LOCUS       sequence-178739-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP in the presence of Pseudomonas fluorescence sigma 
            factor ECF20_992. Repressed by TetR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  TITLE     Unpublished Murray Lab Plasmids
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P20_992-tetO-deGFP
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(31..52)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(292..311)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     639..660
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggagcttt tcactggcgt tgttcccatc ctggtcgagc tggacggcga cgtaaacggc
       61 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      121 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      181 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      241 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      301 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      361 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      421 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
      481 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      541 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
      601 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      661 accgccgccg ggatctaa
//
