LOCUS       sequence-178747-         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TetR in the presence of ipgC and mxiE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  TITLE     Unpublished Murray Lab Plasmids
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PipaH-TetR
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..621
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(71..90)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 tctagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga
       61 atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg
      121 tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat
      181 aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat
      241 aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta
      301 ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc
      361 caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact
      421 ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct
      481 actactgata gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt
      541 gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt
      601 aaatgtgaaa gtgggtctta a
//
