px335-NEAT1_IS_v1 and v2 vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Li R, Harvey AR, Hodgetts SI, Fox AH
  TITLE     Functional dissection of NEAT1 using genome editing reveals 
            substantial localisation of the NEAT1_1 isoform outside 
            paraspeckles.
  JOURNAL   RNA. 2017 Mar 21. pii: rna.059477.116. doi: 10.1261/rna.059477.116.
  PUBMED    28325845
REFERENCE   2  (bases 1 to 492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Mar 21. pii: rna.059477.116. doi: 10.1261/rna.059477.116."
COMMENT     Sequence Label: pYFP-NEAT1_IS_RT
FEATURES             Location/Qualifiers
     source          1..492
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttaaaaatca tggttatttt tatcgttggg atctttctgt cttctgggtt ccatttttta
       61 aatgtttaaa aatatgttga catggtagtt cagttcttaa ccaatgactt ggggatgatg
      121 caaacaatta ctgtcgttgg gatttagagt gtattagtca cgcatgtatg gttaagtagt
      181 ctcgggtatg ctgttgtgaa attgaaactc gaaaagtaga tggttgaaag tactggtatg
      241 ttgctctcga tggtaagaac taattctgtt acgtcatcga cataattact aatcactttt
      301 cttccccttg ctttacagca cagacaaggt ttgagttcta aactcattag aattgttgta
      361 ttgctatgtt acatttctcg acccctatca cattgccttc ataacgactt tggatgtatc
      421 ttcatattgt agatttaggt ctagatttgc tagctccaag taattaaggc catgtaggag
      481 agcatggtaa cc
//
