LOCUS       sequence-178823-        5183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B108 enhancer of Blimp1 gene drives IRES-Cre expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Wang S, Sengel C, Emerson MM, Cepko CL
  TITLE     A gene regulatory network controls the binary fate decision of rod 
            and bipolar cells in the vertebrate retina.
  JOURNAL   Dev Cell. 2014 Sep 8;30(5):513-27. doi: 
            10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
  PUBMED    25155555
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.07.018"; journalName: "Dev Cell"; date: 
            "2014-09-8- 8"; volume: "30"; issue: "5"; pages: "513-27"
COMMENT     Sequence Label: B108-Cre2
FEATURES             Location/Qualifiers
     source          1..5183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    348..439
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(380..399)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    453..501
                     /note="synthetic polyadenylation signal"
     promoter        573..677
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(678..746,747..1538)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(896..915)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1709..2297
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2198..2217
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2451..2468
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    2536..2670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2560..2579)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2614..2633
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(2676..2871)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2690..2825
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2930..2951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2966..2971,2972..2989,2990..2996)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3004..3020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3009..3031
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3028..3044
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3028..3044
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3387..3406
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    3405..3460
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     3506..3525
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(3621..3650)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(3660..4688)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     misc_feature    join(4689..4697,4698..4700,4701..5183,1..92)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4862..4881)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5091..5108
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 atatggtgga aaataacata tagacaaacg cacaccggcc ttattccaag cggcttcggc
       61 cagtaacgtt aggggcgggg gagggagagg gggggggggg gcggaattta cgtagcggcc
      121 gcggtggcga ccggtgcgcg cataaagcgc ctcgcaaatc gcgtccgacg ggcaactaac
      181 ggctccggtc aagcttccat tatatagaat tcaattaaag ctgataaatc caagctactt
      241 gctgcaggtc taattcctcc taattgtatt cagggatgaa gcaagcttgt ctaatctccc
      301 aagtacctga cctatttccg aactcgagca attggtcgac tatcgataga gaaatgttct
      361 ggcacctgca cttgcactgg ggacagccta ttttgctagt ttgttttgtt tcgttttgtt
      421 ttgatggaga gcgtatgtta gtactatcga ttcacacaaa aaaccaacac acagatgtaa
      481 tgaaaataaa gatattttat tgcggcgctc caagtacctc ccgtacctta atattacttc
      541 tcgacccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      601 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      661 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      721 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      781 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      841 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      901 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
      961 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1021 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1081 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1141 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1201 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1261 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1321 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     1381 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     1441 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     1501 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     1561 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1621 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1681 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1741 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1801 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1861 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1921 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1981 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2041 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2101 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2161 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2221 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2281 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2341 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2401 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2461 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2521 ttcattaatg cagcggatcc agacatgata agatacattg atgagtttgg acaaaccaca
     2581 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     2641 gtaaccatta taagctgcaa taaacaagtt agctttttgc aaaagcctag gcctccaaaa
     2701 aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa
     2761 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2821 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg cggatccgct
     2881 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     2941 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     3001 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     3061 ttgggctgca ggtcgaggga tctccataag agaagaggga cagctatgac tgggagtagt
     3121 caggagagga ggaaaaatct ggctagtaaa acatgtaagg aaaattttag ggatgttaaa
     3181 gaaaaaaata acacaaaaca aaatataaaa aaaatctaac ctcaagtcaa ggcttttcta
     3241 tggaataagg aatggacagc agggggctgt ttcatatact gatgacctct ttatagccaa
     3301 cctttgttca tggcagccag catatgggca tatgttgcca aactctaaac caaatactca
     3361 ttctgatgtt ttaaatgatt tgccctccca tatgtccttc cgagtgagag acacaaaaaa
     3421 ttccaacaca ctattgcaat gaaaataaat ttcctttatt agccagaagt cagatgctca
     3481 aggggcttca tgatgtcccc ataatttttg gcagagggaa aaagatctca gtggtatttg
     3541 tgagccaggg cattggccac accagccacc accttctgat aggcagcctg cacctgagga
     3601 gtgcggccgg ccgcgattca cagatcttct tcagaaataa gtttttgttc caccggtcca
     3661 tcgccatctt ccagcaggcg caccattgcc cctgtttcac tatccaggtt acggatatag
     3721 ttcatgacaa tatttacatt ggtccagcca ccagcttgca tgatctccgg tattgaaact
     3781 ccagcgcggg ccatatctcg cgcggctccg acacgggcac tgtgtccaga ccaggccagg
     3841 tatctctgac cagagtcatc cttagcgccg taaatcaatc gatgagttgc ttcaaaaatc
     3901 ccttccaggg cgcgagttga tagctggctg gtggcagatg gcgcggcaac accatttttt
     3961 ctgacccggc aaaacaggta gttattcgga tcatcagcta caccagagac ggaaatccat
     4021 cgctcgacca gtttagttac ccccaggcta agtgccttct ctacacctgc ggtgctaacc
     4081 agcgttttcg ttctgccaat atggattaac attctcccac cgtcagtacg tgagatatct
     4141 ttaaccctga tcctggcaat ttcggctata cgtaacaggg tgttataagc aatccccaga
     4201 aatgccagat tacgtatatc ctggcagcga tcgctatttt ccatgagtga acgaacctgg
     4261 tcgaaatcag tgcgttcgaa cgctagagcc tgttttgcac gttcaccggc atcaacgttt
     4321 tcttttcgga tccgccgcat aaccagtgaa acagcattgc tgtcacttgg tcgtggcagc
     4381 ccggaccgac gatgaagcat gtttagctgg cccaaatgtt gctggatagt ttttactgcc
     4441 agaccgcgcg cctgaagata tagaagataa tcgcgaacat cttcaggttc tgcgggaaac
     4501 catttccggt tattcaactt gcaccatgcc gcccacgacc ggcaaacgga cagaagcatt
     4561 ttccaggtat gctcagaaaa cgcctggcga tccctgaaca tgtccatcag gttcttgcga
     4621 acctcatcac tcgttgcatc gaccggtaat gcaggcaaat tttggtgtac ggtcagtaaa
     4681 ttggccatgg ttgtggccat attatcatcg tgtttttcaa aggaaaacca cgtccccgtg
     4741 gttcgggggg cctagacgtt tttttaacct cgactaaaca catgtaaagc atgtgcaccg
     4801 aggccccaga tcagatccca tacaatgggg taccttctgg gcatccttca gccccttgtt
     4861 gaatacgctt gaggagagcc atttgactct ttccacaact atccaactca caacgtggca
     4921 ctggggttgt gccgcctttg caggtgtatc ttatacacgt ggcttttggc cgcagaggca
     4981 cctgtcgcca ggtggggggt tccgctgcct gcaaagggtc gctacagacg ttgtttgtct
     5041 tcaagaagct tccagaggaa ctgcttcctt cacgacattc aacagacctt gcattccttt
     5101 ggcgagaggg gaaagacccc taggaatgct cgtcaagaag acagggccag gtttccgggc
     5161 cctcacattg ccaaaagacg gca
//
