LOCUS       sequence-178853-        7210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suicide vector for integration of a constitutively expressed TetR by
            promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and
            4861702 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Lim B, Zimmermann M, Barry NA, Goodman AL
  TITLE     Engineered Regulatory Systems Modulate Gene Expression of Human 
            Commensals in the Gut.
  JOURNAL   Cell. 2017 Apr 20;169(3):547-558.e15. doi: 
            10.1016/j.cell.2017.03.045.
  PUBMED    28431252
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.03"; journalName: "Cell"; date: "2017-04-20- 
            20"; volume: "169"; issue: "3"; pages: "547-558.e15"
COMMENT     Sequence Label: pExchange_tdk_BT1311p-tetR-1
FEATURES             Location/Qualifiers
     source          1..7210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(30..821,822..890))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     653..672
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             4713..5336
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4786..4805)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     rep_origin      6441..6829
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            7000..7109
                     /note="incP origin of transfer"
ORIGIN
        1 acgcgttatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
       61 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
      601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
      661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
      721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
      781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      901 tttcaatcat ggccgcggga ttaaaagtcg gggattggtg aacaaaaagg tgtttctctc
      961 tttaagagaa atatcgtttt gctaaacagt tgatattgag gtatcatttt atcgtaaaag
     1021 acatttttgc tcaacaattg cttgacggaa atcaacaaat tttagcattt tgtaaaaaag
     1081 tcgctatata atttggtgaa ttggagttat tttcatattt ttgcatcccg aagagtttct
     1141 cttaaagaga gaaacatctt ttgcatacct tttccgaccg aatttttatg tcgtaaagag
     1201 gggctttgca gggggtggac tcagaaagat gagaatagat gactattgta gttgaaacac
     1261 atagaaagtt gctgatatac agaccgatac gcatatcggg atgaaccatg agtacgttct
     1321 tttctcaaaa aacataaata ttcgaaaaga gatgcaataa attaaggaga ggttataatg
     1381 aacaaagtaa atataaaaga tagtcaaaat tttattactt caaaatatca catagaaaaa
     1441 ataatgaatt gcataagttt agatgaaaaa gataacatct ttgaaatagg tgcagggaaa
     1501 ggtcatttta ctgctggatt ggtaaagaga tgtaattttg taacggcgat agaaattgat
     1561 tctaaattat gtgaggtaac tcgtaataag ctcttaaatt atcctaacta tcaaatagta
     1621 aatgatgata tactgaaatt tacatttcct agccacaatc catataaaat atttggcagc
     1681 ataccttaca acataagcac aaatataatt cgaaaaattg tttttgaaag ttcagccaca
     1741 ataagttatt taatagtgga atatggtttt gctaaaatgt tattagatac aaacagatca
     1801 ctagcattgc tgttaatggc agaggtagat atttctatat tagcaaaaat tcctaggtat
     1861 tatttccatc caaaacctaa agtggatagc acattaattg tattaaaaag aaagccagca
     1921 aaaatggcat ttaaagagag aaaaaaatat gaaacttttg taatgaaatg ggttaacaaa
     1981 gagtacgaaa aactgtttac aaaaaatcaa tttaataaag ctttaaaaca tgcgagaata
     2041 tatgatataa acaatattag tttcgaacaa tttgtatcgc tatttaatag ttataaaata
     2101 tttaacggct aaaaacaata ggccacatgc aactgtaaat gtttacgcgt cctcggtacc
     2161 gcttcttcca caacagtctg cggttcctgt actatcacag gttcatcttc tcccggtgct
     2221 gtcgcttcat tctcttccac gaccttctct tctatcaccg gacattcaac ttcctctatt
     2281 acaggggctt gttcttcaac aatgggggct tcactttccg cttccgctac aggagaaggc
     2341 gaggcttcca ccggcacagc acttatctct tccgacaact gttccaaacg ctcctgcata
     2401 cgtaggatgc tccgcttcaa cagttcagac aaagtctgag tcggctcttt agaaaacgta
     2461 ttcatgagta gcttcagctc atgaacatcc agctcaatat ctgttaatag cttctgtttc
     2521 atttccatcc attgttatta tggtgcgaat gtaggaataa tttatgcaat aacttaaaat
     2581 aatggataat ttaacagttc ttgtacatcg aattgtttac ctttgtccac tcatttattt
     2641 aatcaagaaa gcacaaaaat cacatggtat tattttcaga agaccacata caagagacca
     2701 gaagaagagg tagaatagag gtaatctgcg gttcaatgtt ctccggaaag acggaggaat
     2761 tgattcgtag gatgaagaga gccaagttcg cacgccagcg tgtagagata ttcaaaccgg
     2821 ccatcgacac ccgttattcg gaagaagacg tggtatcaca cgacagtcat tccatcgcgt
     2881 ccacccctat cgattcatcg gctagtatcc tgttgttcac ttctgaaata gatgtagtcg
     2941 gcatcgacga agcacagttc tttgatgacg gactgattga cgtatgcagg cagcttgcca
     3001 ataacggcat ccgtgtcatc atcgccggac tggatatgga ctttaaaggg aatcccttcg
     3061 gaccgatgcc tcaattatgt gccattgccg atgaagtttc caaagttcat gcgatctgcg
     3121 tgaagtgcgg tgatctggca tctttctctc atcgtaccgt caagaatgac aagcaggtac
     3181 ttctaggaga gacagccgaa tacgagccgc tttgcaggga gtgctacctc cgtgcacggg
     3241 gagaggacgg acagaagata taaactctat ttaaaacata tatggaaaga aagaagataa
     3301 cattcgagtc gacggtatcg ataagcttga tatcgaattc ctgcaatgcg aatgtagagg
     3361 ggaaaataga tgggaataat attagtttta ttattccaga tggattggag aacttgacgt
     3421 ttgcgcctac tattgttatt tctgataaag caacaataac tcctgcttct ggagtagaac
     3481 aagatttttc atctcctgta atatacacag tgacttccga agatggaatt gtaactacta
     3541 aatatacagt ttctgtatca ggtcaaataa gagattttgg attcgatgaa tgggagacta
     3601 tcaaatcatc tacatcgggt tctacagaac aatatgatgt tcctaaagga ctttatggta
     3661 ctagtaatcc gggaattatg acaattaacg agatgtttgg ttcaaatctt ccagagctgt
     3721 tctcatatac agtttctcct gtagaaggac aaagtggtaa agcagctaaa ttgcaaacag
     3781 tttatactta catgttttta aatggtatgg attttaatgc attattagga gggttggtac
     3841 cttatgtaac agcaggctca ttatttacag gtatctttaa aactgatatg aataatactt
     3901 tgaatagtac aaagtttggt gttccttttg ttggggaacc tgctactttt actggctatt
     3961 ataaatatac tccagggaca acatattata ataataaaaa tgaagtcgta gctgataaaa
     4021 cagatgaatg ttcaatatat gcagttttgt atgaagaact tttggatgaa gagaataaca
     4081 acattccgtt gaatggagac tacaatgatc ctgatctata tattggaagc tctaaacgta
     4141 ttgtaatgaa ggccgttttg gaagatggaa cagcgaagag tgattggact aagttctcta
     4201 tcccatttaa attattggaa gggaaaagtt atgatgcaac taaaaaatat tatttagcta
     4261 ttgtgtgttc ctcaagtgct gaaggagact actttatggg cgctcctggt agtaccttat
     4321 ttattgatag ttttacggta acacctcaat agtgtgttga gtataagaaa tatataacag
     4381 aaaaagccgt taattccaat tgtatacatt aatgatctgg aagaagcaat gaaagctgct
     4441 gttaagtctc cgaatcaggt attgttcctg acaggtgtat tcccatccgg taaacgcgga
     4501 tactttgcag ttgatctgac tcaggaataa attataaatt aaggtaagaa gattgtagga
     4561 taagctaatg aaatagaaaa aggatgccgt cacacaactt gtcggcattc ttttttgttt
     4621 tattagttga aaatatagtg aaaaagttgc ctaaatatgt atgttaacaa attatttgtc
     4681 gtaactttgc actccaaatc tgtttttaaa gaatgtctag attagataaa agtaaagtga
     4741 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac
     4801 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg
     4861 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt
     4921 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt
     4981 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt
     5041 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg
     5101 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag
     5161 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat
     5221 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc
     5281 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tcttaatgca
     5341 attaaaatta acggcttttt tagttgtttt tatttaaata aattgtagtg ttttctctag
     5401 aacgcatatc cgaatcctat attcaaataa ataggataca tagcgaatga gatggtatca
     5461 aaatcctttt tgaagatatc gttcaagccc catgttaaat cagcatacac atttaaatgt
     5521 ttgaatgctc tccactcacc gcctagttgc aatccccatt ggaatttacg cagattgtca
     5581 gagaaatcat aagtagcaat gctttcacca gtaaaatcta ctcgcgaacc tgtctgatcc
     5641 ggagtacgca agtgaccttc atatacatgt ccggagaagt ttctttcaat aagataagat
     5701 acatacggac ctgctgagac tttccaacgt ttgcttactt tatagttggc aactactggg
     5761 atagtcagat aagagttttt gactttagtc tttacaccac cggtccataa gccttgtaac
     5821 tctccaccgt tggtgttgat aatcttcatc ccatagttct ttactgtagc ttctgtggtc
     5881 atattcttgt tttcaagtcg tacccctaca gtcattcccc atttcgtgtc aaaccattta
     5941 gttgcgtttc cttcaattga aatggcgagg ccaccagggg catagctatc tattttgcga
     6001 atttcttcag gtagaggcaa tggagaagtt cctccaatac tgaaacctgc cttgattcca
     6061 tattcccatc cgtgtaagta cgaccagatg ataccttgat ttctttcttc ttgcgcctgc
     6121 gctgaaagtg tacttactaa tagtagtaca gctataatat tgagtaattt atatcttttc
     6181 attttcttgt ttttttagtc ttcgcaaata agttctactt catcgacata cagtgtactg
     6241 cctactgctc ccttaaaata agcaccgtct acactggacg aaagcactat acctaatttg
     6301 tattttccat tctttaattt ggtatcatca attactcgtc cttttaccgg ttcgaaagga
     6361 atttcaaaat cagtccattg atctgattca acaacatctt ccggtttaat tcgcgccacc
     6421 gcggtggagg ggaattccca tgtcagccgt taagtgttcc tgtgtcactc aaaattgctt
     6481 tgagaggctc taagggcttc tcagtgcgtt acatccctgg cttgttgtcc acaaccgtta
     6541 aaccttaaaa gctttaaaag ccttatatat tctttttttt cttataaaac ttaaaacctt
     6601 agaggctatt taagttgctg atttatatta attttattgt tcaaacatga gagcttagta
     6661 cgtgaaacat gagagcttag tacgttagcc atgagagctt agtacgttag ccatgagggt
     6721 ttagttcgtt aaacatgaga gcttagtacg ttaaacatga gagcttagta cgtgaaacat
     6781 gagagcttag tacgtactat caacaggttg aactgctgat cttcagatcc tctacgccgg
     6841 acgcatcgtg gccggatcaa ttccgttttc cgctgcataa ccctgcttcg gggtcattat
     6901 agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt
     6961 tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg
     7021 cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa
     7081 cgggaatcct gctctgcgag gctggccggc taccgccggc gtaacagatg agggcaagcg
     7141 gatggctgat gaaaccaagc caaccaggaa gggcagccca cctatcacgg aattgatccc
     7201 cctcgaattg
//
