LOCUS       sequence-178750-         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with 
            RFP657 expression (EFS Promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Reimer J, Knoess S, Labuhn M, Charpentier EM, Gohring G, 
            Schlegelberger B, Klusmann JH, Heckl D
  TITLE     CRISPR-Cas9-induced t(11;19)/MLL-ENL translocations initiate 
            leukemia in human hematopoietic progenitor cells in vivo.
  JOURNAL   Haematologica. 2017 Jun 1. pii: haematol.2017.164046. doi: 
            10.3324/haematol.2017.164046.
  PUBMED    28572162
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Haematologica. 2017 Jun 1. pii: haematol.2017.164046. doi: 
            10.3324/haematol.2017.164046."
COMMENT     Sequence Label: SGL40C.EFS.RFP657
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(285..533)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(343..362)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(513..533)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ggcggagcca gtacacgaca tcactttccc agtttacccc gcgccacctt ctctaggcac
       61 cggttcaatt gccgacccct ccccccaact tctcggggac tgtgggcgat gtgcgctctg
      121 cccactgacg ggcaccggag ccactcgagt tctagaggag aattcaaaaa agcaccgact
      181 cggtgccact ttttcaagtt gataacggac tagccttatt taaacttgct atgctgtttc
      241 cagcatagct cttaaacaga gacgagttgt cgacatacgt ctccggtgtt tcgtcctttc
      301 cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag catatgatag
      361 tccattttaa aacataattt taaaactgca aactacccaa gaaattatta ctttctacgt
      421 cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattctaat tatctctcta
      481 acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc ctcggtacct
      541 taattaacca aactggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
      601 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
      661 cccctgcact gtacccccc
//
