LOCUS       sequence-178821-        8041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains dusp5 enhancer sequence, and dusp5 specific gene promoter 
            driving expression of eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND
  TITLE     Robust Identification of Developmentally Active Endothelial 
            Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq.
  JOURNAL   Cell Rep. 2017 Jul 18;20(3):709-720. doi: 
            10.1016/j.celrep.2017.06.070.
  PUBMED    28723572
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-07-18- 18"; volume: "20"; issue: "3"; pages: "709-720"
COMMENT     Sequence Label: pT2-cryR;dusp5CE1-P1Egfp
FEATURES             Location/Qualifiers
     source          1..8041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     638..654
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    674..694
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1457..1481
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2058..2067
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2064..2066,2067..2069,2070..2780)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2064..2066,2067..2069,2070..2780)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2109..2130)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2370..2389)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2717..2738
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2781..2846
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(2867..2891)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2917..2937)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2945..2961)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2977..3111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(3550..3737,3738..3749)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3783..3799)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3783..3799)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3836..3856)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3836..3854)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3875..3891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3888..3910)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3899..3915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3923..3929,3930..3947,3948..3953))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3968..3989
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4106..4123)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4277..4865)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4357..4376)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5036..5827,5828..5896))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5659..5678
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5897..6001)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6164..6185)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(6534..6545,6546..6708)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    7077..7198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7321..8031)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(7401..7424)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(7599..7618)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7868..7886
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      8028..8037
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtaatgtca gacctggtaa ctccttactg taacttattg cacctgatga acaaaagatg
       61 tgaactgaac gcaagtagga ggctaataga ggctagtaca gtacaaagat ccccaaatga
      121 tgtgtgccgg ccttagtgga ggcagacccc tggttatata cctgtgctgc agaaagaggg
      181 gcttacaaat ggatctctct ggaatggcat tatctcatgg tgggaacagt gttgtcagca
      241 gaatcagcgg ggtggactct gacctgagaa tgacagtcta gcactgaatg gctcagacaa
      301 tactcttgtg gccaactatc accggaaaat aagtgcctgc caaaacaaac cagaccagat
      361 gaagctacat ggctgtagaa cgtcagtatt taatgtgttg tggccacttg gttcaccaga
      421 tttaagagct aattttttta aatatcacag ataatcaatc gctttttttt ttcttgactg
      481 aaaagcttag actggaaaat tctttgaaaa agtttgcact acagaaaata aatacataac
      541 atcctgcact gaatgcacac tattaataga aaagagagca ttattgtagg aaatgtattg
      601 tatatgtaca ttctgtgcat ttcacaattt tctcgagcag gaaacagcta tgaccatgat
      661 tacgccaagc tatcaacttt gtatagaaaa gttggttaaa acaactacac tttcataaag
      721 gtcaacatct acatatctac aagcattaca ttttttgttt tcaactgctg tataagcaac
      781 atgtgctaac atgtaatagg cggcaagaca gctgagtaaa atctgcaaaa cacacacaca
      841 cacacacact catgcacttg agcagcagat gaagatctgt ctgtggttca ttagtaatga
      901 ggctctcgca gacacacctc atctccaccc tgctgctgtg gaactgggag ttttacgcca
      961 caccacagtt tcattctcac tctaaatgga aactgtgcgt gcactcccca atttaagcac
     1021 acaacctaat ttttcaaaag atggccacaa agtttgagct gaccagttgg atgttgtgtt
     1081 gtttttgctg agtcctcgag gtatgggatg tttacatgcc ctgaggatgt gcattgtttc
     1141 tcatgcctgg tcttgtagct gccagtctag atctgctgag agctgttccc atttcctgct
     1201 ttacaggaag tgtcggcccc cactggtctt tgtcactcag agagacagga agaggaaacg
     1261 gcccacagga aacatctccc ccacttgcta cgaatcatcc cgcaacatga gcctacaagc
     1321 acaaactgtt ttcatttagt tagtttatgg tagaacacac tgtgaaagtg aagatgtttg
     1381 caatggtctg acctaaatgc ttttatttgt gtgacacaat gtaattagga cagttaatgc
     1441 atgattaagt agtcgaacaa gtttgtacaa aaaagcaggc tcgctattca gtcaaatttt
     1501 gccaacaact tgtttttatg aaatacaaag gaccagattc agctttaatt ttcttactga
     1561 agaaaaaagt tgtttatata tgatacatta aaagcatatt tttgtttttg aggtatttct
     1621 tttaagtgct tgaactttta aagtgacaaa ctgaagtacc tcatcatcct ttgttggtgt
     1681 gggcgataaa atagcctagt tgtcctaaaa taaccttcta tgttgtcgtt ttcaaatact
     1741 ttcaaggtta ttacaataaa tgagcgcttg aattacaaga aagtgacaca tatgagcagc
     1801 accgtgatga accgatgagt taaatgttca gtatttattc gcctcactcg gttttcaagg
     1861 gcgggttcag tcaacagaat gtgggcgcgc tcccgtgtcg gtgtgtccat atatggttcg
     1921 tgacgtcaag cgccggaggt tccattcaca taaaaccgat cagacggaat ccccttggag
     1981 gaaatgtaga ctcaggctca gtacgcaggg tttagtaggc aacctacacc cctgaaacat
     2041 ccacatccct atttatcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     2101 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2161 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2221 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     2281 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2341 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2401 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2461 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2521 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     2581 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2641 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     2701 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2761 gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg aattctgcag
     2821 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgataccc agctttcttg
     2881 tacaaagtgg ggcgcgccta cgtaactagt ctcgagcaac tttattatac atagttgata
     2941 attcactggc cgtcgtttta cggtaccatc gatgatgatc cagacatgat aagatacatt
     3001 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     3061 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     3121 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     3181 taaaacctct acaaatgtgg tatggctgat tatgatcctc tagatcagat ctgcgaagat
     3241 acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc gcagctgctg
     3301 atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt tccctaattt
     3361 ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact tccaaaggac
     3421 caatgaacat gtctgaccaa tttcatataa tgtgaaaacg attttcatag gcagaataaa
     3481 taacatttaa attaaactgg gcatcagcgc aattcaattg gtttggtaat agcaagggaa
     3541 aatagaatga agtgatctcc aaaaaataag tactttttga ctgtaaataa aattgtaagg
     3601 agtaaaaagt actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt
     3661 ttaattgtac tcaagtaaag taaaaatccc caaaaataat acttaagtac agtaatcaag
     3721 taaaattact caagtacttt acacctctgg ttcttgaccc cctaccttca gcaagcccag
     3781 cagatccact agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
     3841 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     3901 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3961 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     4021 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4081 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4141 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4201 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4261 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4321 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4381 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4441 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4501 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4561 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4621 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4681 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4741 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4801 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4861 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4921 gttaaggcat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4981 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5041 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5101 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5161 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5221 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5281 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5341 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5401 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5461 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5521 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5581 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5641 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5701 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5761 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5821 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5881 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5941 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6001 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     6061 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     6121 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     6181 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     6241 tggcccacgc tggctaagaa ctcatcagcc tccccggtcc atctacccac gtaccaatgc
     6301 accaattggc cacaatgacg gctactacat ggtgccattc cttcctcttt ataggaatgg
     6361 agactacctc ctgtccaaca atgctcttgg atacgagtac gcctacctgt tggacccagg
     6421 tcattgcaca acaccagaaa tgccctctga tctgcaaaag acgtgaatat ctgttcagac
     6481 acccatatcc actctgttcc acacaggtca gaggtttgtc caggagttct tgacagaggt
     6541 gtaaaaagta ctcaaaaatt ttactcaagt gaaagtacaa gtacttaggg aaaattttac
     6601 tcaattaaaa gtaaaagtat ctggctagaa tcttacttga gtaaaagtaa aaaagtactc
     6661 cattaaaatt gtacttgagt attaaggaag taaaagtaaa agcaagaaag aaaactagag
     6721 attcttgttt aagcttttaa tctcaaaaaa cattaaatga aatgcataca aggttttatc
     6781 ctgctttaga actgtttgta tttaattatc aaactataag acagacaatc taatgccagt
     6841 acacgctact caaagttgta aaacctcaga tttaacttca gtagaagctg attctcaaaa
     6901 ttgttagtgt caagcctagc tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc
     6961 ctctcacagg cagccgatgc gggaagaggt gtattagtct tgatagagag gctgcaaata
     7021 gcaggaaacg tgagcagaga ctccctggtg tctgaaacac aggcccattt acgccttaag
     7081 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     7141 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     7201 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     7261 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt cgcggccgct
     7321 ttacttgtac agctcgtcca tgccgccggt ggagtggtgg ccctcggcgc gctcgtactg
     7381 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc agcttgatgt tgacgttgta
     7441 ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cggcgtcgta
     7501 gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc
     7561 ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc
     7621 gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag
     7681 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca
     7741 cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac
     7801 gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg
     7861 gccgcccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc
     7921 ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg
     7981 catgaactcc ttgatgatgg ccatgttgtc atcctcgccc ttgctcacca tggtggcgac
     8041 c
//
