LOCUS       sequence-178835-        5987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human gamma Synuclein fused to the C-terminal part of 
            Venus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Eckermann K, Kugler S, Bahr M
  TITLE     Dimerization propensities of Synucleins are not predictive for 
            Synuclein aggregation.
  JOURNAL   Biochim Biophys Acta. 2015 Aug;1852(8):1658-64. doi: 
            10.1016/j.bbadis.2015.05.002. Epub 2015 May 8.
  PUBMED    25960149
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbadis.2015.05.002"; journalName: "Biochim Biophys Acta";
            date: "2015-08"; volume: "1852"; issue: "8"; pages: "1658-64"
COMMENT     Sequence Label: gamma Synuclein-VC
FEATURES             Location/Qualifiers
     source          1..5987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(36..53)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    42..266
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      312..740
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(399..418)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     609..630
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(749..769)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        754..1083
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      934..1069
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     996..1015
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1150..1944
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1204..1223)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1814..1833
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2118..2239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2155..2174)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2209..2228
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2288..2304)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2288..2304)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2301..2323)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2312..2328
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2336..2342,2343..2360,2361..2366))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2381..2402
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2519..2536)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2690..3278)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2770..2789)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3449..4240,4241..4309))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4072..4091
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4310..4414)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4489..4508)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4680..5059
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5060..5263
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5214..5234
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5308..5327
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5308..5326
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(5737..5987,1)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DLEKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     5925..5946
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtaatctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
       61 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
      121 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
      181 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
      241 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
      301 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      361 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      421 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      481 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      541 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      601 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      661 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      721 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
      781 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
      841 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      901 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
      961 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     1021 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     1081 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     1141 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     1201 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     1261 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     1321 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     1381 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     1441 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     1501 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     1561 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     1621 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     1681 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     1741 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     1801 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     1861 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     1921 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     1981 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     2041 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     2101 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     2161 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2221 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     2281 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     2341 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     2401 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     2461 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     2521 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2581 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2641 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2701 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2761 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2821 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2881 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2941 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3001 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3061 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3121 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3181 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3241 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3301 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3361 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3421 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     3481 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     3541 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     3601 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3661 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3721 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3781 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3841 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3901 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3961 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4021 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     4081 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     4141 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     4201 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     4261 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     4321 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     4381 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     4441 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     4501 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     4561 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     4621 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
     4681 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     4741 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4801 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     4861 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     4921 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     4981 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     5041 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     5101 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     5161 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     5221 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
     5281 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
     5341 ctagcgttta aacttaagat ggatgtcttc aagaagggct tctccatcgc caaggagggc
     5401 gtggtgggtg cggtggaaaa gaccaagcag ggggtgacgg aagcagctga gaagaccaag
     5461 gagggggtca tgtatgtggg agccaagacc aaggagaatg ttgtacagag cgtgacctca
     5521 gtggccgaga agaccaagga gcaggccaac gccgtgagcg aggctgtggt gagcagcgtc
     5581 aacactgtgg ccaccaagac cgtggaggag gcggagaaca tcgcggtcac ctccggggtg
     5641 gtgcgcaagg aggacttgag gccatctgcc ccccaacagg agggtgaggc atccaaagag
     5701 aaagaggaag tggcagagga ggcccagagt gggggagacc tcgagaagaa cggcatcaag
     5761 gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac
     5821 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     5881 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     5941 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaa
//
